Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Feb;176(3):935–937. doi: 10.1128/jb.176.3.935-937.1994

Expression of the Bacillus subtilis spoIVCA gene, which encodes a site-specific recombinase, depends on the spoIIGB product.

T Sato 1, K Harada 1, Y Ohta 1, Y Kobayashi 1
PMCID: PMC205134  PMID: 8300549

Abstract

The Bacillus subtilis spoIVCA gene encodes a site-specific recombinase which creates a sigK gene by DNA rearrangement. We have determined the transcription initiation point of the spoIVCA gene and found that (i) the spoIVCA promoter contains sequences which are similar to -10 and -35 regions of promoters recognized by sigma E and (ii) mutation of spoIIGB, which encodes pro-sigma E, blocked the expression of spoIVCA.

Full text

PDF
935

Images in this article

936Image
on p.936

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beall B., Driks A., Losick R., Moran C. P., Jr Cloning and characterization of a gene required for assembly of the Bacillus subtilis spore coat. J Bacteriol. 1993 Mar;175(6):1705–1716. doi: 10.1128/jb.175.6.1705-1716.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Errington J., Rong S., Rosenkrantz M. S., Sonenshein A. L. Transcriptional regulation and structure of the Bacillus subtilis sporulation locus spoIIIC. J Bacteriol. 1988 Mar;170(3):1162–1167. doi: 10.1128/jb.170.3.1162-1167.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ireton K., Grossman A. D. Interactions among mutations that cause altered timing of gene expression during sporulation in Bacillus subtilis. J Bacteriol. 1992 May;174(10):3185–3195. doi: 10.1128/jb.174.10.3185-3195.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kunkel B., Losick R., Stragier P. The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene. Genes Dev. 1990 Apr;4(4):525–535. doi: 10.1101/gad.4.4.525. [DOI] [PubMed] [Google Scholar]
  5. Kunkel B., Sandman K., Panzer S., Youngman P., Losick R. The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression. J Bacteriol. 1988 Aug;170(8):3513–3522. doi: 10.1128/jb.170.8.3513-3522.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Masuda E. S., Anaguchi H., Yamada K., Kobayashi Y. Two developmental genes encoding sigma factor homologs are arranged in tandem in Bacillus subtilis. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7637–7641. doi: 10.1073/pnas.85.20.7637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Popham D. L., Stragier P. Binding of the Bacillus subtilis spoIVCA product to the recombination sites of the element interrupting the sigma K-encoding gene. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5991–5995. doi: 10.1073/pnas.89.13.5991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sato T., Samori Y., Kobayashi Y. The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a protein homologous to a site-specific recombinase. J Bacteriol. 1990 Feb;172(2):1092–1098. doi: 10.1128/jb.172.2.1092-1098.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schaeffer P., Millet J., Aubert J. P. Catabolic repression of bacterial sporulation. Proc Natl Acad Sci U S A. 1965 Sep;54(3):704–711. doi: 10.1073/pnas.54.3.704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith K., Youngman P. Evidence that the spoIIM gene of Bacillus subtilis is transcribed by RNA polymerase associated with sigma E. J Bacteriol. 1993 Jun;175(11):3618–3627. doi: 10.1128/jb.175.11.3618-3627.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stragier P., Kunkel B., Kroos L., Losick R. Chromosomal rearrangement generating a composite gene for a developmental transcription factor. Science. 1989 Jan 27;243(4890):507–512. doi: 10.1126/science.2536191. [DOI] [PubMed] [Google Scholar]
  13. Theeragool G., Miyao A., Yamada K., Sato T., Kobayashi Y. In vivo expression of the Bacillus subtilis spoVE gene. J Bacteriol. 1993 Jul;175(13):4071–4080. doi: 10.1128/jb.175.13.4071-4080.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES