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. 1994 Feb;176(3):935–937. doi: 10.1128/jb.176.3.935-937.1994

Expression of the Bacillus subtilis spoIVCA gene, which encodes a site-specific recombinase, depends on the spoIIGB product.

T Sato 1, K Harada 1, Y Ohta 1, Y Kobayashi 1
PMCID: PMC205134  PMID: 8300549

Abstract

The Bacillus subtilis spoIVCA gene encodes a site-specific recombinase which creates a sigK gene by DNA rearrangement. We have determined the transcription initiation point of the spoIVCA gene and found that (i) the spoIVCA promoter contains sequences which are similar to -10 and -35 regions of promoters recognized by sigma E and (ii) mutation of spoIIGB, which encodes pro-sigma E, blocked the expression of spoIVCA.

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Selected References

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  1. Beall B., Driks A., Losick R., Moran C. P., Jr Cloning and characterization of a gene required for assembly of the Bacillus subtilis spore coat. J Bacteriol. 1993 Mar;175(6):1705–1716. doi: 10.1128/jb.175.6.1705-1716.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Errington J., Rong S., Rosenkrantz M. S., Sonenshein A. L. Transcriptional regulation and structure of the Bacillus subtilis sporulation locus spoIIIC. J Bacteriol. 1988 Mar;170(3):1162–1167. doi: 10.1128/jb.170.3.1162-1167.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ireton K., Grossman A. D. Interactions among mutations that cause altered timing of gene expression during sporulation in Bacillus subtilis. J Bacteriol. 1992 May;174(10):3185–3195. doi: 10.1128/jb.174.10.3185-3195.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kunkel B., Losick R., Stragier P. The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene. Genes Dev. 1990 Apr;4(4):525–535. doi: 10.1101/gad.4.4.525. [DOI] [PubMed] [Google Scholar]
  5. Kunkel B., Sandman K., Panzer S., Youngman P., Losick R. The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression. J Bacteriol. 1988 Aug;170(8):3513–3522. doi: 10.1128/jb.170.8.3513-3522.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Masuda E. S., Anaguchi H., Yamada K., Kobayashi Y. Two developmental genes encoding sigma factor homologs are arranged in tandem in Bacillus subtilis. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7637–7641. doi: 10.1073/pnas.85.20.7637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Popham D. L., Stragier P. Binding of the Bacillus subtilis spoIVCA product to the recombination sites of the element interrupting the sigma K-encoding gene. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5991–5995. doi: 10.1073/pnas.89.13.5991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sato T., Samori Y., Kobayashi Y. The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a protein homologous to a site-specific recombinase. J Bacteriol. 1990 Feb;172(2):1092–1098. doi: 10.1128/jb.172.2.1092-1098.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schaeffer P., Millet J., Aubert J. P. Catabolic repression of bacterial sporulation. Proc Natl Acad Sci U S A. 1965 Sep;54(3):704–711. doi: 10.1073/pnas.54.3.704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith K., Youngman P. Evidence that the spoIIM gene of Bacillus subtilis is transcribed by RNA polymerase associated with sigma E. J Bacteriol. 1993 Jun;175(11):3618–3627. doi: 10.1128/jb.175.11.3618-3627.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stragier P., Kunkel B., Kroos L., Losick R. Chromosomal rearrangement generating a composite gene for a developmental transcription factor. Science. 1989 Jan 27;243(4890):507–512. doi: 10.1126/science.2536191. [DOI] [PubMed] [Google Scholar]
  13. Theeragool G., Miyao A., Yamada K., Sato T., Kobayashi Y. In vivo expression of the Bacillus subtilis spoVE gene. J Bacteriol. 1993 Jul;175(13):4071–4080. doi: 10.1128/jb.175.13.4071-4080.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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