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. 1994 Feb;176(4):1192–1196. doi: 10.1128/jb.176.4.1192-1196.1994

One-ended insertion of IS911.

P Polard 1, L Seroude 1, O Fayet 1, M F Prère 1, M Chandler 1
PMCID: PMC205174  PMID: 8106332

Abstract

An apparently nonreplicative integration reaction mediated by the insertion sequence IS911 has been analyzed. It is shown to involve the right-end inverted repeat (IRR) of the element and sequences in the flanking vector DNA. The flanking sequences appear to behave as a surrogate IS911 end, since integration is greatly reduced when limited similarities with IRR are eliminated by site-directed mutagenesis. Data are presented which suggest that the activity of the IRR junction results from the proximity of the transposase gene and may therefore reflect preferential transposase recognition of IRR in cis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg D. E. Structural requirement for IS50-mediated gene transposition. Proc Natl Acad Sci U S A. 1983 Feb;80(3):792–796. doi: 10.1073/pnas.80.3.792. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Derbyshire K. M., Grindley N. D. Binding of the IS903 transposase to its inverted repeat in vitro. EMBO J. 1992 Sep;11(9):3449–3455. doi: 10.1002/j.1460-2075.1992.tb05424.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Derbyshire K. M., Hwang L., Grindley N. D. Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats. Proc Natl Acad Sci U S A. 1987 Nov;84(22):8049–8053. doi: 10.1073/pnas.84.22.8049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fellay R., Frey J., Krisch H. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene. 1987;52(2-3):147–154. doi: 10.1016/0378-1119(87)90041-2. [DOI] [PubMed] [Google Scholar]
  5. Grindley N. D., Joyce C. M. Analysis of the structure and function of the kanamycin-resistance transposon Tn903. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):125–133. doi: 10.1101/sqb.1981.045.01.021. [DOI] [PubMed] [Google Scholar]
  6. Huisman O., Errada P. R., Signon L., Kleckner N. Mutational analysis of IS10's outside end. EMBO J. 1989 Jul;8(7):2101–2109. doi: 10.1002/j.1460-2075.1989.tb03619.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Isberg R. R., Syvanen M. Replicon fusions promoted by the inverted repeats of Tn5. The right repeat is an insertion sequence. J Mol Biol. 1981 Jul 25;150(1):15–32. doi: 10.1016/0022-2836(81)90322-3. [DOI] [PubMed] [Google Scholar]
  8. Machida Y., Machida C., Ohtsubo H., Ohtsubo E. Factors determining frequency of plasmid cointegration mediated by insertion sequence IS1. Proc Natl Acad Sci U S A. 1982 Jan;79(2):277–281. doi: 10.1073/pnas.79.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Makris J. C., Nordmann P. L., Reznikoff W. S. Mutational analysis of insertion sequence 50 (IS50) and transposon 5 (Tn5) ends. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2224–2228. doi: 10.1073/pnas.85.7.2224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morisato D., Way J. C., Kim H. J., Kleckner N. Tn10 transposase acts preferentially on nearby transposon ends in vivo. Cell. 1983 Mar;32(3):799–807. doi: 10.1016/0092-8674(83)90066-1. [DOI] [PubMed] [Google Scholar]
  11. Polard P., Prère M. F., Chandler M., Fayet O. Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911. J Mol Biol. 1991 Dec 5;222(3):465–477. doi: 10.1016/0022-2836(91)90490-w. [DOI] [PubMed] [Google Scholar]
  12. Polard P., Prère M. F., Fayet O., Chandler M. Transposase-induced excision and circularization of the bacterial insertion sequence IS911. EMBO J. 1992 Dec;11(13):5079–5090. doi: 10.1002/j.1460-2075.1992.tb05615.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Prentki P., Pham M. H., Gamas P., Chandler M., Galas D. J. Artificial transposable elements in the study of the ends of IS1. Gene. 1987;61(1):91–101. doi: 10.1016/0378-1119(87)90368-4. [DOI] [PubMed] [Google Scholar]
  14. Prère M. F., Chandler M., Fayet O. Transposition in Shigella dysenteriae: isolation and analysis of IS911, a new member of the IS3 group of insertion sequences. J Bacteriol. 1990 Jul;172(7):4090–4099. doi: 10.1128/jb.172.7.4090-4099.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Twigg A. J., Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature. 1980 Jan 10;283(5743):216–218. doi: 10.1038/283216a0. [DOI] [PubMed] [Google Scholar]
  16. Zerbib D., Polard P., Escoubas J. M., Galas D., Chandler M. The regulatory role of the IS1-encoded InsA protein in transposition. Mol Microbiol. 1990 Mar;4(3):471–477. doi: 10.1111/j.1365-2958.1990.tb00613.x. [DOI] [PubMed] [Google Scholar]
  17. Zhou C., Abaigar L., Jong A. Y. A protocol for using T7 DNA polymerase in oligonucleotide site-directed mutagenesis. Biotechniques. 1990 May;8(5):503–503. [PubMed] [Google Scholar]

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