Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Jun;176(11):3386–3388. doi: 10.1128/jb.176.11.3386-3388.1994

Length of the coupling sequence of Tn916.

C K Rudy 1, J R Scott 1
PMCID: PMC205512  PMID: 8195096

Abstract

The coupling sequences of conjugative transposons are short variable sequences derived from the DNA flanking the transposon insertion site. We show here that for Tn916 the left coupling sequence is 6 bases long. The right-hand end of the transposon can excise with either four or five T's, but integration occurs to restore the five T's at the transposon's right end.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Caillaud F., Courvalin P. Nucleotide sequence of the ends of the conjugative shuttle transposon Tn1545. Mol Gen Genet. 1987 Aug;209(1):110–115. doi: 10.1007/BF00329844. [DOI] [PubMed] [Google Scholar]
  3. Caparon M. G., Scott J. R. Excision and insertion of the conjugative transposon Tn916 involves a novel recombination mechanism. Cell. 1989 Dec 22;59(6):1027–1034. doi: 10.1016/0092-8674(89)90759-9. [DOI] [PubMed] [Google Scholar]
  4. Carpenter P. B., Ordal G. W. Bacillus subtilis FlhA: a flagellar protein related to a new family of signal-transducing receptors. Mol Microbiol. 1993 Mar;7(5):735–743. doi: 10.1111/j.1365-2958.1993.tb01164.x. [DOI] [PubMed] [Google Scholar]
  5. Clewell D. B., Flannagan S. E., Ike Y., Jones J. M., Gawron-Burke C. Sequence analysis of termini of conjugative transposon Tn916. J Bacteriol. 1988 Jul;170(7):3046–3052. doi: 10.1128/jb.170.7.3046-3052.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gaillard J. L., Berche P., Frehel C., Gouin E., Cossart P. Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocci. Cell. 1991 Jun 28;65(7):1127–1141. doi: 10.1016/0092-8674(91)90009-n. [DOI] [PubMed] [Google Scholar]
  7. Ike Y., Flannagan S. E., Clewell D. B. Hyperhemolytic phenomena associated with insertions of Tn916 into the hemolysin determinant of Enterococcus faecalis plasmid pAD1. J Bacteriol. 1992 Mar;174(6):1801–1809. doi: 10.1128/jb.174.6.1801-1809.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Poyart-Salmeron C., Trieu-Cuot P., Carlier C., Courvalin P. Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: homologies with other site-specific recombinases. EMBO J. 1989 Aug;8(8):2425–2433. doi: 10.1002/j.1460-2075.1989.tb08373.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Poyart-Salmeron C., Trieu-Cuot P., Carlier C., Courvalin P. The integration-excision system of the conjugative transposon Tn 1545 is structurally and functionally related to those of lambdoid phages. Mol Microbiol. 1990 Sep;4(9):1513–1521. doi: 10.1111/j.1365-2958.1990.tb02062.x. [DOI] [PubMed] [Google Scholar]
  10. Sasaki Y., Taketomo N., Sasaki T. Factors affecting transfer frequency of pAM beta 1 from Streptococcus faecalis to Lactobacillus plantarum. J Bacteriol. 1988 Dec;170(12):5939–5942. doi: 10.1128/jb.170.12.5939-5942.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Scott J. R., Bringel F., Marra D., Van Alstine G., Rudy C. K. Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. Mol Microbiol. 1994 Mar;11(6):1099–1108. doi: 10.1111/j.1365-2958.1994.tb00386.x. [DOI] [PubMed] [Google Scholar]
  12. Scott J. R., Kirchman P. A., Caparon M. G. An intermediate in transposition of the conjugative transposon Tn916. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4809–4813. doi: 10.1073/pnas.85.13.4809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scott J. R. Sex and the single circle: conjugative transposition. J Bacteriol. 1992 Oct;174(19):6005–6010. doi: 10.1128/jb.174.19.6005-6010.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Trieu-Cuot P., Poyart-Salmeron C., Carlier C., Courvalin P. Sequence requirements for target activity in site-specific recombination mediated by the Int protein of transposon Tn 1545. Mol Microbiol. 1993 Apr;8(1):179–185. doi: 10.1111/j.1365-2958.1993.tb01214.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES