Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Apr;176(8):2435–2438. doi: 10.1128/jb.176.8.2435-2438.1994

Regulation of sigma D expression and activity by spo0, abrB, and sin gene products in Bacillus subtilis.

L M Márquez-Magaña 1, D B Mirel 1, M J Chamberlin 1
PMCID: PMC205369  PMID: 8157613

Abstract

Expression of sigma D protein and of the hag gene, which is transcribed by the sigma D holoenzyme, is not dependent on spo0, abrB, or sin gene products in Bacillus subtilis. Preliminary results, however, suggest that a signal mediated by the spo0K locus may be responsible for the inhibition of sigma D activity during the stationary phase.

Full text

PDF
2435

Images in this article

2436Image
on p.2436
2436Image
on p.2436
2437Image
on p.2437
2437Image
on p.2437

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Errington J., Appleby L., Daniel R. A., Goodfellow H., Partridge S. R., Yudkin M. D. Structure and function of the spoIIIJ gene of Bacillus subtilis: a vegetatively expressed gene that is essential for sigma G activity at an intermediate stage of sporulation. J Gen Microbiol. 1992 Dec;138(12):2609–2618. doi: 10.1099/00221287-138-12-2609. [DOI] [PubMed] [Google Scholar]
  3. Gaur N. K., Dubnau E., Smith I. Characterization of a cloned Bacillus subtilis gene that inhibits sporulation in multiple copies. J Bacteriol. 1986 Nov;168(2):860–869. doi: 10.1128/jb.168.2.860-869.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gaur N. K., Oppenheim J., Smith I. The Bacillus subtilis sin gene, a regulator of alternate developmental processes, codes for a DNA-binding protein. J Bacteriol. 1991 Jan;173(2):678–686. doi: 10.1128/jb.173.2.678-686.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gilman M. Z., Chamberlin M. J. Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase. Cell. 1983 Nov;35(1):285–293. doi: 10.1016/0092-8674(83)90231-3. [DOI] [PubMed] [Google Scholar]
  6. Grossman A. D. Integration of developmental signals and the initiation of sporulation in B. subtilis. Cell. 1991 Apr 5;65(1):5–8. doi: 10.1016/0092-8674(91)90353-z. [DOI] [PubMed] [Google Scholar]
  7. Grossman A. D., Lewis T., Levin N., DeVivo R. Suppressors of a spo0A missense mutation and their effects on sporulation in Bacillus subtilis. Biochimie. 1992 Jul-Aug;74(7-8):679–688. doi: 10.1016/0300-9084(92)90140-a. [DOI] [PubMed] [Google Scholar]
  8. Guillen N., Weinrauch Y., Dubnau D. A. Cloning and characterization of the regulatory Bacillus subtilis competence genes comA and comB. J Bacteriol. 1989 Oct;171(10):5354–5361. doi: 10.1128/jb.171.10.5354-5361.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Helmann J. D., Márquez L. M., Chamberlin M. J. Cloning, sequencing, and disruption of the Bacillus subtilis sigma 28 gene. J Bacteriol. 1988 Apr;170(4):1568–1574. doi: 10.1128/jb.170.4.1568-1574.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Henner D. J., Yang M., Ferrari E. Localization of Bacillus subtilis sacU(Hy) mutations to two linked genes with similarities to the conserved procaryotic family of two-component signalling systems. J Bacteriol. 1988 Nov;170(11):5102–5109. doi: 10.1128/jb.170.11.5102-5109.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hoch J. A. Genetics of bacterial sporulation. Adv Genet. 1976;18:69–98. doi: 10.1016/s0065-2660(08)60437-x. [DOI] [PubMed] [Google Scholar]
  12. Lazarevic V., Margot P., Soldo B., Karamata D. Sequencing and analysis of the Bacillus subtilis lytRABC divergon: a regulatory unit encompassing the structural genes of the N-acetylmuramoyl-L-alanine amidase and its modifier. J Gen Microbiol. 1992 Sep;138(9):1949–1961. doi: 10.1099/00221287-138-9-1949. [DOI] [PubMed] [Google Scholar]
  13. Lepesant J. A., Kunst F., Lepesant-Kejzlarová J., Dedonder R. Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg. Mol Gen Genet. 1972;118(2):135–160. doi: 10.1007/BF00267084. [DOI] [PubMed] [Google Scholar]
  14. Losick R., Youngman P., Piggot P. J. Genetics of endospore formation in Bacillus subtilis. Annu Rev Genet. 1986;20:625–669. doi: 10.1146/annurev.ge.20.120186.003205. [DOI] [PubMed] [Google Scholar]
  15. Massie H. R., Zimm B. H. Molecular weight of the DNA in the chromosomes of E. coli and B. subtilis. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1636–1641. doi: 10.1073/pnas.54.6.1636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mirel D. B., Chamberlin M. J. The Bacillus subtilis flagellin gene (hag) is transcribed by the sigma 28 form of RNA polymerase. J Bacteriol. 1989 Jun;171(6):3095–3101. doi: 10.1128/jb.171.6.3095-3101.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mirel D. B., Lustre V. M., Chamberlin M. J. An operon of Bacillus subtilis motility genes transcribed by the sigma D form of RNA polymerase. J Bacteriol. 1992 Jul;174(13):4197–4204. doi: 10.1128/jb.174.13.4197-4204.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Msadek T., Kunst F., Henner D., Klier A., Rapoport G., Dedonder R. Signal transduction pathway controlling synthesis of a class of degradative enzymes in Bacillus subtilis: expression of the regulatory genes and analysis of mutations in degS and degU. J Bacteriol. 1990 Feb;172(2):824–834. doi: 10.1128/jb.172.2.824-834.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Márquez-Magaña L. M., Chamberlin M. J. Characterization of the sigD transcription unit of Bacillus subtilis. J Bacteriol. 1994 Apr;176(8):2427–2434. doi: 10.1128/jb.176.8.2427-2434.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Márquez L. M., Helmann J. D., Ferrari E., Parker H. M., Ordal G. W., Chamberlin M. J. Studies of sigma D-dependent functions in Bacillus subtilis. J Bacteriol. 1990 Jun;172(6):3435–3443. doi: 10.1128/jb.172.6.3435-3443.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Perego M., Higgins C. F., Pearce S. R., Gallagher M. P., Hoch J. A. The oligopeptide transport system of Bacillus subtilis plays a role in the initiation of sporulation. Mol Microbiol. 1991 Jan;5(1):173–185. doi: 10.1111/j.1365-2958.1991.tb01838.x. [DOI] [PubMed] [Google Scholar]
  22. Piggot P. J., Coote J. G. Genetic aspects of bacterial endospore formation. Bacteriol Rev. 1976 Dec;40(4):908–962. doi: 10.1128/br.40.4.908-962.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pooley H. M., Karamata D. Genetic analysis of autolysin-deficient and flagellaless mutants of Bacillus subtilis. J Bacteriol. 1984 Dec;160(3):1123–1129. doi: 10.1128/jb.160.3.1123-1129.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rudner D. Z., LeDeaux J. R., Ireton K., Grossman A. D. The spo0K locus of Bacillus subtilis is homologous to the oligopeptide permease locus and is required for sporulation and competence. J Bacteriol. 1991 Feb;173(4):1388–1398. doi: 10.1128/jb.173.4.1388-1398.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sekiguchi J., Ohsu H., Kuroda A., Moriyama H., Akamatsu T. Nucleotide sequences of the Bacillus subtilis flaD locus and a B. licheniformis homologue affecting the autolysin level and flagellation. J Gen Microbiol. 1990 Jul;136(7):1223–1230. doi: 10.1099/00221287-136-7-1223. [DOI] [PubMed] [Google Scholar]
  26. Toma S., Del Bue M., Pirola A., Grandi G. nprR1 and nprR2 regulatory regions for neutral protease expression in Bacillus subtilis. J Bacteriol. 1986 Aug;167(2):740–743. doi: 10.1128/jb.167.2.740-743.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Trowsdale J., Chen S. M., Hoch J. A. Genetic analysis of a class of polymyxin resistant partial revertants of stage O sporulation mutants of Bacillus subtilis: map of the chromosome region near the origin of replication. Mol Gen Genet. 1979 May 23;173(1):61–70. doi: 10.1007/BF00267691. [DOI] [PubMed] [Google Scholar]
  28. Wang P. Z., Doi R. H. Overlapping promoters transcribed by bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases. J Biol Chem. 1984 Jul 10;259(13):8619–8625. [PubMed] [Google Scholar]
  29. Wiggs J. L., Gilman M. Z., Chamberlin M. J. Heterogeneity of RNA polymerase in Bacillus subtilis: evidence for an additional sigma factor in vegetative cells. Proc Natl Acad Sci U S A. 1981 May;78(5):2762–2766. doi: 10.1073/pnas.78.5.2762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yang M. Y., Ferrari E., Henner D. J. Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation. J Bacteriol. 1984 Oct;160(1):15–21. doi: 10.1128/jb.160.1.15-21.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zuberi A. R., Ying C. W., Weinreich M. R., Ordal G. W. Transcriptional organization of a cloned chemotaxis locus of Bacillus subtilis. J Bacteriol. 1990 Apr;172(4):1870–1876. doi: 10.1128/jb.172.4.1870-1876.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES