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. 1994 Jun;176(12):3820–3823. doi: 10.1128/jb.176.12.3820-3823.1994

Expression of a yeast intron-containing tRNA in the archaeon Haloferax volcanii.

J R Palmer 1, D T Nieuwlandt 1, C J Daniels 1
PMCID: PMC205573  PMID: 8206862

Abstract

Expression of the yeast tRNAPro(UGG) gene in Haloferax volcanii resulted in the production of a single stable transcript that had not undergone intron processing or processing of 5' and 3' flanking sequences. Mutation of the exon-intron boundary region of this RNA to produce a precursor RNA with the preferred halobacterial consensus exon-intron boundary structure did not restore intron processing. Processing of 5' and 3' flanking sequences was restored when the acceptor stem U6-U67 pair was changed to A6-U67. The significance of these results in defining the recognition requirements of tRNA maturation enzymes in the halophilic domain Archaea is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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