Abstract
Bacillus subtilis 5:7, a derivative of macrofiber-producing strain FJ7, carries the lacZ reporter gene within Tn917 at an unknown location in the host genome. Expression of the host gene carrying lacZ within colonies of 5:7 was observed by examining growth under different conditions in the presence of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). At a high plating density small colonies arose that expressed the host gene early and throughout the colony, whereas at a low density large colonies were produced that expressed the host gene late in development and only in cells forming a ring pattern close to the colony periphery. A highly regulated spatial and temporal gene expression pattern was observed in growth from cross-streaks, suggesting that gene expression is responsive to concentration gradient fields established by neighboring growth. Colonies cultured on agar blocks revealed that expression was governed by depletion of a medium component and also by the geometry of the substrate upon which the colonies grew. At least three factors influenced the control of expression: (i) the concentration of a diffusible component of the medium exhausted by cell growth, (ii) a spatial-temporal factor related to growth within the colony, and (iii) the geometry of the growth substrate.
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- Belas R., Simon M., Silverman M. Regulation of lateral flagella gene transcription in Vibrio parahaemolyticus. J Bacteriol. 1986 Jul;167(1):210–218. doi: 10.1128/jb.167.1.210-218.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fein J. E., Rogers H. J. Autolytic enzyme-deficient mutants of Bacillus subtilis 168. J Bacteriol. 1976 Sep;127(3):1427–1442. doi: 10.1128/jb.127.3.1427-1442.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fisher S. H., Sonenshein A. L. Control of carbon and nitrogen metabolism in Bacillus subtilis. Annu Rev Microbiol. 1991;45:107–135. doi: 10.1146/annurev.mi.45.100191.000543. [DOI] [PubMed] [Google Scholar]
- Gomer R. H., Datta S., Mehdy M., Crowley T., Sivertsen A., Nellen W., Reymond C., Mann S., Firtel R. A. Regulation of cell-type-specific gene expression in Dictyostelium. Cold Spring Harb Symp Quant Biol. 1985;50:801–812. doi: 10.1101/sqb.1985.050.01.098. [DOI] [PubMed] [Google Scholar]
- Gottlieb D. I. GABAergic neurons. Sci Am. 1988 Feb;258(2):82–89. doi: 10.1038/scientificamerican0288-82. [DOI] [PubMed] [Google Scholar]
- Gurdon J. B. Molecular biology of development. Introductory comments. Cold Spring Harb Symp Quant Biol. 1985;50:1–10. doi: 10.1101/sqb.1985.050.01.003. [DOI] [PubMed] [Google Scholar]
- Kim S. K., Kaiser D. Cell alignment required in differentiation of Myxococcus xanthus. Science. 1990 Aug 24;249(4971):926–928. doi: 10.1126/science.2118274. [DOI] [PubMed] [Google Scholar]
- Mendelson N. H. Bacterial macrofibres: the morphogenesis of complex multicellular bacterial forms. Sci Prog. 1990;74(296 Pt 4):425–441. [PubMed] [Google Scholar]
- Shapiro J. A., Higgins N. P. Variation of beta-galactosidase expression from Mudlac elements during the development of Escherichia coli colonies. Ann Inst Pasteur Microbiol. 1988 Jan-Feb;139(1):79–103. doi: 10.1016/0769-2609(88)90098-1. [DOI] [PubMed] [Google Scholar]
- Shapiro J. A. Organization of developing Escherichia coli colonies viewed by scanning electron microscopy. J Bacteriol. 1987 Jan;169(1):142–156. doi: 10.1128/jb.169.1.142-156.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomich P. K., An F. Y., Clewell D. B. Properties of erythromycin-inducible transposon Tn917 in Streptococcus faecalis. J Bacteriol. 1980 Mar;141(3):1366–1374. doi: 10.1128/jb.141.3.1366-1374.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]