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. 1983 Dec;80(4):671–679. doi: 10.1111/j.1476-5381.1983.tb10057.x

Effects of adrenoceptor agonists and antagonists on smooth muscle cells and neuromuscular transmission in the guinea-pig renal artery and vein.

Y Makita
PMCID: PMC2045049  PMID: 6100844

Abstract

In the guinea-pig renal artery and vein, the membrane potential was -66.8 mV and -46.8 mV, the length constant 0.54 mm and 0.43 mm, and the time constant 240 ms and 98 ms, respectively. The maximum slope of the depolarization produced by a 10 fold increase [K]o was 46 mV in the renal artery and 39 mV in the renal vein. Noradrenaline (NA over 5 X 10(-7)M in the artery and over 10(-7)M in the vein) depolarized the membrane and slightly reduced the membrane resistance, assessed from relative changes in the amplitude of electrotonic potential. The action of NA was suppressed by prazosin in the artery but by yohimbine in the vein, i.e. the alpha 1-adrenoceptor is present in the extrajunctional muscle membrane in the renal artery while the alpha 2-adrenoceptor is present in the renal vein. Dopamine and isoprenaline did not modify the membrane properties. In the renal artery, repetitive perivascular nerve stimulation (0.1 ms, 50 Hz, 5 shocks) evoked excitatory junction potential (e.j.p.). Applications of guanethidine (10(-6) M) or tetrodotoxin (3 X 10(-7) M) abolished the generation of the e.j.p.. Low concentrations of phentolamine (5 X 10(-7) M), prazosin (10(-7) M) and yohimbine (5 X 10(-7) M) enhanced the e.j.p. amplitude, while high concentrations of phentolamine (10(-5) M) and prazosin (greater than 10(-5) M) reduced the amplitude of e.j.p.s. NA, dopamine and clonidine consistently suppressed the amplitude of e.j.ps, at any given concentration over 10(-7) M. Spontaneous generated miniature e.j.ps (m.e.j.ps) were recorded on rare occasions. Phentolamine and yohimbine both at 5 x 10(-7) M and prazosin 10(-7) M increased the appearance of m.e.j.ps.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Abe Y., Tomita T. Cable properties of smooth muscle. J Physiol. 1968 May;196(1):87–100. doi: 10.1113/jphysiol.1968.sp008496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asada H., Nanjo T., Itoh T., Suzuki H., Kuriyama H. Effects of 3,4-dihydro-8-(2-hydroxy-3-isopropylaminopropoxy)-3-nitroxy-2H-1-bezopyran (K-351) on smooth muscle cells and neuromuscular transmission in guinea-pig vascular tissues. J Pharmacol Exp Ther. 1982 Nov;223(2):560–572. [PubMed] [Google Scholar]
  3. Barajas L. Innervation of the renal cortex. Fed Proc. 1978 Apr;37(5):1192–1201. [PubMed] [Google Scholar]
  4. Burger B. M., Hopkins T., Tulloch A., Hollenberg N. K. The role of angiotensin in the canine renal vascular response to barbiturate anesthesia. Circ Res. 1976 Mar;38(3):196–202. doi: 10.1161/01.res.38.3.196. [DOI] [PubMed] [Google Scholar]
  5. Chapman B. J., Horn N. M., Robertson M. J. Renal blood-flow changes during renal nerve stimulation in rats treated with alpha-adrenergic and dopaminergic blockers. J Physiol. 1982 Apr;325:67–77. doi: 10.1113/jphysiol.1982.sp014136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coote J. H., Johns E. J., Macleod V. H., Singer B. Effect of renal nerve stimulation, renal blood flow and adrenergic blockade on plasma renin activity in the cat. J Physiol. 1972 Oct;226(1):15–36. doi: 10.1113/jphysiol.1972.sp009971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DiSalvo J., Fell C. Changes in renal blood flow during renal nerve stimulation. Proc Soc Exp Biol Med. 1971 Jan;136(1):150–153. doi: 10.3181/00379727-136-35215. [DOI] [PubMed] [Google Scholar]
  8. Fujiwara S., Itoh T., Suzuki H. Membrane properties and excitatory neuromuscular transmission in the smooth muscle of dog cerebral arteries. Br J Pharmacol. 1982 Oct;77(2):197–208. doi: 10.1111/j.1476-5381.1982.tb09286.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goldberg L. I., Volkman P. H., Kohli J. D. A comparison of the vascular dopamine receptor with other dopamine receptors. Annu Rev Pharmacol Toxicol. 1978;18:57–79. doi: 10.1146/annurev.pa.18.040178.000421. [DOI] [PubMed] [Google Scholar]
  10. Gomer S. K., Zimmerman B. G. Determination of sympathetic vasodilator responses during renal nerve stimulation. J Pharmacol Exp Ther. 1972 Apr;181(1):75–82. [PubMed] [Google Scholar]
  11. Halushka P. V., Hoffmann P. C. Distribution and metabolism of dopamine in guinea-pigs. J Pharm Pharmacol. 1968 Dec;20(12):943–946. doi: 10.1111/j.2042-7158.1968.tb09678.x. [DOI] [PubMed] [Google Scholar]
  12. Hirst G. D., Neild T. O. Localization of specialized noradrenaline receptors at neuromuscular junctions on arterioles of the guinea-pig. J Physiol. 1981;313:343–350. doi: 10.1113/jphysiol.1981.sp013669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hirst G. D., Neild T. O., Silverberg G. D. Noradrenaline receptors on the rat basilar artery. J Physiol. 1982 Jul;328:351–360. doi: 10.1113/jphysiol.1982.sp014268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hollenberg N. K., Adams D. F., Rashid A., Epstein M., Abrams H. L., Merrill J. P. Renal vascular response to salt restriction in normal man. Evidence against adrenergic mediation. Circulation. 1971 Jun;43(6):845–851. doi: 10.1161/01.cir.43.6.845. [DOI] [PubMed] [Google Scholar]
  15. Holman M. E., Surprenant A. An electrophysiological analysis of the effects of noradrenaline and alpha-receptor antagonists on neuromuscular transmission in mammalian muscular arteries. Br J Pharmacol. 1980;71(2):651–661. doi: 10.1111/j.1476-5381.1980.tb10986.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ito Y., Kitamura K., Kuriyama H. Effects of acetylcholine and catecholamines on the smooth muscle cell of the porcine coronary artery. J Physiol. 1979 Sep;294:595–611. doi: 10.1113/jphysiol.1979.sp012948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Itoh T., Kuriyama H., Suzuki H. Excitation--contraction coupling in smooth muscle cells of the guinea-pig mesenteric artery. J Physiol. 1981 Dec;321:513–535. doi: 10.1113/jphysiol.1981.sp014000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Karashima T., Kuriyama H. Electrical properties of smooth muscle cell membrane and neuromuscular transmission in the guinea-pig basilar artery. Br J Pharmacol. 1981 Oct;74(2):495–504. doi: 10.1111/j.1476-5381.1981.tb09996.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kou K., Kuriyama H., Suzuki H. Effects of 3,4-dihydro-8-(2-hydroxy-3-isopropylaminopropoxy)-3-nitroxy-2H-1-benzopyran (K-351) on smooth muscle cells and neuromuscular transmission in the canine mesenteric artery. Br J Pharmacol. 1982 Dec;77(4):679–689. doi: 10.1111/j.1476-5381.1982.tb09346.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kuriyama H., Makita Y. Modulation of noradrenergic transmission in the guinea-pig mesenteric artery: an electrophysiological study. J Physiol. 1983 Feb;335:609–627. doi: 10.1113/jphysiol.1983.sp014554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kuriyama H., Oshima K., Sakamoto Y. The membrane properties of the smooth muscle of the guinea-pig portal vein in isotonic and hypertonic solutions. J Physiol. 1971 Aug;217(1):179–199. doi: 10.1113/jphysiol.1971.sp009565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kuriyama H., Suzuki H. Adrenergic transmissions in the guinea-pig mesenteric artery and their cholinergic modulations. J Physiol. 1981 Aug;317:383–396. doi: 10.1113/jphysiol.1981.sp013831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. McKenna O. C., Angelakos E. T. Adrenergic innervation of the canine kidney. Circ Res. 1968 Mar;22(3):345–354. doi: 10.1161/01.res.22.3.345. [DOI] [PubMed] [Google Scholar]
  24. NILSSON O. THE ADRENERGIC INNERVATION OF THE KIDNEY. Lab Invest. 1965 Jul;14:1392–1395. [PubMed] [Google Scholar]
  25. PAPPENHEIMER J. R. Central control of renal circulation. Physiol Rev Suppl. 1960 Apr;4:35–37. [PubMed] [Google Scholar]
  26. Surprenant A. A comparative study of neuromuscular transmission in several mammalian muscular arteries. Pflugers Arch. 1980 Jul;386(1):85–91. doi: 10.1007/BF00584192. [DOI] [PubMed] [Google Scholar]
  27. Suzuki H., Kuriyama H. Observation of quantal release of noradrenaline from vascular smooth muscles in potassium-free solution. Jpn J Physiol. 1980;30(4):665–670. doi: 10.2170/jjphysiol.30.665. [DOI] [PubMed] [Google Scholar]
  28. Taira N., Yabuuchi Y., Yamashita S. Profile of beta-adrenoceptors in femoral, superior mesenteric and renal vascular beds of dogs. Br J Pharmacol. 1977 Apr;59(4):577–583. doi: 10.1111/j.1476-5381.1977.tb07724.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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