Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1993 May;175(10):3089–3095. doi: 10.1128/jb.175.10.3089-3095.1993

Binding of an Escherichia coli double-stranded DNA virus PRD1 to a receptor coded by an IncP-type plasmid.

M M Kotilainen 1, A M Grahn 1, J K Bamford 1, D H Bamford 1
PMCID: PMC204630  PMID: 8387995

Abstract

IncP plasmid RP1 Tra regions are needed to assemble the receptor for lipid-containing double-stranded DNA bacteriophage PRD1 on the cell surface. Using radioactively labeled phage and electron microscopic techniques, we showed that the surfaces of Salmonella typhimurium(RP1) and Escherichia coli(RP1) cells contained approximately 50 and 20 PRD1 binding sites, respectively. Expression of the receptor was growth phase dependent and was highest at late logarithmic or early stationary phase. The PRD1-resistant RP1 transposon mutants isolated were all Tra-, and the transposons were located in both the Tra1 and Tra2 regions.

Full text

PDF
3089

Images in this article

3092Image
on p.3092

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bamford D. H., Mindich L. Characterization of the DNA-protein complex at the termini of the bacteriophage PRD1 genome. J Virol. 1984 May;50(2):309–315. doi: 10.1128/jvi.50.2.309-315.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bamford D. H., Rouhiainen L., Takkinen K., Söderlund H. Comparison of the lipid-containing bacteriophages PRD1, PR3, PR4, PR5 and L17. J Gen Virol. 1981 Dec;57(Pt 2):365–373. doi: 10.1099/0022-1317-57-2-365. [DOI] [PubMed] [Google Scholar]
  3. Bamford D., Mindich L. Structure of the lipid-containing bacteriophage PRD1: disruption of wild-type and nonsense mutant phage particles with guanidine hydrochloride. J Virol. 1982 Dec;44(3):1031–1038. doi: 10.1128/jvi.44.3.1031-1038.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bamford J. K., Bamford D. H. Capsomer proteins of bacteriophage PRD1, a bacterial virus with a membrane. Virology. 1990 Aug;177(2):445–451. doi: 10.1016/0042-6822(90)90508-o. [DOI] [PubMed] [Google Scholar]
  5. Bamford J. K., Hänninen A. L., Pakula T. M., Ojala P. M., Kalkkinen N., Frilander M., Bamford D. H. Genome organization of membrane-containing bacteriophage PRD1. Virology. 1991 Aug;183(2):658–676. doi: 10.1016/0042-6822(91)90995-n. [DOI] [PubMed] [Google Scholar]
  6. Barth P. T., Grinter N. J., Bradley D. E. Conjugal transfer system of plasmid RP4: analysis by transposon 7 insertion. J Bacteriol. 1978 Jan;133(1):43–52. doi: 10.1128/jb.133.1.43-52.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bradley D. E. Morphological and serological relationships of conjugative pili. Plasmid. 1980 Sep;4(2):155–169. doi: 10.1016/0147-619x(80)90005-0. [DOI] [PubMed] [Google Scholar]
  8. Caldentey J., Bamford J. K., Bamford D. H. Structure and assembly of bacteriophage PRD1, and Escherichia coli virus with a membrane. J Struct Biol. 1990 Jul-Sep;104(1-3):44–51. doi: 10.1016/1047-8477(90)90056-i. [DOI] [PubMed] [Google Scholar]
  9. Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976 Jul 5;104(3):541–555. doi: 10.1016/0022-2836(76)90119-4. [DOI] [PubMed] [Google Scholar]
  10. Davis T. N., Muller E. D., Cronan J. E., Jr The virion of the lipid-containing bacteriophage PR4. Virology. 1982 Jul 30;120(2):287–306. doi: 10.1016/0042-6822(82)90031-9. [DOI] [PubMed] [Google Scholar]
  11. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. doi: 10.1128/jb.110.2.529-537.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Guiney D. G., Hasegawa P., Davis C. E. Plasmid transfer from Escherichia coli to Bacteroides fragilis: differential expression of antibiotic resistance phenotypes. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7203–7206. doi: 10.1073/pnas.81.22.7203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Heinemann J. A., Sprague G. F., Jr Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast. Nature. 1989 Jul 20;340(6230):205–209. doi: 10.1038/340205a0. [DOI] [PubMed] [Google Scholar]
  14. Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lessl M., Balzer D., Lurz R., Waters V. L., Guiney D. G., Lanka E. Dissection of IncP conjugative plasmid transfer: definition of the transfer region Tra2 by mobilization of the Tra1 region in trans. J Bacteriol. 1992 Apr;174(8):2493–2500. doi: 10.1128/jb.174.8.2493-2500.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lessl M., Balzer D., Pansegrau W., Lanka E. Sequence similarities between the RP4 Tra2 and the Ti VirB region strongly support the conjugation model for T-DNA transfer. J Biol Chem. 1992 Oct 5;267(28):20471–20480. [PubMed] [Google Scholar]
  17. Lundström K. H., Bamford D. H., Palva E. T., Lounatmaa K. Lipid-containing bacteriophage PR4: structure and life cycle. J Gen Virol. 1979 Jun;43(3):583–592. doi: 10.1099/0022-1317-43-3-583. [DOI] [PubMed] [Google Scholar]
  18. Lyra C., Savilahti H., Bamford D. H. High-frequency transfer of linear DNA containing 5'-covalently linked terminal proteins: electroporation of bacteriophage PRD1 genome into Escherichia coli. Mol Gen Genet. 1991 Aug;228(1-2):65–69. doi: 10.1007/BF00282449. [DOI] [PubMed] [Google Scholar]
  19. Matsunaga T., Nakamura C., Burgess J. G., Sode K. Gene transfer in magnetic bacteria: transposon mutagenesis and cloning of genomic DNA fragments required for magnetosome synthesis. J Bacteriol. 1992 May;174(9):2748–2753. doi: 10.1128/jb.174.9.2748-2753.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mindich L., Cohen J., Weisburd M. Isolation of nonsense suppressor mutants in Pseudomonas. J Bacteriol. 1976 Apr;126(1):177–182. doi: 10.1128/jb.126.1.177-182.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Olsen R. H., Siak J. S., Gray R. H. Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage. J Virol. 1974 Sep;14(3):689–699. doi: 10.1128/jvi.14.3.689-699.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Olsen R. H., Thomas D. D. Characteristics and purification of PRR1, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid. J Virol. 1973 Dec;12(6):1560–1567. doi: 10.1128/jvi.12.6.1560-1567.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Palombo E. A., Yusoff K., Stanisich V. A., Krishnapillai V., Willetts N. S. Cloning and genetic analysis of tra cistrons of the Tra 2/Tra 3 region of plasmid RP1. Plasmid. 1989 Jul;22(1):59–69. doi: 10.1016/0147-619x(89)90036-x. [DOI] [PubMed] [Google Scholar]
  24. Romantschuk M., Bamford D. H. The causal agent of halo blight in bean, Pseudomonas syringae pv. phaseolicola, attaches to stomata via its pili. Microb Pathog. 1986 Apr;1(2):139–148. doi: 10.1016/0882-4010(86)90016-1. [DOI] [PubMed] [Google Scholar]
  25. Salas M. Protein-priming of DNA replication. Annu Rev Biochem. 1991;60:39–71. doi: 10.1146/annurev.bi.60.070191.000351. [DOI] [PubMed] [Google Scholar]
  26. Savilahti H., Bamford D. H. Linear DNA replication: inverted terminal repeats of five closely related Escherichia coli bacteriophages. Gene. 1986;49(2):199–205. doi: 10.1016/0378-1119(86)90280-5. [DOI] [PubMed] [Google Scholar]
  27. Savilahti H., Caldentey J., Lundström K., Syväoja J. E., Bamford D. H. Overexpression, purification, and characterization of Escherichia coli bacteriophage PRD1 DNA polymerase. In vitro synthesis of full-length PRD1 DNA with purified proteins. J Biol Chem. 1991 Oct 5;266(28):18737–18744. [PubMed] [Google Scholar]
  28. Thomas C. M., Smith C. A. Incompatibility group P plasmids: genetics, evolution, and use in genetic manipulation. Annu Rev Microbiol. 1987;41:77–101. doi: 10.1146/annurev.mi.41.100187.000453. [DOI] [PubMed] [Google Scholar]
  29. Waters V. L., Strack B., Pansegrau W., Lanka E., Guiney D. G. Mutational analysis of essential IncP alpha plasmid transfer genes traF and traG and involvement of traF in phage sensitivity. J Bacteriol. 1992 Oct;174(20):6666–6673. doi: 10.1128/jb.174.20.6666-6673.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wilkinson R. G., Gemski P., Jr, Stocker B. A. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. J Gen Microbiol. 1972 May;70(3):527–554. doi: 10.1099/00221287-70-3-527. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES