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. 1983 Feb;78(2):441–445. doi: 10.1111/j.1476-5381.1983.tb09409.x

Effect of steroids on beta-adrenoceptor-mediated relaxation of pig bronchus.

P S Foster, R G Goldie, J W Paterson
PMCID: PMC2044699  PMID: 6299446

Abstract

1--Progesterone, testosterone (40 microM), cortisol and cortisol hemisuccinate (80 microM) caused 6-8 fold potentiations of (+/-)-isoprenaline (Iso)-induced relaxations of pig bronchus while several other steroids caused smaller potentiations or had no effect. 2--17 beta-Oestradiol (40 microM) increased the potency of Iso, (-)-adrenaline (Adr) and (-)-noradrenaline (NA) by 10.6, 2.3 and 2.6 fold respectively but had no significant effect on the potency of fenoterol (Fen). 3--Inhibition of catechol-O-methyl transferase (COMT) with U-0521 (30 microM) caused a 6 fold increase in the potency of Iso but failed to alter the potency of Adr, NA or Fen. The extraneuronal uptake inhibitor normetanephrine (50 microM) caused significant 2 fold increases in the potency of Iso and Adr but did not potentiate the responses to NA or Fen. 4--In preparations where the potency of Iso had already been increased by U-0521 (30 microM) or by normetanephrine, 17 beta-oestradiol produced no significant further increase in potency. These results indicate that steroid-induced increases in the potency of catecholamines in pig bronchus can be explained in terms of inhibition of COMT or extraneuronal uptake or both.

