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. 1983 Nov;80(3):477–484. doi: 10.1111/j.1476-5381.1983.tb10718.x

The contractile response to and the release of noradrenaline by transmural nerve stimulation in the guinea-pig vas deferens and a comparison with the response to noradrenaline.

J S Gillespie, I M Macrae
PMCID: PMC2045005  PMID: 6416341

Abstract

The release of noradrenaline from the guinea-pig vas deferens by transmural stimulation was measured electrochemically after separation by h.p.l.c. Variations in release with different durations of stimulation were then compared with the mechanical response during stimulation. During long periods of 270 s of stimulation at 10 Hz the average release of noradrenaline in the final 250 s remained at about 50% of that released during the first 20 s yet the mechanical response throughout the later time declined to near zero. Superfusion with constant concentrations of noradrenaline produced mechanical responses similar to those from transmural nerve stimulation, an initial rapid contraction declining to a low level of maintained activity. During the later period, when the response to infused noradrenaline was low, the twitch response to transmural stimulation was potentiated. Phentolamine 10(-6)M reduced the potentiation of the twitch response to transmural stimulation by noradrenaline and propranolol 10(-6)M enhanced the potentiation. These results suggest that the decline in the adrenergic component of the response to prolonged transmural nerve stimulation is not the result of a decline in noradrenaline release but mainly due to desensitization to the contractile effect of noradrenaline. Since the similar desensitization produced by infused noradrenaline does not reduce the twitch response to transmural nerve stimulation it is unlikely that the transmitter responsible for that component is noradrenaline.

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Selected References

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  1. ANTON A. H., SAYRE D. F. A study of the factors affecting the aluminum oxide-trihydroxyindole procedure for the analysis of catecholamines. J Pharmacol Exp Ther. 1962 Dec;138:360–375. [PubMed] [Google Scholar]
  2. Ambache N., Dunk L. P., Verney J., Zar M. A. Inhibition of post-ganglionic motor transmission in vas deferens by indirectly acting sympathomimetic drugs. J Physiol. 1972 Dec;227(2):433–456. doi: 10.1113/jphysiol.1972.sp010041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ambache N., Zar M. A. Evidence against adrenergic motor transmission in the guinea-pig vas deferens. J Physiol. 1971 Jul;216(2):359–389. doi: 10.1113/jphysiol.1971.sp009530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown C. M., McGrath J. C., Summers R. J. The effects of alpha-adrenoceptor agonists and antagonists on responses of transmurally stimulated prostatic and epididymal portions of the isolated vas deferens of the rat. Br J Pharmacol. 1979 Aug;66(4):553–564. doi: 10.1111/j.1476-5381.1979.tb13694.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doggrell S. A. Prazosin selectively inhibits the responses to field stimulation in the rat vas deferens. Eur J Pharmacol. 1981 May 22;71(4):447–453. doi: 10.1016/0014-2999(81)90189-8. [DOI] [PubMed] [Google Scholar]
  6. Duncan M. E., McGrath J. C. Proceedings: Observations on the origin of the complex mechanical response to motor nerve stimulation of the rat vas deferens. J Physiol. 1976 Jul;259(1):54P–55P. [PubMed] [Google Scholar]
  7. Hedqvist P., Von Euler U. S. Inhibition by alpha- and beta-adrenoceptors of the twitch response to transmural stimulation in the guinea-pig vas deferens. Eur J Pharmacol. 1976 Nov;40(1):153–162. doi: 10.1016/0014-2999(76)90365-4. [DOI] [PubMed] [Google Scholar]
  8. Hirst G. D., Neild T. O. Evidence for two populations of excitatory receptors for noradrenaline on arteriolar smooth muscle. Nature. 1980 Feb 21;283(5749):767–768. doi: 10.1038/283767a0. [DOI] [PubMed] [Google Scholar]
  9. Hotta Y. Some properties of the junctional and extrajunctional receptors in the vas deferens of the guinea-pig. Agents Actions. 1969 Nov;1(2):13–21. doi: 10.1007/BF01977661. [DOI] [PubMed] [Google Scholar]
  10. Keller R., Oke A., Mefford I., Adams R. N. Liquid chromatographic analysis of catecholamines routine assay for regional brain mapping. Life Sci. 1976 Oct 1;19(7):995–1003. doi: 10.1016/0024-3205(76)90290-3. [DOI] [PubMed] [Google Scholar]
  11. McGrath J. C. Adrenergic and 'non-adrenergic' components in the contractile response of the vas deferens to a single indirect stimulus. J Physiol. 1978 Oct;283:23–39. doi: 10.1113/jphysiol.1978.sp012486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sjöstrand N. O. Effects of adrenaline, noradrenaline and isoprenaline on the electrical and mechanical responses of the guinea-pig vas deferens to nerve stimulation. Acta Physiol Scand. 1973 Sep;89(1):10–18. doi: 10.1111/j.1748-1716.1973.tb05492.x. [DOI] [PubMed] [Google Scholar]
  13. Swedin G. Studies on neurotransmission mechanisms in the rat and guinea-pig vas deferens. Acta Physiol Scand Suppl. 1971;369:1–34. [PubMed] [Google Scholar]
  14. Wadsworth R. M. Excitatory and inhibitory effects of noradrenaline on the isolated guinea-pig vas deferens. Clin Exp Pharmacol Physiol. 1974 Mar-Apr;1(2):135–145. doi: 10.1111/j.1440-1681.1974.tb00534.x. [DOI] [PubMed] [Google Scholar]
  15. von Euler U. S., Hedqvist P. Evidence for an alpha- and beta2-receptor mediated inhibition of the twitch response in the guinea pig vas deferens by noradrenaline. Acta Physiol Scand. 1975 Apr;93(4):572–573. doi: 10.1111/j.1748-1716.1975.tb05853.x. [DOI] [PubMed] [Google Scholar]

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