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. 1994 Mar;176(6):1782–1786. doi: 10.1128/jb.176.6.1782-1786.1994

Partition of nonreplicating DNA by the par system of bacteriophage P1.

N Treptow 1, R Rosenfeld 1, M Yarmolinsky 1
PMCID: PMC205270  PMID: 8132477

Abstract

P1 plasmid encodes a cis-acting centromere analog, parS, and two Par proteins that together stabilize plasmids by partitioning them to daughter bacteria. We infected immune bacteria with bacteriophage lambda into which parS had been inserted. The presence of P1 Par proteins in the infected cells was found to delay the appearance of cells cured of the nonreplicating, extrachromosomal lambda-parS DNA. This stabilization of lambda-parS, approximated in a computer simulation, demonstrates that active partition by the P1 par system does not require the act of plasmid replication and can be studied in its absence.

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Selected References

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  1. Abeles A. L., Friedman S. A., Austin S. J. Partition of unit-copy miniplasmids to daughter cells. III. The DNA sequence and functional organization of the P1 partition region. J Mol Biol. 1985 Sep 20;185(2):261–272. doi: 10.1016/0022-2836(85)90402-4. [DOI] [PubMed] [Google Scholar]
  2. Austin S. J. Bacterial plasmids that carry two functional centromere analogs are stable and are partitioned faithfully. J Bacteriol. 1984 May;158(2):742–745. doi: 10.1128/jb.158.2.742-745.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Austin S., Abeles A. Partition of unit-copy miniplasmids to daughter cells. I. P1 and F miniplasmids contain discrete, interchangeable sequences sufficient to promote equipartition. J Mol Biol. 1983 Sep 15;169(2):353–372. doi: 10.1016/s0022-2836(83)80055-2. [DOI] [PubMed] [Google Scholar]
  4. Austin S., Abeles A. Partition of unit-copy miniplasmids to daughter cells. II. The partition region of miniplasmid P1 encodes an essential protein and a centromere-like site at which it acts. J Mol Biol. 1983 Sep 15;169(2):373–387. doi: 10.1016/s0022-2836(83)80056-4. [DOI] [PubMed] [Google Scholar]
  5. Austin S., Friedman S., Ludtke D. Partition functions of unit-copy plasmids can stabilize the maintenance of plasmid pBR322 at low copy number. J Bacteriol. 1986 Nov;168(2):1010–1013. doi: 10.1128/jb.168.2.1010-1013.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Austin S., Nordström K. Partition-mediated incompatibility of bacterial plasmids. Cell. 1990 Feb 9;60(3):351–354. doi: 10.1016/0092-8674(90)90584-2. [DOI] [PubMed] [Google Scholar]
  7. Boe L., Gerdes K., Molin S. Effects of genes exerting growth inhibition and plasmid stability on plasmid maintenance. J Bacteriol. 1987 Oct;169(10):4646–4650. doi: 10.1128/jb.169.10.4646-4650.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cerin H., Hackett J. The parVP region of the Salmonella typhimurium virulence plasmid pSLT contains four loci required for incompatibility and partition. Plasmid. 1993 Jul;30(1):30–38. doi: 10.1006/plas.1993.1031. [DOI] [PubMed] [Google Scholar]
  9. Cuzin F., Jacob F. Existence chez Escherichia coli K12 d'une unité génétique de transmission formée de différents réplicons. Ann Inst Pasteur (Paris) 1967 May;112(5):529–545. [PubMed] [Google Scholar]
  10. Della Latta P., Bouanchaud D., Novick R. P. Partition kinetics and thermosensitive replication of pT169, a naturally occurring multicopy tetracycline resistance plasmid of Staphylococcus aureus. Plasmid. 1978 Jun;1(3):366–375. doi: 10.1016/0147-619x(78)90052-5. [DOI] [PubMed] [Google Scholar]
  11. Dennert G., Henning U. Tyrosine-incorporating amber suppressors in Escherichia coli K12. J Mol Biol. 1968 Apr 14;33(1):327–329. doi: 10.1016/0022-2836(68)90300-8. [DOI] [PubMed] [Google Scholar]
  12. Enquist L. W., Weisberg R. A. The red plaque test: a rapid method for identification of excision defective variants of bacteriophage lambda. Virology. 1976 Jul 1;72(1):147–153. doi: 10.1016/0042-6822(76)90319-6. [DOI] [PubMed] [Google Scholar]
  13. Funnell B. E. Participation of Escherichia coli integration host factor in the P1 plasmid partition system. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6657–6661. doi: 10.1073/pnas.85.18.6657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gallie D. R., Kado C. I. Agrobacterium tumefaciens pTAR parA promoter region involved in autoregulation, incompatibility and plasmid partitioning. J Mol Biol. 1987 Feb 5;193(3):465–478. doi: 10.1016/0022-2836(87)90260-9. [DOI] [PubMed] [Google Scholar]
  15. Gottesman M. E., Adhya S., Das A. Transcription antitermination by bacteriophage lambda N gene product. J Mol Biol. 1980 Jun 15;140(1):57–75. doi: 10.1016/0022-2836(80)90356-3. [DOI] [PubMed] [Google Scholar]
  16. Hashimoto-Gotoh T., Ishii K. Temperature sensitive replication plasmids are passively distributed during cell division at non-permissive temperature: a new model for replicon duplication and partitioning. Mol Gen Genet. 1982;187(3):523–525. doi: 10.1007/BF00332639. [DOI] [PubMed] [Google Scholar]
  17. Hiraga S., Ogura T., Niki H., Ichinose C., Mori H. Positioning of replicated chromosomes in Escherichia coli. J Bacteriol. 1990 Jan;172(1):31–39. doi: 10.1128/jb.172.1.31-39.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ike Y., Hashimoto H., Mitsuhashi S. Regular segregation of composite plasmid Rms201. J Bacteriol. 1981 Nov;148(2):534–540. doi: 10.1128/jb.148.2.534-540.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ingmer H., Cohen S. N. The pSC101 par locus alters protein-DNA interactions in vivo at the plasmid replication origin. J Bacteriol. 1993 Sep;175(18):6046–6048. doi: 10.1128/jb.175.18.6046-6048.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lehnherr H., Maguin E., Jafri S., Yarmolinsky M. B. Plasmid addiction genes of bacteriophage P1: doc, which causes cell death on curing of prophage, and phd, which prevents host death when prophage is retained. J Mol Biol. 1993 Oct 5;233(3):414–428. doi: 10.1006/jmbi.1993.1521. [DOI] [PubMed] [Google Scholar]
  21. Ludtke D. N., Eichorn B. G., Austin S. J. Plasmid-partition functions of the P7 prophage. J Mol Biol. 1989 Oct 5;209(3):393–406. doi: 10.1016/0022-2836(89)90005-3. [DOI] [PubMed] [Google Scholar]
  22. Manen D., Goebel T., Caro L. The par region of pSC101 affects plasmid copy number as well as stability. Mol Microbiol. 1990 Nov;4(11):1839–1846. doi: 10.1111/j.1365-2958.1990.tb02032.x. [DOI] [PubMed] [Google Scholar]
  23. Miller C. A., Cohen S. N. The partition (par) locus of pSC101 is an enhancer of plasmid incompatibility. Mol Microbiol. 1993 Aug;9(4):695–702. doi: 10.1111/j.1365-2958.1993.tb01730.x. [DOI] [PubMed] [Google Scholar]
  24. Mizusawa S., Ward D. F. A bacteriophage lambda vector for cloning with BamHI and Sau3A. Gene. 1982 Dec;20(3):317–322. doi: 10.1016/0378-1119(82)90200-1. [DOI] [PubMed] [Google Scholar]
  25. Nordström K. Equipartition and other modes of partition: on the interpretation of curing kinetics using rep(ts) plasmids. Mol Gen Genet. 1984;198(2):185–186. doi: 10.1007/BF00328723. [DOI] [PubMed] [Google Scholar]
  26. Novick R. P. Studies on plasmid replication. III. Isolation and characterization of replication-defective mutants. Mol Gen Genet. 1974;135(2):131–147. doi: 10.1007/BF00264781. [DOI] [PubMed] [Google Scholar]
  27. Ogura T., Hiraga S. Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition. Cell. 1983 Feb;32(2):351–360. doi: 10.1016/0092-8674(83)90454-3. [DOI] [PubMed] [Google Scholar]
  28. Prentki P., Chandler M., Caro L. Replication of prophage P1 during the cell cycle of Escherichia coli. Mol Gen Genet. 1977 Mar 28;152(1):71–76. doi: 10.1007/BF00264942. [DOI] [PubMed] [Google Scholar]
  29. Rosner J. L. Formation, induction, and curing of bacteriophage P1 lysogens. Virology. 1972 Jun;48(3):679–689. doi: 10.1016/0042-6822(72)90152-3. [DOI] [PubMed] [Google Scholar]
  30. Rothfield L. I., Cook W. R. Periseptal annuli: organelles involved in the bacterial cell division process. Microbiol Sci. 1988 Jun;5(6):182–185. [PubMed] [Google Scholar]
  31. Tinge S. A., Curtiss R., 3rd Isolation of the replication and partitioning regions of the Salmonella typhimurium virulence plasmid and stabilization of heterologous replicons. J Bacteriol. 1990 Sep;172(9):5266–5277. doi: 10.1128/jb.172.9.5266-5277.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Williams D. R., Thomas C. M. Active partitioning of bacterial plasmids. J Gen Microbiol. 1992 Jan;138(1):1–16. doi: 10.1099/00221287-138-1-1. [DOI] [PubMed] [Google Scholar]
  33. Zagursky R. J., Berman M. L. Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing. Gene. 1984 Feb;27(2):183–191. doi: 10.1016/0378-1119(84)90139-2. [DOI] [PubMed] [Google Scholar]

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