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. 1994 Jul;176(13):4173–4176. doi: 10.1128/jb.176.13.4173-4176.1994

Transformation of Actinomyces spp. by a gram-negative broad-host-range plasmid.

M K Yeung 1, C S Kozelsky 1
PMCID: PMC205619  PMID: 8021199

Abstract

The gram-negative broad-host-range vector pJRD215 was transferred by electroporation into strains of Actinomyces viscosus or Actinomyces naeslundii at efficiencies which ranged from 10(2) to 10(7) transformants per microgram of plasmid DNA. The Actinomyces transformants expressed pJRD215-encoded resistance to kanamycin and streptomycin. Moreover, the transforming plasmid DNA had not undergone any deletions or rearrangements, nor had it integrated into the genomes of these strains.

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Selected References

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