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. 1994 May;176(9):2763–2766. doi: 10.1128/jb.176.9.2763-2766.1994

Use of lac gene fusions in the analysis of Acholeplasma upstream gene regulatory sequences.

K L Knudtson 1, F C Minion 1
PMCID: PMC205422  PMID: 8169231

Abstract

The objective of this study was to develop a means of identifying and analyzing mycoplasma gene regulatory elements. Analysis of Acholeplasma oculi ISM1499 transcriptional sequences was accomplished using a promoterless lacZ reporter gene and an integrative vector strategy. Seven promoter-containing fragments were identified, levels of beta-galactosidase were determined, and transcriptional start sites were mapped.

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Selected References

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