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. 1993 Aug;175(15):4917–4921. doi: 10.1128/jb.175.15.4917-4921.1993

Menaquinone (vitamin K2) biosynthesis: cloning, nucleotide sequence, and expression of the menC gene from Escherichia coli.

V Sharma 1, R Meganathan 1, M E Hudspeth 1
PMCID: PMC204947  PMID: 8335646

Abstract

The benzenoid aromatic compound o-succinylbenzoic acid is formed by dehydration of the prearomatic compound 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid by the enzyme o-succinylbenzoate synthase, encoded by the menC gene. A 1.3-kb PstI-PvuII fragment was found to complement the menC mutation. The complete nucleotide sequence of this fragment revealed a single open reading frame of 954 bp capable of encoding a 35-kDa protein. A consensus sequence for a ribosomal binding site but no promoter consensus sequences were found. However, the first base of the initiating codon of this open reading frame overlaps the upstream menB gene termination codon, suggesting an operon-like organization for these genes. Consistent with this suggestion, the menB promoter can initiate transcription of the menC gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bentley R., Meganathan R. Biosynthesis of vitamin K (menaquinone) in bacteria. Microbiol Rev. 1982 Sep;46(3):241–280. doi: 10.1128/mr.46.3.241-280.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Dansette P., Azerad R. A new intermediate in naphthoquinone and menaquinone biosynthesis. Biochem Biophys Res Commun. 1970 Sep 10;40(5):1090–1095. doi: 10.1016/0006-291x(70)90906-x. [DOI] [PubMed] [Google Scholar]
  5. Emmons G. T., Campbell I. M., Bentley R. Vitamin K (menaquinone) biosynthesis in bacteria: purification and probable structure of an intermediate prior to o-succinylbenzoate. Biochem Biophys Res Commun. 1985 Sep 16;131(2):956–960. doi: 10.1016/0006-291x(85)91332-4. [DOI] [PubMed] [Google Scholar]
  6. Meganathan R., Bentley R. Biosynthesis of o-succinylbenzoic acid in a men- Escherichia coli mutant requires decarboxylation of L-glutamate at the C-1 position. Biochemistry. 1981 Sep 1;20(18):5336–5340. doi: 10.1021/bi00521a038. [DOI] [PubMed] [Google Scholar]
  7. Meganathan R., Bentley R. Thiamine pyrophosphate requirement for o-succinylbenzoic acid synthesis in Escherichia coli and evidence for an intermediate. J Bacteriol. 1983 Feb;153(2):739–746. doi: 10.1128/jb.153.2.739-746.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Meganathan R. Enzymes from Escherichia coli synthesize o-succinylbenzoic acid, an intermediate in menaquinone (vitamin K2) biosynthesis. J Biol Chem. 1981 Sep 25;256(18):9386–9388. [PubMed] [Google Scholar]
  9. Palaniappan C., Sharma V., Hudspeth M. E., Meganathan R. Menaquinone (vitamin K2) biosynthesis: evidence that the Escherichia coli menD gene encodes both 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid synthase and alpha-ketoglutarate decarboxylase activities. J Bacteriol. 1992 Dec;174(24):8111–8118. doi: 10.1128/jb.174.24.8111-8118.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Popp J. L., Berliner C., Bentley R. Vitamin K (menaquinone) biosynthesis in bacteria: high-performance liquid chromatographic assay of the overall synthesis of o-succinylbenzoic acid and of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid synthase. Anal Biochem. 1989 May 1;178(2):306–310. doi: 10.1016/0003-2697(89)90643-x. [DOI] [PubMed] [Google Scholar]
  11. Popp J. L. Sequence and overexpression of the menD gene from Escherichia coli. J Bacteriol. 1989 Aug;171(8):4349–4354. doi: 10.1128/jb.171.8.4349-4354.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sharma V., Suvarna K., Meganathan R., Hudspeth M. E. Menaquinone (vitamin K2) biosynthesis: nucleotide sequence and expression of the menB gene from Escherichia coli. J Bacteriol. 1992 Aug;174(15):5057–5062. doi: 10.1128/jb.174.15.5057-5062.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tummuru M. K., Brickman T. J., McIntosh M. A. The in vitro conversion of chorismate to isochorismate catalyzed by the Escherichia coli entC gene product. Evidence that EntA does not contribute to isochorismate synthase activity. J Biol Chem. 1989 Dec 5;264(34):20547–20551. [PubMed] [Google Scholar]

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