Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1993 May;175(9):2758–2761. doi: 10.1128/jb.175.9.2758-2761.1993

Identification of a phosphoenolpyruvate:fructose phosphotransferase system (fructose-1-phosphate forming) in Listeria monocytogenes.

W J Mitchell 1, J Reizer 1, C Herring 1, C Hoischen 1, M H Saier Jr 1
PMCID: PMC204581  PMID: 8478337

Abstract

Listeria monocytogenes is a gram-positive bacterium whose carbohydrate metabolic pathways are poorly understood. We provide evidence for an inducible phosphoenolpyruvate (PEP):fructose phosphotransferase system (PTS) in this pathogen. The system consists of enzyme I, HPr, and a fructose-specific enzyme II complex which generates fructose-1-phosphate as the cytoplasmic product of the PTS-catalyzed vectorial phosphorylation reaction. Fructose-1-phosphate kinase then converts the product of the PTS reaction to fructose-1,6-bisphosphate. HPr was shown to be phosphorylated by [32P]PEP and enzyme I as well as by [32P]ATP and a fructose-1,6-bisphosphate-activated HPr kinase like those found in other gram-positive bacteria. Enzyme I, HPr, and the enzyme II complex of the Listeria PTS exhibit enzymatic cross-reactivity with PTS enzyme constituents from Bacillus subtilis and Staphylococcus aureus.

Full text

PDF
2758

Images in this article

2760Image
on p.2760

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumann P., Baumann L. Catabolism of D-fructose and D-ribose by Pseudomonas doudoroffii. I. Physiological studies and mutant analysis. Arch Microbiol. 1975 Nov 7;105(3):225–240. doi: 10.1007/BF00447141. [DOI] [PubMed] [Google Scholar]
  2. Conner D. E., Brackett R. E., Beuchat L. R. Effect of temperature, sodium chloride, and pH on growth of Listeria monocytogenes in cabbage juice. Appl Environ Microbiol. 1986 Jul;52(1):59–63. doi: 10.1128/aem.52.1.59-63.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Farber J. M., Peterkin P. I. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev. 1991 Sep;55(3):476–511. doi: 10.1128/mr.55.3.476-511.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gauthier L., Mayrand D., Vadeboncoeur C. Isolation of a novel protein involved in the transport of fructose by an inducible phosphoenolpyruvate fructose phosphotransferase system in Streptococcus mutans. J Bacteriol. 1984 Nov;160(2):755–763. doi: 10.1128/jb.160.2.755-763.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gay P., Delobbe A. Fructose transport in Bacillus subtilis. Eur J Biochem. 1977 Oct 3;79(2):363–373. doi: 10.1111/j.1432-1033.1977.tb11817.x. [DOI] [PubMed] [Google Scholar]
  6. Mattoo R. L., Waygood E. B. An enzymatic method for [32P]phosphoenolpyruvate synthesis. Anal Biochem. 1983 Jan;128(1):245–249. doi: 10.1016/0003-2697(83)90372-x. [DOI] [PubMed] [Google Scholar]
  7. Parr T. R., Jr, Saier M. H., Jr The bacterial phosphotransferase system as a potential vehicle for the entry of novel antibiotics. Res Microbiol. 1992 Jun;143(5):443–447. doi: 10.1016/0923-2508(92)90089-7. [DOI] [PubMed] [Google Scholar]
  8. Pine L., Malcolm G. B., Brooks J. B., Daneshvar M. I. Physiological studies on the growth and utilization of sugars by Listeria species. Can J Microbiol. 1989 Feb;35(2):245–254. doi: 10.1139/m89-037. [DOI] [PubMed] [Google Scholar]
  9. Portnoy D. A., Chakraborty T., Goebel W., Cossart P. Molecular determinants of Listeria monocytogenes pathogenesis. Infect Immun. 1992 Apr;60(4):1263–1267. doi: 10.1128/iai.60.4.1263-1267.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reizer J., Novotny M. J., Hengstenberg W., Saier M. H., Jr Properties of ATP-dependent protein kinase from Streptococcus pyogenes that phosphorylates a seryl residue in HPr, a phosphocarrier protein of the phosphotransferase system. J Bacteriol. 1984 Oct;160(1):333–340. doi: 10.1128/jb.160.1.333-340.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reizer J., Peterkofsky A., Romano A. H. Evidence for the presence of heat-stable protein (HPr) and ATP-dependent HPr kinase in heterofermentative lactobacilli lacking phosphoenolpyruvate:glycose phosphotransferase activity. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2041–2045. doi: 10.1073/pnas.85.7.2041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reizer J. Regulation of sugar uptake and efflux in gram-positive bacteria. FEMS Microbiol Rev. 1989 Jun;5(1-2):149–156. doi: 10.1016/0168-6445(89)90019-3. [DOI] [PubMed] [Google Scholar]
  13. Reizer J., Saier M. H., Jr, Deutscher J., Grenier F., Thompson J., Hengstenberg W. The phosphoenolpyruvate:sugar phosphotransferase system in gram-positive bacteria: properties, mechanism, and regulation. Crit Rev Microbiol. 1988;15(4):297–338. doi: 10.3109/10408418809104461. [DOI] [PubMed] [Google Scholar]
  14. Reizer J., Sutrina S. L., Wu L. F., Deutscher J., Reddy P., Saier M. H., Jr Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli. J Biol Chem. 1992 May 5;267(13):9158–9169. [PubMed] [Google Scholar]
  15. Saier M. H., Jr, Feucht B. U., Mora W. K. Sugar phosphate: sugar transphosphorylation and exchange group translocation catalyzed by the enzyme 11 complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. J Biol Chem. 1977 Dec 25;252(24):8899–8907. [PubMed] [Google Scholar]
  16. Saier M. H., Jr, Reizer J. Proposed uniform nomenclature for the proteins and protein domains of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. J Bacteriol. 1992 Mar;174(5):1433–1438. doi: 10.1128/jb.174.5.1433-1438.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES