Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 May;176(10):3089–3091. doi: 10.1128/jb.176.10.3089-3091.1994

A single promoter sequence recognized by a newly identified alternate sigma factor directs expression of pathogenicity and host range determinants in Pseudomonas syringae.

Y Xiao 1, S W Hutcheson 1
PMCID: PMC205470  PMID: 8188613

Abstract

A conserved sequence motif associated with transcription of avr genes was identified in the promoter regions of six Pseudomonas syringae pv. syringae Pss61 hrp operons. A 34-bp fragment carrying this motif was cloned from the HrpZ promoter region and was shown to confer HrpL-dependent promoter activity. Expression of pathogenicity and host range determinants in P. syringae strains is thus directed by the apparent alternate sigma factor HrpL.

Full text

PDF
3089

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976 Jul 5;104(3):541–555. doi: 10.1016/0022-2836(76)90119-4. [DOI] [PubMed] [Google Scholar]
  2. Fenselau S., Balbo I., Bonas U. Determinants of pathogenicity in Xanthomonas campestris pv. vesicatoria are related to proteins involved in secretion in bacterial pathogens of animals. Mol Plant Microbe Interact. 1992 Sep-Oct;5(5):390–396. doi: 10.1094/mpmi-5-390. [DOI] [PubMed] [Google Scholar]
  3. Gough C. L., Genin S., Zischek C., Boucher C. A. hrp genes of Pseudomonas solanacearum are homologous to pathogenicity determinants of animal pathogenic bacteria and are conserved among plant pathogenic bacteria. Mol Plant Microbe Interact. 1992 Sep-Oct;5(5):384–389. doi: 10.1094/mpmi-5-384. [DOI] [PubMed] [Google Scholar]
  4. He S. Y., Huang H. C., Collmer A. Pseudomonas syringae pv. syringae harpinPss: a protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plants. Cell. 1993 Jul 2;73(7):1255–1266. doi: 10.1016/0092-8674(93)90354-s. [DOI] [PubMed] [Google Scholar]
  5. Heu S., Hutcheson S. W. Nucleotide sequence and properties of the hrmA locus associated with the Pseudomonas syringae pv. syringae 61 hrp gene cluster. Mol Plant Microbe Interact. 1993 Sep-Oct;6(5):553–564. doi: 10.1094/mpmi-6-553. [DOI] [PubMed] [Google Scholar]
  6. Huang H. C., He S. Y., Bauer D. W., Collmer A. The Pseudomonas syringae pv. syringae 61 hrpH product, an envelope protein required for elicitation of the hypersensitive response in plants. J Bacteriol. 1992 Nov;174(21):6878–6885. doi: 10.1128/jb.174.21.6878-6885.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huang H. C., Schuurink R., Denny T. P., Atkinson M. M., Baker C. J., Yucel I., Hutcheson S. W., Collmer A. Molecular cloning of a Pseudomonas syringae pv. syringae gene cluster that enables Pseudomonas fluorescens to elicit the hypersensitive response in tobacco plants. J Bacteriol. 1988 Oct;170(10):4748–4756. doi: 10.1128/jb.170.10.4748-4756.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huang H. C., Xiao Y., Lin R. H., Lu Y., Hutcheson S. W., Collmer A. Characterization of the Pseudomonas syringae pv. syringae 61 hrpJ and hrpI genes: homology of HrpI to a superfamily of proteins associated with protein translocation. Mol Plant Microbe Interact. 1993 Jul-Aug;6(4):515–520. doi: 10.1094/mpmi-6-515. [DOI] [PubMed] [Google Scholar]
  9. Huynh T. V., Dahlbeck D., Staskawicz B. J. Bacterial blight of soybean: regulation of a pathogen gene determining host cultivar specificity. Science. 1989 Sep 22;245(4924):1374–1377. doi: 10.1126/science.2781284. [DOI] [PubMed] [Google Scholar]
  10. Innes R. W., Bent A. F., Kunkel B. N., Bisgrove S. R., Staskawicz B. J. Molecular analysis of avirulence gene avrRpt2 and identification of a putative regulatory sequence common to all known Pseudomonas syringae avirulence genes. J Bacteriol. 1993 Aug;175(15):4859–4869. doi: 10.1128/jb.175.15.4859-4869.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KING E. O., WARD M. K., RANEY D. E. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed] [Google Scholar]
  12. Lonetto M., Gribskov M., Gross C. A. The sigma 70 family: sequence conservation and evolutionary relationships. J Bacteriol. 1992 Jun;174(12):3843–3849. doi: 10.1128/jb.174.12.3843-3849.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mohr C. D., Martin D. W., Konyecsni W. M., Govan J. R., Lory S., Deretic V. Role of the far-upstream sites of the algD promoter and the algR and rpoN genes in environmental modulation of mucoidy in Pseudomonas aeruginosa. J Bacteriol. 1990 Nov;172(11):6576–6580. doi: 10.1128/jb.172.11.6576-6580.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rahme L. G., Mindrinos M. N., Panopoulos N. J. Genetic and transcriptional organization of the hrp cluster of Pseudomonas syringae pv. phaseolicola. J Bacteriol. 1991 Jan;173(2):575–586. doi: 10.1128/jb.173.2.575-586.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rahme L. G., Mindrinos M. N., Panopoulos N. J. Plant and environmental sensory signals control the expression of hrp genes in Pseudomonas syringae pv. phaseolicola. J Bacteriol. 1992 Jun;174(11):3499–3507. doi: 10.1128/jb.174.11.3499-3507.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salmeron J. M., Staskawicz B. J. Molecular characterization and hrp dependence of the avirulence gene avrPto from Pseudomonas syringae pv. tomato [corrected]. Mol Gen Genet. 1993 May;239(1-2):6–16. doi: 10.1007/BF00281595. [DOI] [PubMed] [Google Scholar]
  17. Shen H., Keen N. T. Characterization of the promoter of avirulence gene D from Pseudomonas syringae pv. tomato. J Bacteriol. 1993 Sep;175(18):5916–5924. doi: 10.1128/jb.175.18.5916-5924.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Van den Eede G., Deblaere R., Goethals K., Van Montagu M., Holsters M. Broad host range and promoter selection vectors for bacteria that interact with plants. Mol Plant Microbe Interact. 1992 May-Jun;5(3):228–234. doi: 10.1094/mpmi-5-228. [DOI] [PubMed] [Google Scholar]
  19. Xiao Y., Heu S., Yi J., Lu Y., Hutcheson S. W. Identification of a putative alternate sigma factor and characterization of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes. J Bacteriol. 1994 Feb;176(4):1025–1036. doi: 10.1128/jb.176.4.1025-1036.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Xiao Y., Lu Y., Heu S., Hutcheson S. W. Organization and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp cluster. J Bacteriol. 1992 Mar;174(6):1734–1741. doi: 10.1128/jb.174.6.1734-1741.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES