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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Gene. 2007 Jun 7;400(1-2):44–51. doi: 10.1016/j.gene.2007.05.017

Fig. 5.

Fig. 5

Mutant LHX3 proteins contribute poorly to combinatorial activation of a nervous system transcription unit. A. A luciferase reporter gene containing a minimal PRL promoter under the control of the enhancer of the Hb9 transcription factor gene (Thaler et al., 2002) was transiently co-transfected into mouse P19 embryonal teratocarcinoma cells with the indicated combinations of wild type human LHX3 isoforms, NeuroM (NeuM), E47, and ISL1 transcription factor expression vectors. Promoter activity was assayed by measurement of luciferase activity after 48 hours. Activities are mean [light units/10 seconds/μg total protein] of triplicate assays ± S.E.M. The dotted line indicates activity with all factors except LHX3. B. Similar experiment testing mutant LHX3 proteins.