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. 1993 Jun;175(12):3897–3899. doi: 10.1128/jb.175.12.3897-3899.1993

The Salmonella typhimurium uracil-sensitive mutation use is in argU and encodes a minor arginine tRNA.

C D Lu 1, A T Abdelal 1
PMCID: PMC204807  PMID: 8509342

Abstract

The use gene of Salmonella typhimurium was previously identified by a mutation conferring sensitivity to uracil in glucose minimal medium. The use gene was cloned and identified as an allele of argU encoding a tRNA for a minor arginine codon (CGG). The uracil-sensitive phenotype was shown to result from a base substitution in the anticodon stem of this tRNA.

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Selected References

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  1. Bossi L. The hisR locus of Salmonella: nucleotide sequence and expression. Mol Gen Genet. 1983;192(1-2):163–170. doi: 10.1007/BF00327662. [DOI] [PubMed] [Google Scholar]
  2. Bouvier J., Patte J. C., Stragier P. Multiple regulatory signals in the control region of the Escherichia coli carAB operon. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4139–4143. doi: 10.1073/pnas.81.13.4139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bussey L. B., Ingraham J. L. A regulatory gene (use) affecting the expression of pyrA and certain other pyrimidine genes. J Bacteriol. 1982 Jul;151(1):144–152. doi: 10.1128/jb.151.1.144-152.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bussey L. B., Ingraham J. L. Isolation and mapping of a uracil-sensitive mutant of Salmonella typhimurium. Mol Gen Genet. 1982;185(3):513–514. doi: 10.1007/BF00334151. [DOI] [PubMed] [Google Scholar]
  5. Chye M. L., Pittard J. Transcription control of the aroP gene in Escherichia coli K-12: analysis of operator mutants. J Bacteriol. 1987 Jan;169(1):386–393. doi: 10.1128/jb.169.1.386-393.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Daniels D. L., Plunkett G., 3rd, Burland V., Blattner F. R. Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science. 1992 Aug 7;257(5071):771–778. doi: 10.1126/science.1379743. [DOI] [PubMed] [Google Scholar]
  7. Garcia G. M., Mar P. K., Mullin D. A., Walker J. R., Prather N. E. The E. coli dnaY gene encodes an arginine transfer RNA. Cell. 1986 May 9;45(3):453–459. doi: 10.1016/0092-8674(86)90331-4. [DOI] [PubMed] [Google Scholar]
  8. Hüttenhofer A., Weiss-Brummer B., Dirheimer G., Martin R. P. A novel type of + 1 frameshift suppressor: a base substitution in the anticodon stem of a yeast mitochondrial serine-tRNA causes frameshift suppression. EMBO J. 1990 Feb;9(2):551–558. doi: 10.1002/j.1460-2075.1990.tb08142.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kilstrup M., Lu C. D., Abdelal A., Neuhard J. Nucleotide sequence of the carA gene and regulation of the carAB operon in Salmonella typhimurium. Eur J Biochem. 1988 Sep 15;176(2):421–429. doi: 10.1111/j.1432-1033.1988.tb14299.x. [DOI] [PubMed] [Google Scholar]
  10. Komine Y., Adachi T., Inokuchi H., Ozeki H. Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12. J Mol Biol. 1990 Apr 20;212(4):579–598. doi: 10.1016/0022-2836(90)90224-A. [DOI] [PubMed] [Google Scholar]
  11. Lu C. D., Houghton J. E., Abdelal A. T. Characterization of the arginine repressor from Salmonella typhimurium and its interactions with the carAB operator. J Mol Biol. 1992 May 5;225(1):11–24. doi: 10.1016/0022-2836(92)91022-h. [DOI] [PubMed] [Google Scholar]
  12. Lu C. D., Kilstrup M., Neuhard J., Abdelal A. Pyrimidine regulation of tandem promoters for carAB in Salmonella typhimurium. J Bacteriol. 1989 Oct;171(10):5436–5442. doi: 10.1128/jb.171.10.5436-5442.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sanderson K. E., Roth J. R. Linkage map of Salmonella typhimurium, edition VII. Microbiol Rev. 1988 Dec;52(4):485–532. doi: 10.1128/mr.52.4.485-532.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spanjaard R. A., Chen K., Walker J. R., van Duin J. Frameshift suppression at tandem AGA and AGG codons by cloned tRNA genes: assigning a codon to argU tRNA and T4 tRNA(Arg). Nucleic Acids Res. 1990 Sep 11;18(17):5031–5036. doi: 10.1093/nar/18.17.5031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stoker N. G., Fairweather N. F., Spratt B. G. Versatile low-copy-number plasmid vectors for cloning in Escherichia coli. Gene. 1982 Jun;18(3):335–341. doi: 10.1016/0378-1119(82)90172-x. [DOI] [PubMed] [Google Scholar]
  16. Tamura F., Nishimura S., Ohki M. The E. coli divE mutation, which differentially inhibits synthesis of certain proteins, is in tRNASer1. EMBO J. 1984 May;3(5):1103–1107. doi: 10.1002/j.1460-2075.1984.tb01936.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Travers A. A. Promoter sequence for stringent control of bacterial ribonucleic acid synthesis. J Bacteriol. 1980 Feb;141(2):973–976. doi: 10.1128/jb.141.2.973-976.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tsui H. C., Arps P. J., Connolly D. M., Winkler M. E. Absence of hisT-mediated tRNA pseudouridylation results in a uracil requirement that interferes with Escherichia coli K-12 cell division. J Bacteriol. 1991 Nov;173(22):7395–7400. doi: 10.1128/jb.173.22.7395-7400.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  20. Wong S. C., Abdelal A. T. Unorthodox expression of an enzyme: evidence for an untranslated region within carA from Pseudomonas aeruginosa. J Bacteriol. 1990 Feb;172(2):630–642. doi: 10.1128/jb.172.2.630-642.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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