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Selected References

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  1. Besse J. C., Bass A. D. Potentiation by hydrocortisone of responses to catecholamines in vascular smooth muscle. J Pharmacol Exp Ther. 1966 Nov;154(2):224–238. [PubMed] [Google Scholar]
  2. Brine F., Cornish E. J., Miller R. C. Effects of uptake inhibitors on responses of sheep coronary arteries to catecholamines and sympathetic nerve stimulation. Br J Pharmacol. 1979 Dec;67(4):553–561. doi: 10.1111/j.1476-5381.1979.tb08701.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collins J. V., Clark T. J., Harris P. W., Townsend J. Intravenous corticosteroids in treatment of acute bronchial asthma. Lancet. 1970 Nov 21;2(7682):1047–1049. doi: 10.1016/s0140-6736(70)90283-7. [DOI] [PubMed] [Google Scholar]
  4. Cornish E. J., Goldie R. G. Metabolism of [3H]-(+/-)-isoprenaline by isolated atria and coronary arteries of the kitten. Br J Pharmacol. 1980 Mar;68(3):499–504. doi: 10.1111/j.1476-5381.1980.tb14564.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cornish E. J., Goldie R. G., Miller R. C. Catecholamine uptake by isolated coronary arteries and atria of the kitten. Br J Pharmacol. 1978 Jul;63(3):445–456. doi: 10.1111/j.1476-5381.1978.tb07796.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ellul-Micallef R., Borthwick R. C., McHardy G. J. The effect of oral prednisolone on gas exchange in chronic bronchial asthma. Br J Clin Pharmacol. 1980 May;9(5):479–482. doi: 10.1111/j.1365-2125.1980.tb05843.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ellul-Micallef R., Fenech F. F. Effect of intravenous prednisolone in asthmatics with diminished adrenergic responsiveness. Lancet. 1975 Dec 27;2(7948):1269–1271. doi: 10.1016/s0140-6736(75)90608-x. [DOI] [PubMed] [Google Scholar]
  8. FRITZ I., LEVINE R. Action of adrenal cortical steroids and nor-epinephrine on vascular responses of stress in adrenalectomized rats. Am J Physiol. 1951 May;165(2):456–465. doi: 10.1152/ajplegacy.1951.165.2.456. [DOI] [PubMed] [Google Scholar]
  9. Gillespie J. S. Uptake of noradrenaline by smooth muscle. Br Med Bull. 1973 May;29(2):136–141. doi: 10.1093/oxfordjournals.bmb.a070983. [DOI] [PubMed] [Google Scholar]
  10. Goldie R. G. Effect of cortisol-induced inhibition of extraneuronal uptake on responses to isoprenaline in the kitten. Clin Exp Pharmacol Physiol. 1980 May-Jun;7(3):325–328. doi: 10.1111/j.1440-1681.1980.tb00077.x. [DOI] [PubMed] [Google Scholar]
  11. Goldie R. G., Paterson J. W. Catecholamine transport in isolated lung parenchyma of pig. Br J Pharmacol. 1982 Aug;76(4):507–513. doi: 10.1111/j.1476-5381.1982.tb09247.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goldie R. G., Paterson J. W., Wale J. L. Pharmacological responses of human and porcine lung parenchyma, bronchus and pulmonary artery. Br J Pharmacol. 1982 Aug;76(4):515–521. doi: 10.1111/j.1476-5381.1982.tb09248.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goldie R. G. The effects of hydrocortisone on responses to and extraneuronal uptake of (-)-isoprenaline in cat and guinea-pig atria. Clin Exp Pharmacol Physiol. 1976 May-Jun;3(3):225–233. doi: 10.1111/j.1440-1681.1976.tb02668.x. [DOI] [PubMed] [Google Scholar]
  14. Guldberg H. C., Marsden C. A. Catechol-O-methyl transferase: pharmacological aspects and physiological role. Pharmacol Rev. 1975 Jun;27(2):135–206. [PubMed] [Google Scholar]
  15. Hackney J. F., Szentivanyi A. Response of isolated guinea-pig tracheal muscle to glucocorticoid and non-glucocorticoid succinates. Arch Int Pharmacodyn Ther. 1980 Mar;244(1):4–20. [PubMed] [Google Scholar]
  16. Iversen L. L., Salt P. J. Inhibition of catecholamine Uptake-2 by steroids in the isolated rat heart. Br J Pharmacol. 1970 Nov;40(3):528–530. doi: 10.1111/j.1476-5381.1970.tb10637.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kalsner S. Steroid potentiation of responses to sympathomimetic amines in aortic strips. Br J Pharmacol. 1969 Jul;36(3):582–593. doi: 10.1111/j.1476-5381.1969.tb08013.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kaumann A. J. Potentiation of the effects of isoprenaline and noradrenaline by hydrocortisone in cat heart muscle. Naunyn Schmiedebergs Arch Pharmacol. 1972;273(1):134–153. doi: 10.1007/BF00508086. [DOI] [PubMed] [Google Scholar]
  19. Logsdon P. J., Middleton E., Jr, Coffey R. G. Stimulation of leukocyte adenyl cyclase by hydrocortisone and isoproterenol in asthmatic and nonasthmatic subjects. J Allergy Clin Immunol. 1972 Jul;50(1):45–56. doi: 10.1016/0091-6749(72)90078-4. [DOI] [PubMed] [Google Scholar]
  20. Major H., Sauerwein I., Graefe K. H. Kinetics of the uptake and metabolism of 3H-(+/-) isoprenaline in the rat submaxillary gland. Naunyn Schmiedebergs Arch Pharmacol. 1978 Oct;305(1):51–63. doi: 10.1007/BF00497006. [DOI] [PubMed] [Google Scholar]
  21. Marone G., Lichtenstein L. M., Plaut M. Hydrocortisone and human lymphocytes: increases in cyclic adenosine 3':5'-monophosphate and potentiation of adenylate cyclase-activating agents. J Pharmacol Exp Ther. 1980 Nov;215(2):469–478. [PubMed] [Google Scholar]
  22. Mireylees S. E., Foster R. W. Letter: 3-methoxyisoprenaline: a potent selective Uptake2 inhibitor. J Pharm Pharmacol. 1973 Oct;25(10):833–835. doi: 10.1111/j.2042-7158.1973.tb09954.x. [DOI] [PubMed] [Google Scholar]
  23. Pun L. Q., McCulloch M. W., Rand M. J. The effect of hydrocortisone on the bronchodilator activity of sympathomimetic amines and on the uptake of isoprenaline in the isolated guinea-pig trachea. Eur J Pharmacol. 1973 May;22(2):162–168. doi: 10.1016/0014-2999(73)90007-1. [DOI] [PubMed] [Google Scholar]
  24. Rimon G., Hanski E., Braun S., Levitzki A. Mode of coupling between hormone receptors and adenylate cyclase elucidated by modulation of membrane fluidity. Nature. 1978 Nov 23;276(5686):394–396. doi: 10.1038/276394a0. [DOI] [PubMed] [Google Scholar]
  25. Salt P. J. Inhibition of noradrenaline uptake 2 in the isolated rat heart by steroids, clonidine and methoxylated phenylethylamines. Eur J Pharmacol. 1972 Dec;20(3):329–340. doi: 10.1016/0014-2999(72)90194-x. [DOI] [PubMed] [Google Scholar]
  26. Schmid P. G., Eckstein J. W., Abboud F. M. Comparison of effects of deoxycorticosterone and dexamethasone on cardiovascular responses to norepinephrine. J Clin Invest. 1967 Apr;46(4):590–598. doi: 10.1172/JCI105560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shenfield G. M., Hodson M. E., Clarke S. W., Paterson J. W. Interaction of corticosteroids and catecholamines in the treatment of asthma. Thorax. 1975 Aug;30(4):430–435. doi: 10.1136/thx.30.4.430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Trendelenburg U. A kinetic analysis of the extraneuronal uptake and metabolism of catecholamines. Rev Physiol Biochem Pharmacol. 1980;87:33–115. doi: 10.1007/BFb0030896. [DOI] [PubMed] [Google Scholar]
  29. Yard A. C., Kadowitz P. J. Studies on the mechanism of hydrocortisone potentiation of vasoconstrictor responses to epinephrine in the anesthetized animal. Eur J Pharmacol. 1972 Oct;20(1):1–9. doi: 10.1016/0014-2999(72)90209-9. [DOI] [PubMed] [Google Scholar]

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