Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Mar;176(5):1511–1513. doi: 10.1128/jb.176.5.1511-1513.1994

Aspects of glucose uptake in Saccharomyces cerevisiae.

T Gonçalves 1, M C Loureiro-Dias 1
PMCID: PMC205219  PMID: 8113192

Abstract

A wild-type Saccharomyces cerevisiae strain showed simple saturation kinetics for glucose uptake, with a Km of 4 mM when cells were obtained from exponential growth on glucose, and a similar, single Km of 2 to 8 mM was found under a variety of other growth conditions. Later in growth on glucose, and during ethanol utilization, a second kinetic component was observed, which might reflect either artifacts of membrane alteration or a Km in the molar range.

Full text

PDF
1511

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benito B., Lagunas R. The low-affinity component of Saccharomyces cerevisiae maltose transport is an artifact. J Bacteriol. 1992 May;174(9):3065–3069. doi: 10.1128/jb.174.9.3065-3069.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bisson L. F., Fraenkel D. G. Expression of kinase-dependent glucose uptake in Saccharomyces cerevisiae. J Bacteriol. 1984 Sep;159(3):1013–1017. doi: 10.1128/jb.159.3.1013-1017.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bisson L. F., Fraenkel D. G. Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1730–1734. doi: 10.1073/pnas.80.6.1730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bisson L. F. High-affinity glucose transport in Saccharomyces cerevisiae is under general glucose repression control. J Bacteriol. 1988 Oct;170(10):4838–4845. doi: 10.1128/jb.170.10.4838-4845.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bisson L. F., Neigeborn L., Carlson M., Fraenkel D. G. The SNF3 gene is required for high-affinity glucose transport in Saccharomyces cerevisiae. J Bacteriol. 1987 Apr;169(4):1656–1662. doi: 10.1128/jb.169.4.1656-1662.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Celenza J. L., Marshall-Carlson L., Carlson M. The yeast SNF3 gene encodes a glucose transporter homologous to the mammalian protein. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2130–2134. doi: 10.1073/pnas.85.7.2130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Entian K. D., Zimmermann F. K. New genes involved in carbon catabolite repression and derepression in the yeast Saccharomyces cerevisiae. J Bacteriol. 1982 Sep;151(3):1123–1128. doi: 10.1128/jb.151.3.1123-1128.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fuhrmann G. F., Völker B. Misuse of graphical analysis in nonlinear sugar transport kinetics by Eadie-Hofstee plots. Biochim Biophys Acta. 1993 Jan 18;1145(1):180–182. doi: 10.1016/0005-2736(93)90396-h. [DOI] [PubMed] [Google Scholar]
  9. Gasnier B. Characterization of low- and high-affinity glucose transports in the yeast Kluyveromyces marxianus. Biochim Biophys Acta. 1987 Oct 16;903(3):425–433. doi: 10.1016/0005-2736(87)90049-6. [DOI] [PubMed] [Google Scholar]
  10. Ko C. H., Liang H., Gaber R. F. Roles of multiple glucose transporters in Saccharomyces cerevisiae. Mol Cell Biol. 1993 Jan;13(1):638–648. doi: 10.1128/mcb.13.1.638. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kruckeberg A. L., Bisson L. F. The HXT2 gene of Saccharomyces cerevisiae is required for high-affinity glucose transport. Mol Cell Biol. 1990 Nov;10(11):5903–5913. doi: 10.1128/mcb.10.11.5903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lagunas R. Sugar transport in Saccharomyces cerevisiae. FEMS Microbiol Rev. 1993 Apr;10(3-4):229–242. doi: 10.1016/0378-1097(93)90598-v. [DOI] [PubMed] [Google Scholar]
  13. Lewis D. A., Bisson L. F. The HXT1 gene product of Saccharomyces cerevisiae is a new member of the family of hexose transporters. Mol Cell Biol. 1991 Jul;11(7):3804–3813. doi: 10.1128/mcb.11.7.3804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schüller H. J., Entian K. D. Extragenic suppressors of yeast glucose derepression mutants leading to constitutive synthesis of several glucose-repressible enzymes. J Bacteriol. 1991 Mar;173(6):2045–2052. doi: 10.1128/jb.173.6.2045-2052.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. van Uden N. Transport-limited fermentation and growth of saccharomyces cerevisiae and its competitive inhibition. Arch Mikrobiol. 1967;58(2):155–168. doi: 10.1007/BF00406676. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES