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. 1983 Jun;79(2):543–552. doi: 10.1111/j.1476-5381.1983.tb11029.x

Rapid degradation of [3H]-substance p in guinea-pig ileum and rat vas deferens in vitro.

S P Watson
PMCID: PMC2044881  PMID: 6197123

Abstract

The degradation of [3H]-substance P was monitored in guinea-pig ileum and rat vas deferens. Substantial and rapid metabolism occurred in both tissues within the time course of the pharmacological responses; for example, after 1 min more than 50% of the extracted tritium from both tissues was present in the form of metabolites. [3H]-inulin was used to estimate the rate of equilibration of the extracellular space of guinea-pig ileum longitudinal muscle and rat vas deferens with the bath fluid; half-lives of 30 s and 5 min respectively were observed. These two factors combine to give different concentrations of substance P in the biophase of the tissues. The peak concentration of substance P reached in the guinea-pig ileum longitudinal muscle was approximately five times higher than in the rat vas deferens. An analogue of substance P, [3H]-DiMe-C7, was found to be stable in both tissues. These results are discussed in the light of the suggested existence of multiple receptors for substance P, based on agonist potency differences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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