Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Feb;176(4):1180–1183. doi: 10.1128/jb.176.4.1180-1183.1994

Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping.

E Tigges 1, F C Minion 1
PMCID: PMC205171  PMID: 8106329

Abstract

A physical chromosomal map of Acholeplasma oculi ISM1499 was constructed by using field inversion gel electrophoresis. To assist in the ordering of the chromosomal fragments, a modified transposon, Tn4001.1064, was constructed. It was also used to rescue mycoplasmal chromosomal sequences adjacent to transposon insertion sites in a one-step cloning procedure. The total size of the A. oculi ISM1499 genome was estimated to be 1,633 kb. The restriction enzyme sites for ApaI, BssHII, EagI, and SmaI were positioned on the map along with several transposon insertion sites.

Full text

PDF
1180

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bautsch W. Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques. Nucleic Acids Res. 1988 Dec 23;16(24):11461–11467. doi: 10.1093/nar/16.24.11461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Byrne M. E., Rouch D. A., Skurray R. A. Nucleotide sequence analysis of IS256 from the Staphylococcus aureus gentamicin-tobramycin-kanamycin-resistance transposon Tn4001. Gene. 1989 Sep 30;81(2):361–367. doi: 10.1016/0378-1119(89)90197-2. [DOI] [PubMed] [Google Scholar]
  3. Heller C., Pohl F. M. A systematic study of field inversion gel electrophoresis. Nucleic Acids Res. 1989 Aug 11;17(15):5989–6003. doi: 10.1093/nar/17.15.5989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Krause D. C., Mawn C. B. Physical analysis and mapping of the Mycoplasma pneumoniae chromosome. J Bacteriol. 1990 Sep;172(9):4790–4797. doi: 10.1128/jb.172.9.4790-4797.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ladefoged S. A., Christiansen G. Physical and genetic mapping of the genomes of five Mycoplasma hominis strains by pulsed-field gel electrophoresis. J Bacteriol. 1992 Apr;174(7):2199–2207. doi: 10.1128/jb.174.7.2199-2207.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mahairas G. G., Jian C., Minion F. C. Development of a cloning system in Mycoplasma pulmonis. Gene. 1990 Sep 1;93(1):61–66. doi: 10.1016/0378-1119(90)90136-f. [DOI] [PubMed] [Google Scholar]
  7. Mahairas G. G., Minion F. C. Random insertion of the gentamicin resistance transposon Tn4001 in Mycoplasma pulmonis. Plasmid. 1989 Jan;21(1):43–47. doi: 10.1016/0147-619x(89)90085-1. [DOI] [PubMed] [Google Scholar]
  8. Mahairas G. G., Minion F. C. Transformation of Mycoplasma pulmonis: demonstration of homologous recombination, introduction of cloned genes, and preliminary description of an integrating shuttle system. J Bacteriol. 1989 Apr;171(4):1775–1780. doi: 10.1128/jb.171.4.1775-1780.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Minion F. C., Jarvill-Taylor K. J., Billings D. E., Tigges E. Membrane-associated nuclease activities in mycoplasmas. J Bacteriol. 1993 Dec;175(24):7842–7847. doi: 10.1128/jb.175.24.7842-7847.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miyata M., Wang L., Fukumura T. Physical mapping of the Mycoplasma capricolum genome. FEMS Microbiol Lett. 1991 Apr 15;63(2-3):329–333. doi: 10.1016/0378-1097(91)90107-l. [DOI] [PubMed] [Google Scholar]
  11. Neimark H. C., Lange C. S. Pulse-field electrophoresis indicates full-length Mycoplasma chromosomes range widely in size. Nucleic Acids Res. 1990 Sep 25;18(18):5443–5448. doi: 10.1093/nar/18.18.5443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nur I., Szyf M., Razin A., Glaser G., Rottem S., Razin S. Procaryotic and eucaryotic traits of DNA methylation in spiroplasmas (mycoplasmas). J Bacteriol. 1985 Oct;164(1):19–24. doi: 10.1128/jb.164.1.19-24.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pyle L. E., Finch L. R. A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci. Nucleic Acids Res. 1988 Jul 11;16(13):6027–6039. doi: 10.1093/nar/16.13.6027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pyle L. E., Finch L. R. Preparation and FIGE separation of infrequent restriction fragments from Mycoplasma mycoides DNA. Nucleic Acids Res. 1988 Mar 25;16(5):2263–2268. doi: 10.1093/nar/16.5.2263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pyle L. E., Taylor T., Finch L. R. Genomic maps of some strains within the Mycoplasma mycoides cluster. J Bacteriol. 1990 Dec;172(12):7265–7268. doi: 10.1128/jb.172.12.7265-7268.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. doi: 10.1128/mr.49.4.419-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rouch D. A., Byrne M. E., Kong Y. C., Skurray R. A. The aacA-aphD gentamicin and kanamycin resistance determinant of Tn4001 from Staphylococcus aureus: expression and nucleotide sequence analysis. J Gen Microbiol. 1987 Nov;133(11):3039–3052. doi: 10.1099/00221287-133-11-3039. [DOI] [PubMed] [Google Scholar]
  18. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991 Jan;173(2):697–703. doi: 10.1128/jb.173.2.697-703.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wenzel R., Herrmann R. Physical mapping of the Mycoplasma pneumoniae genome. Nucleic Acids Res. 1988 Sep 12;16(17):8323–8336. doi: 10.1093/nar/16.17.8323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Whitley J. C., Muto A., Finch L. R. A physical map for Mycoplasma capricolum Cal. kid with loci for all known tRNA species. Nucleic Acids Res. 1991 Jan 25;19(2):399–400. doi: 10.1093/nar/19.2.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ye F., Laigret F., Whitley J. C., Citti C., Finch L. R., Carle P., Renaudin J., Bové J. M. A physical and genetic map of the Spiroplasma citri genome. Nucleic Acids Res. 1992 Apr 11;20(7):1559–1565. doi: 10.1093/nar/20.7.1559. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES