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. 1994 Jul;176(13):3851–3858. doi: 10.1128/jb.176.13.3851-3858.1994

Diversity of tandemly repetitive sequences due to short periodic repetitions in the chromosomes of Candida albicans.

H Chibana 1, S Iwaguchi 1, M Homma 1, A Chindamporn 1, Y Nakagawa 1, K Tanaka 1
PMCID: PMC205581  PMID: 8021166

Abstract

In a previous study, a repeated sequence, RPS1, was cloned from the genomic DNA of Candida albicans. It was 2.1 kb in length and was tandemly repeated in a limited region of almost all of the chromosomes. In this study, we examined and characterized the diversity of the repeating structure of the RPS units were of 2.1, 2.3, 2.5, and 2.9 kbp in length after digestion of the genomic DNA with SmaI and 2.1 and 2.3 kbp after digestion with PstI, with the differences being multiples of approximately 0.2 kbp. Moreover, one or two types of RPS unit were present specifically on each chromosome. We cloned 14 RPS units from the mixed DNA of chromosomes 1 and 2 and 59 RPS units from chromosome 6. These RPS units were classified into four types by their SfiI digestion profiles and chromosomal origins. Sequence comparisons revealed a tandem arrangement of internal, small repeating units of 172 bp. This unit of repetition was designated alt (C. albicans tandem repeating unit). The size of RPS units was variable, with sizes representing a series of increments of approximately 0.2 kbp that corresponded to the alt sequence. By contrast, the sequences other than the tandem repeats of alts were highly conserved, with homology of more than 98% among all cloned RPS units. These results suggested that RPS plays an important role in the organization and function of the chromosomes of C. albicans even though the actual function of RPS has not yet been clarified. Structural features of RPS that contains the repeated alt sequence are discussed in relation to human alpha-satellite DNA with its tandem repeats of about 170 bp that are similar in size to alt, the repetition of which is responsible for the variations in the size of the higher-order repeats.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asakura K., Iwaguchi S., Homma M., Sukai T., Higashide K., Tanaka K. Electrophoretic karyotypes of clinically isolated yeasts of Candida albicans and C. glabrata. J Gen Microbiol. 1991 Nov;137(11):2531–2538. doi: 10.1099/00221287-137-11-2531. [DOI] [PubMed] [Google Scholar]
  2. Chindamporn A., Iwaguchi S., Nakagawa Y., Homma M., Tanaka K. Clonal size-variation of rDNA cluster region on chromosome XII of Saccharomyces cerevisiae. J Gen Microbiol. 1993 Jul;139(7):1409–1415. doi: 10.1099/00221287-139-7-1409. [DOI] [PubMed] [Google Scholar]
  3. Chu W. S., Magee B. B., Magee P. T. Construction of an SfiI macrorestriction map of the Candida albicans genome. J Bacteriol. 1993 Oct;175(20):6637–6651. doi: 10.1128/jb.175.20.6637-6651.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cutler J. E., Glee P. M., Horn H. L. Candida albicans- and Candida stellatoidea-specific DNA fragment. J Clin Microbiol. 1988 Sep;26(9):1720–1724. doi: 10.1128/jcm.26.9.1720-1724.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doi M., Homma M., Chindamporn A., Tanaka K. Estimation of chromosome number and size by pulsed-field gel electrophoresis (PFGE) in medically important Candida species. J Gen Microbiol. 1992 Oct;138(10):2243–2251. doi: 10.1099/00221287-138-10-2243. [DOI] [PubMed] [Google Scholar]
  6. Fan J. B., Chikashige Y., Smith C. L., Niwa O., Yanagida M., Cantor C. R. Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome. Nucleic Acids Res. 1989 Apr 11;17(7):2801–2818. doi: 10.1093/nar/17.7.2801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hawley R. S., Marcus C. H. Recombinational controls of rDNA redundancy in Drosophila. Annu Rev Genet. 1989;23:87–120. doi: 10.1146/annurev.ge.23.120189.000511. [DOI] [PubMed] [Google Scholar]
  8. Iwaguchi S., Homma M., Chibana H., Tanaka K. Isolation and characterization of a repeated sequence (RPS1) of Candida albicans. J Gen Microbiol. 1992 Sep;138(9):1893–1900. doi: 10.1099/00221287-138-9-1893. [DOI] [PubMed] [Google Scholar]
  9. Iwaguchi S., Homma M., Tanaka K. Clonal variation of chromosome size derived from the rDNA cluster region in Candida albicans. J Gen Microbiol. 1992 Jun;138(6):1177–1184. doi: 10.1099/00221287-138-6-1177. [DOI] [PubMed] [Google Scholar]
  10. Iwaguchi S., Homma M., Tanaka K. Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans. J Gen Microbiol. 1990 Dec;136(12):2433–2442. doi: 10.1099/00221287-136-12-2433. [DOI] [PubMed] [Google Scholar]
  11. Jeffreys A. J., Wilson V., Thein S. L. Hypervariable 'minisatellite' regions in human DNA. Nature. 1985 Mar 7;314(6006):67–73. doi: 10.1038/314067a0. [DOI] [PubMed] [Google Scholar]
  12. Keil R. L., Roeder G. S. Cis-acting, recombination-stimulating activity in a fragment of the ribosomal DNA of S. cerevisiae. Cell. 1984 Dec;39(2 Pt 1):377–386. doi: 10.1016/0092-8674(84)90016-3. [DOI] [PubMed] [Google Scholar]
  13. Lasker B. A., Carle G. F., Kobayashi G. S., Medoff G. Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques. Nucleic Acids Res. 1989 May 25;17(10):3783–3793. doi: 10.1093/nar/17.10.3783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lasker B. A., Page L. S., Lott T. J., Kobayashi G. S. Isolation, characterization, and sequencing of Candida albicans repetitive element 2. Gene. 1992 Jul 1;116(1):51–57. doi: 10.1016/0378-1119(92)90628-3. [DOI] [PubMed] [Google Scholar]
  15. Lott T. J., Boiron P., Reiss E. An electrophoretic karyotype for Candida albicans reveals large chromosomes in multiples. Mol Gen Genet. 1987 Aug;209(1):170–174. doi: 10.1007/BF00329854. [DOI] [PubMed] [Google Scholar]
  16. Magee B. B., Magee P. T. Electrophoretic karyotypes and chromosome numbers in Candida species. J Gen Microbiol. 1987 Feb;133(2):425–430. doi: 10.1099/00221287-133-2-425. [DOI] [PubMed] [Google Scholar]
  17. Merz W. G., Connelly C., Hieter P. Variation of electrophoretic karyotypes among clinical isolates of Candida albicans. J Clin Microbiol. 1988 May;26(5):842–845. doi: 10.1128/jcm.26.5.842-845.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miyakawa Y., Mabuchi T., Kagaya K., Fukazawa Y. Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction. J Clin Microbiol. 1992 Apr;30(4):894–900. doi: 10.1128/jcm.30.4.894-900.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mortimer R. K., Schild D. Genetic map of Saccharomyces cerevisiae, edition 9. Microbiol Rev. 1985 Sep;49(3):181–213. doi: 10.1128/mr.49.3.181-213.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Petes T. D., Hill C. W. Recombination between repeated genes in microorganisms. Annu Rev Genet. 1988;22:147–168. doi: 10.1146/annurev.ge.22.120188.001051. [DOI] [PubMed] [Google Scholar]
  21. Rustchenko-Bulgac E. P. Variations of Candida albicans electrophoretic karyotypes. J Bacteriol. 1991 Oct;173(20):6586–6596. doi: 10.1128/jb.173.20.6586-6596.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sadhu C., McEachern M. J., Rustchenko-Bulgac E. P., Schmid J., Soll D. R., Hicks J. B. Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification. J Bacteriol. 1991 Jan;173(2):842–850. doi: 10.1128/jb.173.2.842-850.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scherer S., Stevens D. A. A Candida albicans dispersed, repeated gene family and its epidemiologic applications. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1452–1456. doi: 10.1073/pnas.85.5.1452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Snell R. G., Hermans I. F., Wilkins R. J., Corner B. E. Chromosomal variations in Candida albicans. Nucleic Acids Res. 1987 Apr 24;15(8):3625–3625. doi: 10.1093/nar/15.8.3625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Szostak J. W., Wu R. Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. Nature. 1980 Apr 3;284(5755):426–430. doi: 10.1038/284426a0. [DOI] [PubMed] [Google Scholar]
  26. Thrash-Bingham C., Gorman J. A. DNA translocations contribute to chromosome length polymorphisms in Candida albicans. Curr Genet. 1992 Aug;22(2):93–100. doi: 10.1007/BF00351467. [DOI] [PubMed] [Google Scholar]
  27. Thrash-Bingham C., Gorman J. A. Identification, characterization and sequence of Candida albicans repetitive DNAs Rel-1 and Rel-2. Curr Genet. 1993 May-Jun;23(5-6):455–462. doi: 10.1007/BF00312634. [DOI] [PubMed] [Google Scholar]
  28. Van Arsdell S. W., Weiner A. M. Human genes for U2 small nuclear RNA are tandemly repeated. Mol Cell Biol. 1984 Mar;4(3):492–499. doi: 10.1128/mcb.4.3.492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Weiner A. M., Denison R. A. Either gene amplification or gene conversion may maintain the homogeneity of the multigene family encoding human U1 small nuclear RNA. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):1141–1149. doi: 10.1101/sqb.1983.047.01.129. [DOI] [PubMed] [Google Scholar]
  30. Wickes B., Staudinger J., Magee B. B., Kwon-Chung K. J., Magee P. T., Scherer S. Physical and genetic mapping of Candida albicans: several genes previously assigned to chromosome 1 map to chromosome R, the rDNA-containing linkage group. Infect Immun. 1991 Jul;59(7):2480–2484. doi: 10.1128/iai.59.7.2480-2484.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Willard H. F. Centromeres of mammalian chromosomes. Trends Genet. 1990 Dec;6(12):410–416. doi: 10.1016/0168-9525(90)90302-m. [DOI] [PubMed] [Google Scholar]

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