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. 1994 May;176(9):2767–2770. doi: 10.1128/jb.176.9.2767-2770.1994

Overexpression of genes of an extreme thermophile Thermus thermophilus, in Escherichia coli cells.

M Ishida 1, T Oshima 1
PMCID: PMC205423  PMID: 8169232

Abstract

The 3-isopropylmalate dehydrogenase gene from an extreme thermophile, Thermus thermophilus, was not expressed in Escherichia coli unless a palindromic structure around the ribosome binding site was eliminated or a leader open reading frame was introduced into the upstream flanking region of the gene. This report suggests a way to increase the expression of this gene, with a high G+C content, in E. coli.

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Selected References

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