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. 1971 Sep;64(3):559–574.

Ultrastructural Changes in the Alveolar Epithelium in Response to Freund's Adjuvant

Charles S Faulkner, John R Esterly
PMCID: PMC2047624  PMID: 5133517

Abstract

Serial examination of the lungs of neonatal and adult rabbits treated with Freund's adjuvant demonstrated in addition to the rapid development of interstitial granulomata, marked alterations in the alveolar epithelial cells. Granular pneumonocytes became numerous and were often found adjacent to one another within a single alveolus. Their characteristic lamellar inclusions were increased in size and number, and there was often partial loss of the luminal microvilli. The membranous pneumonocytes were locally thickened, had an irregular luminal border, and on occasion, contained lamellar-like inclusions. These observations indicate that both types of epithelial cell participate in the pulmonary reaction to adjuvant, and that adjuvant-induced changes may be a useful model for studying the dynamics of the response of pulmonary epithelial cells to injury.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balis J. U., Delivoria M., Conen P. E. Maturation of postnatal human lung and the idiopathic respiratory distress syndrome. Lab Invest. 1966 Mar;15(3):530–546. [PubMed] [Google Scholar]
  2. Brewer D. B., Heath D., Asquith P. Electron microscopy of desquamative interstitial pneumonia. J Pathol. 1969 Feb;97(2):317–323. doi: 10.1002/path.1710970217. [DOI] [PubMed] [Google Scholar]
  3. Brooks R. E. Pulmonary adenoma of strain A mice: an electron microscopic study. J Natl Cancer Inst. 1968 Sep;41(3):719–742. [PubMed] [Google Scholar]
  4. Galindo B., Imaeda T. Cellular response to Freund's adjuvant in the rabbit lung. An electron microscope study. Lab Invest. 1966 Nov;15(11):1659–1681. [PubMed] [Google Scholar]
  5. Goldenberg V. E., Buckingham S., Sommers S. C. Pulmonary alveolar lesions in vagotomized rats. Lab Invest. 1967 May;16(5):693–705. [PubMed] [Google Scholar]
  6. Hackett R. L., Sunderman F. W., Jr Pulmonary alveolar reaction to nickel carbonyl: ultrastructural and histochemical studies. Arch Environ Health. 1968 Mar;16(3):349–362. doi: 10.1080/00039896.1968.10665070. [DOI] [PubMed] [Google Scholar]
  7. KARRER H. E. The ultrastructure of mouse lung; general architecture of capillary and alveolar walls. J Biophys Biochem Cytol. 1956 May 25;2(3):241–252. doi: 10.1083/jcb.2.3.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kapanci Y., Weibel E. R., Kaplan H. P., Robinson F. R. Pathogenesis and reversibility of the pulmonary lesions of oxygen toxicity in monkeys. II. Ultrastructural and morphometric studies. Lab Invest. 1969 Jan;20(1):101–118. [PubMed] [Google Scholar]
  9. Kikkawa Y., Motoyama E. K., Cook C. D. The ultrastructure of the lungs of lambs. The relation of osmiophilic inclusions and alveolar lining layer to fetal maturation and experimentally produced respiratory distress. Am J Pathol. 1965 Nov;47(5):877–903. [PMC free article] [PubMed] [Google Scholar]
  10. Kuhn C., Györkey F., Levine B. E., Ramirez-Rivera J. Pulmonary alveolar proteinosis. A study using enzyme histochemistry, electron microscopy, and surface tension measurement. Lab Invest. 1966 Feb;15(2):492–509. [PubMed] [Google Scholar]
  11. LAUFER A., TAL C., BEHAR A. J. Effect of adjuvant (Freund's type) and its components on the organs of various animal species; a comparative study. Br J Exp Pathol. 1959 Feb;40(1):1–7. [PMC free article] [PubMed] [Google Scholar]
  12. LOW F. N., SAMPAIO M. M. The pulmonary alveolar epithelium as an entodermal derivative. Anat Rec. 1957 Jan;127(1):51–63. doi: 10.1002/ar.1091270106. [DOI] [PubMed] [Google Scholar]
  13. LOW F. N. The pulmonary alveolar epithelium of laboratory mammals and man. Anat Rec. 1953 Oct;117(2):241–263. doi: 10.1002/ar.1091170208. [DOI] [PubMed] [Google Scholar]
  14. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Leroy E. P., Liebner E. J., Jensik R. J. The ultrastructure of canine alveoli after supervoltage irradiation of the thorax. I. Lesions of the latent period. Lab Invest. 1966 Oct;15(10):1544–1558. [PubMed] [Google Scholar]
  16. Leroy E. P. The blood-air barrier in desquamative interstitial pneumonia (D.I.P.). Virchows Arch A Pathol Pathol Anat. 1969;348(2):117–130. doi: 10.1007/BF00544319. [DOI] [PubMed] [Google Scholar]
  17. MOORE R. D., SCHOENBERG M. D. ALVEOLAR LINING CELLS AND PULMONARY RETICULOENDOTHELIAL SYSTEM OF THE RABBIT. Am J Pathol. 1964 Dec;45:991–1006. [PMC free article] [PubMed] [Google Scholar]
  18. MOORE R. D., SCHOENBERG M. D. THE RESPONSE OF THE HISTIOCYTES AND MACROPHAGES IN THE LUNGS OF RABBITS INJECTED WITH FREUND'S ADJUVANT. Br J Exp Pathol. 1964 Oct;45:488–497. [PMC free article] [PubMed] [Google Scholar]
  19. MYRVIK Q., LEAKE E. S., FARISS B. Studies on pulmonary alveolar macrophages from the normal rabbit: a technique to procure them in a high state of purity. J Immunol. 1961 Feb;86:128–132. [PubMed] [Google Scholar]
  20. Meyrick B., Reid L. The alveolar brush cell in rat lung--a third pneumonocyte. J Ultrastruct Res. 1968 Apr;23(1):71–80. doi: 10.1016/s0022-5320(68)80032-2. [DOI] [PubMed] [Google Scholar]
  21. Pinkett M. O., Cowdrey C. R., Nowell P. C. Mixed hematopoietic and pulmonary origin of 'alveolar macrophages' as demonstrated by chromosome markers. Am J Pathol. 1966 May;48(5):859–867. [PMC free article] [PubMed] [Google Scholar]
  22. Rosenbaum R. M., Wittner M., Lenger M. Mitochondrial and other ultrastructural changes in great alveolar cells of oxygen-adapted and poisoned rats. Lab Invest. 1969 Jun;20(6):516–528. [PubMed] [Google Scholar]
  23. SCHAEFER K. E., AVERY M. E., BENSCH K. TIME COURSE OF CHANGES IN SURFACE TENSION AND MORPHOLOGY OF ALVEOLAR EPITHELIAL CELLS IN CO2-INDUCED HYALINE MEMBRANE DISEASE. J Clin Invest. 1964 Nov;43:2080–2093. doi: 10.1172/JCI105082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. STEINER J. W., LANGER B., SCHATZ D. L. The local and systemic effects of Freund's adjuvant and its fractions. Arch Pathol. 1960 Oct;70:424–434. [PubMed] [Google Scholar]
  25. SVOBODA D. J. Ultrastructure of pulmonary adenomas in mice. Cancer Res. 1962 Nov;22:1197–1201. [PubMed] [Google Scholar]
  26. Sakashita I., Miyoshi M., Hoshino K., Matsukawa T., Asano K. Pulmonary alveolar lesions following open heart surgery. An electron microscopic study. Jpn Heart J. 1968 Sep;9(5):441–452. doi: 10.1536/ihj.9.441. [DOI] [PubMed] [Google Scholar]
  27. Shortland J. R., Darke C. S., Crane W. A. Electron microscopy of desquamative interstitial pneumonia. Thorax. 1969 Mar;24(2):192–208. doi: 10.1136/thx.24.2.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Virolainen M. Hematopoietic origin of macrophages as studied by chromosome markers in mice. J Exp Med. 1968 May 1;127(5):943–952. doi: 10.1084/jem.127.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. WANSTRUP J., CHRISTENSEN H. E. GRANULOMATOUS LESIONS IN MICE PRODUCED BY FREUND'S ADJUVANT; MORPHOGENESIS AND PHASIC DEVELOPMENT. Acta Pathol Microbiol Scand. 1965;63:340–354. doi: 10.1111/apm.1965.63.3.340. [DOI] [PubMed] [Google Scholar]
  30. YOUMANS G. P., YOUMANS A. S. AN ACUTE PULMONARY GRANULOMATOUS RESPONSE IN MICE PRODUCED BY MYCOBACTERIAL CELLS AND ITS RELATION TO INCREASED RESISTANCE AND INCREASED SUSCEPTIBILITY TO EXPERIMENTAL TUBERCULOUS INFECTION. J Infect Dis. 1964 Apr;114:135–151. doi: 10.1093/infdis/114.2.135. [DOI] [PubMed] [Google Scholar]

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