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. 1993 Apr;175(8):2475–2478. doi: 10.1128/jb.175.8.2475-2478.1993

Cloning and sequence determination of the valS gene, encoding valyl-tRNA synthetase in Lactobacillus casei.

B V Taylor 1, J Toy 1, T L Sit 1, A L Bognar 1
PMCID: PMC204542  PMID: 8468307

Abstract

The DNA sequence of the valS gene from Lactobacillus casei and the predicted amino acid sequence of its valyl-tRNA synthetase product have been determined. An open reading frame coding for a protein of 901 amino acids was found. A clone containing the intact L. casei valS gene functionally complemented the temperature-sensitive growth of the valS mutant strain 236c of Escherichia coli. The valS gene and the downstream folylpolyglutamate synthetase gene are transcribed in the same direction but are separated by a putative transcription terminator.

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Selected References

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