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. 1994 Jun;176(11):3360–3367. doi: 10.1128/jb.176.11.3360-3367.1994

Stress induction of clpC in Bacillus subtilis and its involvement in stress tolerance.

E Krüger 1, U Völker 1, M Hecker 1
PMCID: PMC205508  PMID: 8195092

Abstract

A member of the clpC subfamily of stress response-related Clp ATPases was cloned from Bacillus subtilis. The B. subtilis clpC gene was induced in response to various stresses, including heat shock. Its product was identified as a general stress protein (Gsp12) described previously. A dramatic increase in the amount of clpC mRNA immediately after exposure to multiple stresses suggested regulation on a transcriptional level. Induction by heat shock was independent of the alternative sigma factor SigB, indicating a new mechanism of heat shock induction in B. subtilis. A clpC insertional mutant had an impaired tolerance for heat shock and salt stress. Furthermore, the mutation triggered the formation of elongated cells, a phenomenon particularly pronounced during stress.

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Selected References

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