Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Mar;176(6):1756–1760. doi: 10.1128/jb.176.6.1756-1760.1994

Isolation of an R- M+ mutant of Yersinia enterocolitica serotype O:8 and its application in construction of rough mutants utilizing mini-Tn5 derivatives and lipopolysaccharide-specific phage.

L Zhang 1, M Skurnik 1
PMCID: PMC205264  PMID: 8132471

Abstract

A generally applicable procedure was used to isolate a spontaneous restriction-deficient mutant of Yersinia enterocolitica serotype O:8. Transposition frequency in the mutant strain 8081-res was approximately 6.7 x 10(-6) per recipient, while it was practically zero in the wild-type strain 8081-c. Mobilization frequency into 8081-res was 10(5) times higher than that into the wild-type strain. The mutant had lost the ability to express the YenI restriction endonuclease activity present in serotype O:8 strains. This allowed the construction of a transposon library in 8081-res. Insertion mutants with transposons in the genes of the rfa region were selected from this library.

Full text

PDF
1756

Images in this article

1758Image
on p.1758
1759Image
on p.1759

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERTANI G., WEIGLE J. J. Host controlled variation in bacterial viruses. J Bacteriol. 1953 Feb;65(2):113–121. doi: 10.1128/jb.65.2.113-121.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker P. M., Farmer J. J., 3rd New bacteriophage typing system for Yersinia enterocolitica, Yersinia kristensenii, Yersinia frederiksenii, and Yersinia intermedia: correlation with serotyping, biotyping, and antibiotic susceptibility. J Clin Microbiol. 1982 Mar;15(3):491–502. doi: 10.1128/jcm.15.3.491-502.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Butkus V., Klimasauskas S., Kersulyte D., Vaitkevicius D., Lebionka A., Janulaitis A. Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate. Nucleic Acids Res. 1985 Aug 26;13(16):5727–5746. doi: 10.1093/nar/13.16.5727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cerritelli S., Springhorn S. S., Lacks S. A. DpnA, a methylase for single-strand DNA in the Dpn II restriction system, and its biological function. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9223–9227. doi: 10.1073/pnas.86.23.9223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Colson C., Colson A. M., Van Pel A. Chromosomal location of host specificity in Salmonella typhimurium. J Gen Microbiol. 1970 Feb;60(2):265–271. doi: 10.1099/00221287-60-2-265. [DOI] [PubMed] [Google Scholar]
  7. Colson C., Van Pel A. DNA restriction and modification systems in Salmonella. I. SA and SB, two Salmonella typhimurium systems determined by genes with a chromosomal location comparable to that of the Escherichia coli hsd genes. Mol Gen Genet. 1974 Apr 3;129(4):325–337. doi: 10.1007/BF00265696. [DOI] [PubMed] [Google Scholar]
  8. Ehrlich M., Gama-Sosa M. A., Carreira L. H., Ljungdahl L. G., Kuo K. C., Gehrke C. W. DNA methylation in thermophilic bacteria: N4-methylcytosine, 5-methylcytosine, and N6-methyladenine. Nucleic Acids Res. 1985 Feb 25;13(4):1399–1412. doi: 10.1093/nar/13.4.1399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Granfors K., Jalkanen S., von Essen R., Lahesmaa-Rantala R., Isomäki O., Pekkola-Heino K., Merilahti-Palo R., Saario R., Isomäki H., Toivanen A. Yersinia antigens in synovial-fluid cells from patients with reactive arthritis. N Engl J Med. 1989 Jan 26;320(4):216–221. doi: 10.1056/NEJM198901263200404. [DOI] [PubMed] [Google Scholar]
  10. Gromkova R., Goodgal S. H. Biological properties of a Haemophilus influenzae restriction enzyme, Hind I. J Bacteriol. 1976 Aug;127(2):848–854. doi: 10.1128/jb.127.2.848-854.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hadi S. M., Bächi B., Shepherd J. C., Yuan R., Ineichen K., Bickle T. A. DNA recognition and cleavage by the EcoP15 restriction endonuclease. J Mol Biol. 1979 Nov 5;134(3):655–666. doi: 10.1016/0022-2836(79)90372-3. [DOI] [PubMed] [Google Scholar]
  12. Hattman S., Gold E., Plotnik A. Methylation of cytosine residues in DNA controlled by a drug resistance factor (host-induced modification-R factors-N 6 -methyladenine-5-methylcytosine). Proc Natl Acad Sci U S A. 1972 Jan;69(1):187–190. doi: 10.1073/pnas.69.1.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ives C. L., Nathan P. D., Brooks J. E. Regulation of the BamHI restriction-modification system by a small intergenic open reading frame, bamHIC, in both Escherichia coli and Bacillus subtilis. J Bacteriol. 1992 Nov;174(22):7194–7201. doi: 10.1128/jb.174.22.7194-7201.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jansson P. E., Lindberg A. A., Lindberg B., Wollin R. Structural studies on the hexose region of the core in lipopolysaccharides from Enterobacteriaceae. Eur J Biochem. 1981 Apr;115(3):571–577. doi: 10.1111/j.1432-1033.1981.tb06241.x. [DOI] [PubMed] [Google Scholar]
  15. Janulaitis A., Klimasauskas S., Petrusyte M., Butkus V. Cytosine modification in DNA by BcnI methylase yields N4-methylcytosine. FEBS Lett. 1983 Sep 5;161(1):131–134. doi: 10.1016/0014-5793(83)80745-5. [DOI] [PubMed] [Google Scholar]
  16. Jaurin B. A promoter probe vector (pJAC4) that utilizes the ampC beta-lactamase gene of Escherichia coli. Nucleic Acids Res. 1987 Oct 26;15(20):8567–8567. doi: 10.1093/nar/15.20.8567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jiang X. M., Neal B., Santiago F., Lee S. J., Romana L. K., Reeves P. R. Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2). Mol Microbiol. 1991 Mar;5(3):695–713. doi: 10.1111/j.1365-2958.1991.tb00741.x. [DOI] [PubMed] [Google Scholar]
  18. Kessler C., Manta V. Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3). Gene. 1990 Aug 16;92(1-2):1–248. doi: 10.1016/0378-1119(90)90486-b. [DOI] [PubMed] [Google Scholar]
  19. Klena J. D., Pradel E., Schnaitman C. A. Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium. J Bacteriol. 1992 Jul;174(14):4746–4752. doi: 10.1128/jb.174.14.4746-4752.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Miller V. L., Mekalanos J. J. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol. 1988 Jun;170(6):2575–2583. doi: 10.1128/jb.170.6.2575-2583.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Miyahara M., Maruyama T., Wake A., Mise K. Widespread occurrence of the restriction endonuclease YenI, an isoschizomer of PstI, in Yersinia enterocolitica serotype O8. Appl Environ Microbiol. 1988 Feb;54(2):577–580. doi: 10.1128/aem.54.2.577-580.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. O'Farrell P. H., Kutter E., Nakanishi M. A restriction map of the bacteriophage T4 genome. Mol Gen Genet. 1980;179(2):421–435. doi: 10.1007/BF00425473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pradel E., Parker C. T., Schnaitman C. A. Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core. J Bacteriol. 1992 Jul;174(14):4736–4745. doi: 10.1128/jb.174.14.4736-4745.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Price C., Bickle T. A. A possible role for DNA restriction in bacterial evolution. Microbiol Sci. 1986 Oct;3(10):296–299. [PubMed] [Google Scholar]
  26. Razin A., Urieli S., Pollack Y., Gruenbaum Y., Glaser G. Studies on the biological role of dna methylation; IV. Mode of methylation of DNA in E. coli cells. Nucleic Acids Res. 1980 Apr 25;8(8):1783–1792. doi: 10.1093/nar/8.8.1783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schnaitman C. A., Parker C. T., Klena J. D., Pradel E. L., Pearson N. B., Sanderson K. E., MacClachlan P. R. Physical maps of the rfa loci of Escherichia coli K-12 and Salmonella typhimurium. J Bacteriol. 1991 Dec;173(23):7410–7411. doi: 10.1128/jb.173.23.7410-7411.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Scholz P., Haring V., Scherzinger E., Lurz R., Bagdasarian M. M., Schuster H., Bagdasarian M. Replication determinants of the broad host-range plasmid RSF1010. Basic Life Sci. 1985;30:243–259. doi: 10.1007/978-1-4613-2447-8_20. [DOI] [PubMed] [Google Scholar]
  29. Tao T., Bourne J. C., Blumenthal R. M. A family of regulatory genes associated with type II restriction-modification systems. J Bacteriol. 1991 Feb;173(4):1367–1375. doi: 10.1128/jb.173.4.1367-1375.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wells R. D., Neuendorf S. K. Cleavage of "single-stranded" viral DNAs by certain restriction endonucleases. Gene Amplif Anal. 1981;1:101–111. [PubMed] [Google Scholar]
  31. Zhang L., al-Hendy A., Toivanen P., Skurnik M. Genetic organization and sequence of the rfb gene cluster of Yersinia enterocolitica serotype O:3: similarities to the dTDP-L-rhamnose biosynthesis pathway of Salmonella and to the bacterial polysaccharide transport systems. Mol Microbiol. 1993 Jul;9(2):309–321. doi: 10.1111/j.1365-2958.1993.tb01692.x. [DOI] [PubMed] [Google Scholar]
  32. al-Hendy A., Toivanen P., Skurnik M. Expression cloning of Yersinia enterocolitica O:3 rfb gene cluster in Escherichia coli K12. Microb Pathog. 1991 Jan;10(1):47–59. doi: 10.1016/0882-4010(91)90065-i. [DOI] [PubMed] [Google Scholar]
  33. al-Hendy A., Toivanen P., Skurnik M. Rapid method for isolation and staining of bacterial lipopolysaccharide. Microbiol Immunol. 1991;35(4):331–333. doi: 10.1111/j.1348-0421.1991.tb01562.x. [DOI] [PubMed] [Google Scholar]
  34. al-Hendy A., Toivanen P., Skurnik M. The effect of growth temperature on the biosynthesis of Yersinia enterocolitica O:3 lipopolysaccharide: temperature regulates the transcription of the rfb but not of the rfa region. Microb Pathog. 1991 Jan;10(1):81–86. doi: 10.1016/0882-4010(91)90068-l. [DOI] [PubMed] [Google Scholar]
  35. de Lorenzo V., Herrero M., Jakubzik U., Timmis K. N. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol. 1990 Nov;172(11):6568–6572. doi: 10.1128/jb.172.11.6568-6572.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES