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. 1994 Feb;176(4):948–952. doi: 10.1128/jb.176.4.948-952.1994

Translocation of an outer membrane protein into prey cytoplasmic membranes by bdellovibrios.

J J Tudor 1, M A Karp 1
PMCID: PMC205143  PMID: 8106336

Abstract

Within minutes of Bdellovibrio bacteriovorus attack on prey cells, such as Escherichia coli, the cytoplasmic membrane of the prey is altered. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified invaded prey cell (bdelloplast) membranes revealed the appearance of a noncytoplasmic membrane protein. This protein is not observed in preparations of noninvaded E. coli membranes and migrates in a manner similar to that of E. coli OmpF. Isoelectric focusing and two-dimensional gel electrophoresis of bdelloplast cytoplasmic membrane preparations also revealed the presence of a protein with electrophoretic properties similar to those of OmpF and the major Bdellovibrio outer membrane proteins. The protein appears in cytoplasmic membrane preparations within minutes of attack and persists throughout most of the intraperiplasmic developmental cycle. The appearance of this protein is consistent with our hypothesis that bdellovibrios translocate a pore protein into the bdelloplast cytoplasmic membrane to kill their prey and to gain access to the cytoplasmic contents for growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abram D., Castro e Melo J., Chou D. Penetration of Bdellovibrio bacteriovorus into host cells. J Bacteriol. 1974 May;118(2):663–680. doi: 10.1128/jb.118.2.663-680.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blake M. S., Gotschlich E. C. Gonococcal membrane proteins: speculation on their role in pathogenesis. Prog Allergy. 1983;33:298–313. [PubMed] [Google Scholar]
  3. Burnham J. C., Hashimoto T., Conti S. F. Electron microscopic observations on the penetration of Bdellovibrio bacteriovorus into gram-negative bacterial hosts. J Bacteriol. 1968 Oct;96(4):1366–1381. doi: 10.1128/jb.96.4.1366-1381.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cover W. H., Martinez R. J., Rittenberg S. C. Permeability of the boundary layers of Bdellovibrio bacteriovorus 109J and its bdelloplasts to small hydrophilic molecules. J Bacteriol. 1984 Feb;157(2):385–390. doi: 10.1128/jb.157.2.385-390.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Diedrich D. L., Duran C. P., Conti S. F. Acquisition of Escherichia coli outer membrane proteins by Bdellovibrio sp. strain 109D. J Bacteriol. 1984 Jul;159(1):329–334. doi: 10.1128/jb.159.1.329-334.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diedrich D. L., Summers A. O., Schnaitman C. A. Outer membrane proteins of Escherichia coli. V. Evidence that protein 1 and bacteriophage-directed protein 2 are different polypeptides. J Bacteriol. 1977 Aug;131(2):598–607. doi: 10.1128/jb.131.2.598-607.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Galdiero F. Membrane damage and incorporation of Escherichia coli components into Bdellovibrio bacteriovorus. Zentralbl Bakteriol Orig A. 1975;230(2):203–209. [PubMed] [Google Scholar]
  8. Guerrini F., Romano V., Valenzi M., Di Giulio M., Mupo M. R., Sacco M. Molecular parasitism in the Escherichia coli-Bdellovibrio bacteriovorus system: translocation of the matrix protein from the host to the parasite outer membrane. EMBO J. 1982;1(11):1439–1444. doi: 10.1002/j.1460-2075.1982.tb01335.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Lynch E. C., Blake M. S., Gotschlich E. C., Mauro A. Studies of Porins: Spontaneously Transferred from Whole Cells and Reconstituted from Purified Proteins of Neisseria gonorrhoeae and Neisseria meningitidis. Biophys J. 1984 Jan;45(1):104–107. doi: 10.1016/S0006-3495(84)84127-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Matin A., Rittenberg S. C. Kinetics of deoxyribonucleic acid destruction and synthesis during growth of Bdellovibrio bacteriovorus strain 109D on pseudomonas putida and escherichia coli. J Bacteriol. 1972 Sep;111(3):664–673. doi: 10.1128/jb.111.3.664-673.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  13. Rayner J. R., Cover W. H., Martinez R. J., Rittenberg S. C. Bdellovibrio bacteriovorus synthesizes an OmpF-like outer membrane protein during both axenic and intraperiplasmic growth. J Bacteriol. 1985 Aug;163(2):595–599. doi: 10.1128/jb.163.2.595-599.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rittenberg S. C., Shilo M. Early host damage in the infection cycle of Bdellovibrio bacteriovorus. J Bacteriol. 1970 Apr;102(1):149–160. doi: 10.1128/jb.102.1.149-160.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rodriguez M. H., Jungery M. A protein on Plasmodium falciparum-infected erythrocytes functions as a transferrin receptor. 1986 Nov 27-Dec 3Nature. 324(6095):388–391. doi: 10.1038/324388a0. [DOI] [PubMed] [Google Scholar]
  16. Shilo M., Bruff B. Lysis of Gram-negative bacteria by host-independent ectoparasitic Bdellovibrio bacteriovorus isolates. J Gen Microbiol. 1965 Sep;40(3):317–328. doi: 10.1099/00221287-40-3-317. [DOI] [PubMed] [Google Scholar]
  17. Talley B. G., McDade R. L., Jr, Diedrich D. L. Verification of the protein in the outer membrane of Bdellovibrio bacteriovorus as the OmpF protein of its Escherichia coli prey. J Bacteriol. 1987 Feb;169(2):694–698. doi: 10.1128/jb.169.2.694-698.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Thomashow M. F., Rittenberg S. C. Intraperiplasmic growth of Bdellovibrio bacteriovorus 109J: N-deacetylation of Escherichia coli peptidoglycan amino sugars. J Bacteriol. 1978 Sep;135(3):1008–1014. doi: 10.1128/jb.135.3.1008-1014.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tudor J. J., McCann M. P., Acrich I. A. A new model for the penetration of prey cells by bdellovibrios. J Bacteriol. 1990 May;172(5):2421–2426. doi: 10.1128/jb.172.5.2421-2426.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Young J. D., Blake M., Mauro A., Cohn Z. A. Properties of the major outer membrane protein from Neisseria gonorrhoeae incorporated into model lipid membranes. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3831–3835. doi: 10.1073/pnas.80.12.3831. [DOI] [PMC free article] [PubMed] [Google Scholar]

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