Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Jul;176(13):4182–4186. doi: 10.1128/jb.176.13.4182-4186.1994

Molecular analysis of the F plasmid traVR region: traV encodes a lipoprotein.

T J Doran 1, S M Loh 1, N Firth 1, R A Skurray 1
PMCID: PMC205621  PMID: 8021201

Abstract

The nucleotide sequences of the conjugative F plasmid transfer region genes, traV and traR, have been determined. The deduced amino acid sequence of TraV indicated that it may be a lipoprotein; this was confirmed by examining the effect of globomycin on traV-encoded polypeptides synthesized in minicells. An open reading frame that may represent a previously undetected transfer gene, now designated trbG, was identified immediately upstream of traV. The deduced product of traR was found to share amino acid similarity with proteins from the bacteriophages 186 and P2 and with the dosage-dependent dnaK suppressor DksA.

Full text

PDF
4182

Images in this article

4183Image
on p.4183

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Skurray R. A., Thompson R., Helmuth R., Hall S., Beutin L., Clark A. J. Assignment of tra cistrons to EcoRI fragments of F sex factor DNA. J Bacteriol. 1978 Mar;133(3):1383–1392. doi: 10.1128/jb.133.3.1383-1392.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aksoy S., Squires C. L., Squires C. Translational coupling of the trpB and trpA genes in the Escherichia coli tryptophan operon. J Bacteriol. 1984 Feb;157(2):363–367. doi: 10.1128/jb.157.2.363-367.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bouvier J., Pugsley A. P., Stragier P. A gene for a new lipoprotein in the dapA-purC interval of the Escherichia coli chromosome. J Bacteriol. 1991 Sep;173(17):5523–5531. doi: 10.1128/jb.173.17.5523-5531.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradshaw H. D., Jr, Traxler B. A., Minkley E. G., Jr, Nester E. W., Gordon M. P. Nucleotide sequence of the traI (helicase I) gene from the sex factor F. J Bacteriol. 1990 Jul;172(7):4127–4131. doi: 10.1128/jb.172.7.4127-4131.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chamberlain J. P. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979 Sep 15;98(1):132–135. doi: 10.1016/0003-2697(79)90716-4. [DOI] [PubMed] [Google Scholar]
  6. Chen R., Henning U. Nucleotide sequence of the gene for the peptidoglycan-associated lipoprotein of Escherichia coli K12. Eur J Biochem. 1987 Feb 16;163(1):73–77. doi: 10.1111/j.1432-1033.1987.tb10738.x. [DOI] [PubMed] [Google Scholar]
  7. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Firth N., Skurray R. Characterization of the F plasmid bifunctional conjugation gene, traG. Mol Gen Genet. 1992 Mar;232(1):145–153. doi: 10.1007/BF00299147. [DOI] [PubMed] [Google Scholar]
  9. Gennity J. M., Inouye M. The protein sequence responsible for lipoprotein membrane localization in Escherichia coli exhibits remarkable specificity. J Biol Chem. 1991 Sep 5;266(25):16458–16464. [PubMed] [Google Scholar]
  10. Ham L. M., Firth N., Skurray R. Nucleotide sequence of the F plasmid transfer gene, traH: identification of a new gene and a promoter within the transfer operon. Gene. 1989 Jan 30;75(1):157–165. doi: 10.1016/0378-1119(89)90392-2. [DOI] [PubMed] [Google Scholar]
  11. Hussain M., Ichihara S., Mizushima S. Accumulation of glyceride-containing precursor of the outer membrane lipoprotein in the cytoplasmic membrane of Escherichia coli treated with globomycin. J Biol Chem. 1980 Apr 25;255(8):3707–3712. [PubMed] [Google Scholar]
  12. Kang P. J., Craig E. A. Identification and characterization of a new Escherichia coli gene that is a dosage-dependent suppressor of a dnaK deletion mutation. J Bacteriol. 1990 Apr;172(4):2055–2064. doi: 10.1128/jb.172.4.2055-2064.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kathir P., Ippen-Ihler K. Construction and characterization of derivatives carrying insertion mutations in F plasmid transfer region genes, trbA, artA, traQ, and trbB. Plasmid. 1991 Jul;26(1):40–54. doi: 10.1016/0147-619x(91)90035-u. [DOI] [PubMed] [Google Scholar]
  14. LaRossa R. A., Van Dyk T. K. Physiological roles of the DnaK and GroE stress proteins: catalysts of protein folding or macromolecular sponges? Mol Microbiol. 1991 Mar;5(3):529–534. doi: 10.1111/j.1365-2958.1991.tb00724.x. [DOI] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Lazzaroni J. C., Portalier R. The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL). Mol Microbiol. 1992 Mar;6(6):735–742. doi: 10.1111/j.1365-2958.1992.tb01523.x. [DOI] [PubMed] [Google Scholar]
  17. Leduc M., Ishidate K., Shakibai N., Rothfield L. Interactions of Escherichia coli membrane lipoproteins with the murein sacculus. J Bacteriol. 1992 Dec;174(24):7982–7988. doi: 10.1128/jb.174.24.7982-7988.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
  19. Liu Y., Saha S., Haggård-Ljungquist E. Studies of bacteriophage P2 DNA replication. The DNA sequence of the cis-acting gene A and ori region and construction of a P2 mini-chromosome. J Mol Biol. 1993 May 20;231(2):361–374. doi: 10.1006/jmbi.1993.1288. [DOI] [PubMed] [Google Scholar]
  20. Maneewannakul K., Ippen-Ihler K. Construction and analysis of F plasmid traR, trbJ, and trbH mutants. J Bacteriol. 1993 Mar;175(5):1528–1531. doi: 10.1128/jb.175.5.1528-1531.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Miki T., Horiuchi T., Willetts N. S. Identification and characterization of four new tra cistrons on the E. coli K12 sex factor F. Plasmid. 1978 Jun;1(3):316–323. doi: 10.1016/0147-619x(78)90048-3. [DOI] [PubMed] [Google Scholar]
  22. Mizuno T. A novel peptidoglycan-associated lipoprotein (PAL) found in the outer membrane of Proteus mirabilis and other Gram-negative bacteria. J Biochem. 1981 Apr;89(4):1039–1049. [PubMed] [Google Scholar]
  23. Moore D., Hamilton C. M., Maneewannakul K., Mintz Y., Frost L. S., Ippen-Ihler K. The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin. J Bacteriol. 1993 Mar;175(5):1375–1383. doi: 10.1128/jb.175.5.1375-1383.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Moore D., Maneewannakul K., Maneewannakul S., Wu J. H., Ippen-Ihler K., Bradley D. E. Characterization of the F-plasmid conjugative transfer gene traU. J Bacteriol. 1990 Aug;172(8):4263–4270. doi: 10.1128/jb.172.8.4263-4270.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Moore D., Sowa B. A., Ippen-Ihler K. A new activity in the Ftra operon which is required for F-pilin synthesis. Mol Gen Genet. 1982;188(3):459–464. doi: 10.1007/BF00330049. [DOI] [PubMed] [Google Scholar]
  26. Moore D., Wu J. H., Kathir P., Hamilton C. M., Ippen-Ihler K. Analysis of transfer genes and gene products within the traB-traC region of the Escherichia coli fertility factor, F. J Bacteriol. 1987 Sep;169(9):3994–4002. doi: 10.1128/jb.169.9.3994-4002.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Nilsson B., Anderson S. Proper and improper folding of proteins in the cellular environment. Annu Rev Microbiol. 1991;45:607–635. doi: 10.1146/annurev.mi.45.100191.003135. [DOI] [PubMed] [Google Scholar]
  28. Orndorff P. E., Falkow S. Organization and expression of genes responsible for type 1 piliation in Escherichia coli. J Bacteriol. 1984 Aug;159(2):736–744. doi: 10.1128/jb.159.2.736-744.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Poquet I., Kornacker M. G., Pugsley A. P. The role of the lipoprotein sorting signal (aspartate +2) in pullulanase secretion. Mol Microbiol. 1993 Sep;9(5):1061–1069. doi: 10.1111/j.1365-2958.1993.tb01235.x. [DOI] [PubMed] [Google Scholar]
  31. Pugsley A. P. The complete general secretory pathway in gram-negative bacteria. Microbiol Rev. 1993 Mar;57(1):50–108. doi: 10.1128/mr.57.1.50-108.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Schandel K. A., Maneewannakul S., Vonder Haar R. A., Ippen-Ihler K., Webster R. E. Nucleotide sequence of the F plasmid gene, traC, and identification of its product. Gene. 1990 Nov 30;96(1):137–140. doi: 10.1016/0378-1119(90)90354-t. [DOI] [PubMed] [Google Scholar]
  34. Sivaprasad A. V., Jarvinen R., Puspurs A., Egan J. B. DNA replication studies with coliphage 186. III. A single phage gene is required for phage 186 replication. J Mol Biol. 1990 Jun 5;213(3):449–463. doi: 10.1016/S0022-2836(05)80207-4. [DOI] [PubMed] [Google Scholar]
  35. Tabor S., Richardson C. C. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767–4771. doi: 10.1073/pnas.84.14.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Wu J. H., Ippen-Ihler K. Nucleotide sequence of traQ and adjacent loci in the Escherichia coli K-12 F-plasmid transfer operon. J Bacteriol. 1989 Jan;171(1):213–221. doi: 10.1128/jb.171.1.213-221.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Yamaguchi K., Yu F., Inouye M. A single amino acid determinant of the membrane localization of lipoproteins in E. coli. Cell. 1988 May 6;53(3):423–432. doi: 10.1016/0092-8674(88)90162-6. [DOI] [PubMed] [Google Scholar]
  38. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  39. von Heijne G. Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. J Mol Biol. 1992 May 20;225(2):487–494. doi: 10.1016/0022-2836(92)90934-c. [DOI] [PubMed] [Google Scholar]
  40. von Heijne G. The structure of signal peptides from bacterial lipoproteins. Protein Eng. 1989 May;2(7):531–534. doi: 10.1093/protein/2.7.531. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES