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. 1993 May;175(10):2895–2906. doi: 10.1128/jb.175.10.2895-2906.1993

Interaction between the antisense and target RNAs involved in the regulation of IncB plasmid replication.

K R Siemering 1, J Praszkier 1, A J Pittard 1
PMCID: PMC204607  PMID: 7684039

Abstract

Physical analysis of RNA I, the small antisense RNA which regulates the replication of IncB miniplasmid pMU720, showed that it is a highly structured molecule containing an imperfectly paired stem closed by a 6-base hairpin loop. Mutational studies revealed that a 3-base sequence in the hairpin loop is critical to the interaction between RNA I and its complementary target in the RepA mRNA (RNA II). Furthermore, a 2-base interior loop in the upper stem was found to play an important role in facilitating effective binding between RNA I and RNA II. From these analyses, a model describing the molecular mechanism of binding between RNA I and RNA II is proposed.

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Selected References

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