Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Jan;174(1):24–29. doi: 10.1128/jb.174.1.24-29.1992

The CobII and CobIII regions of the cobalamin (vitamin B12) biosynthetic operon of Salmonella typhimurium.

J C Escalante-Semerena 1, M G Johnson 1, J R Roth 1
PMCID: PMC205671  PMID: 1729212

Abstract

A detailed deletion map of the CobII and CobIII regions of the cobalamin biosynthetic (cob) operon of Salmonella typhimurium LT2 has been constructed. The CobII region encodes functions needed for the synthesis of lower ligand 5,6-dimethylbenzimidazole (DMB); CobIII encodes functions needed for the synthesis of the nucleotide loop that joins DMB to the corrin macrocycle. The genetic analysis of 117 deletion, insertion, and point mutations indicates that (i) the CobII and CobIII mutations are contiguous--that is, they are grouped according to function; (ii) the CobII region is composed of four complementation groups (cobJKLM); (iii) cobM mutations do not complement mutations in any of the other three CobII groups; and (iv) CobIII mutations include three complementation groups that correspond to the cobU, cobS, and cobT genes.

Full text

PDF
24

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blanche F., Debussche L., Famechon A., Thibaut D., Cameron B., Crouzet J. A bifunctional protein from Pseudomonas denitrificans carries cobinamide kinase and cobinamide phosphate guanylyltransferase activities. J Bacteriol. 1991 Oct;173(19):6052–6057. doi: 10.1128/jb.173.19.6052-6057.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bochner B. R., Huang H. C., Schieven G. L., Ames B. N. Positive selection for loss of tetracycline resistance. J Bacteriol. 1980 Aug;143(2):926–933. doi: 10.1128/jb.143.2.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cameron B., Blanche F., Rouyez M. C., Bisch D., Famechon A., Couder M., Cauchois L., Thibaut D., Debussche L., Crouzet J. Genetic analysis, nucleotide sequence, and products of two Pseudomonas denitrificans cob genes encoding nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5'-phosphate) synthase. J Bacteriol. 1991 Oct;173(19):6066–6073. doi: 10.1128/jb.173.19.6066-6073.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cameron B., Guilhot C., Blanche F., Cauchois L., Rouyez M. C., Rigault S., Levy-Schil S., Crouzet J. Genetic and sequence analyses of a Pseudomonas denitrificans DNA fragment containing two cob genes. J Bacteriol. 1991 Oct;173(19):6058–6065. doi: 10.1128/jb.173.19.6058-6065.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Castilho B. A., Olfson P., Casadaban M. J. Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. J Bacteriol. 1984 May;158(2):488–495. doi: 10.1128/jb.158.2.488-495.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crouzet J., Cameron B., Cauchois L., Rigault S., Rouyez M. C., Blanche F., Thibaut D., Debussche L. Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid. J Bacteriol. 1990 Oct;172(10):5980–5990. doi: 10.1128/jb.172.10.5980-5990.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crouzet J., Cauchois L., Blanche F., Debussche L., Thibaut D., Rouyez M. C., Rigault S., Mayaux J. F., Cameron B. Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase. J Bacteriol. 1990 Oct;172(10):5968–5979. doi: 10.1128/jb.172.10.5968-5979.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Crouzet J., Levy-Schil S., Cameron B., Cauchois L., Rigault S., Rouyez M. C., Blanche F., Debussche L., Thibaut D. Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase. J Bacteriol. 1991 Oct;173(19):6074–6087. doi: 10.1128/jb.173.19.6074-6087.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Elliott T., Roth J. R. Characterization of Tn10d-Cam: a transposition-defective Tn10 specifying chloramphenicol resistance. Mol Gen Genet. 1988 Aug;213(2-3):332–338. doi: 10.1007/BF00339599. [DOI] [PubMed] [Google Scholar]
  11. Escalante-Semerena J. C., Roth J. R. Regulation of cobalamin biosynthetic operons in Salmonella typhimurium. J Bacteriol. 1987 May;169(5):2251–2258. doi: 10.1128/jb.169.5.2251-2258.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Escalante-Semerena J. C., Suh S. J., Roth J. R. cobA function is required for both de novo cobalamin biosynthesis and assimilation of exogenous corrinoids in Salmonella typhimurium. J Bacteriol. 1990 Jan;172(1):273–280. doi: 10.1128/jb.172.1.273-280.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hong J. S., Ames B. N. Localized mutagenesis of any specific small region of the bacterial chromosome. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158–3162. doi: 10.1073/pnas.68.12.3158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hughes K. T., Roth J. R. Transitory cis complementation: a method for providing transposition functions to defective transposons. Genetics. 1988 May;119(1):9–12. doi: 10.1093/genetics/119.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Höllriegl V., Lamm L., Rowold J., Hörig J., Renz P. Biosynthesis of vitamin B12. Different pathways in some aerobic and anaerobic microorganisms. Arch Microbiol. 1982 Aug;132(2):155–158. doi: 10.1007/BF00508722. [DOI] [PubMed] [Google Scholar]
  16. Hörig J. A., Renz P. Biosynthesis of vitamin B12. Some properties of the 5,6-dimethylbenzimidazole-forming system of Propionibacterium freudenreichii and Propionibacterium shermanii. Eur J Biochem. 1980 Apr;105(3):587–592. doi: 10.1111/j.1432-1033.1980.tb04536.x. [DOI] [PubMed] [Google Scholar]
  17. Hörig J. A., Renz P., Heckmann G. [5-15N]Riboflavin as precursor in the biosynthesis of the 5,6-dimethylbenzimidazole moiety of vitamin B12. A study by 1H and 15N magnetic resonance spectroscopy. J Biol Chem. 1978 Oct 25;253(20):7410–7414. [PubMed] [Google Scholar]
  18. Jeter R. M., Olivera B. M., Roth J. R. Salmonella typhimurium synthesizes cobalamin (vitamin B12) de novo under anaerobic growth conditions. J Bacteriol. 1984 Jul;159(1):206–213. doi: 10.1128/jb.159.1.206-213.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Jeter R. M., Roth J. R. Cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium. J Bacteriol. 1987 Jul;169(7):3189–3198. doi: 10.1128/jb.169.7.3189-3198.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lamm L., Heckmann G., Renz P. Biosynthesis of vitamin B12 in anaerobic bacteria. Mode of incorporation of glycine into the 5,6-dimethylbenzimidazole moiety in Eubacterium limosum. Eur J Biochem. 1982 Mar 1;122(3):569–571. [PubMed] [Google Scholar]
  21. Lamm L., Hörig J. A., Renz P., Heckmann G. Biosynthesis of vitamin B12. Experiments with the anaerobe Eubacterium limosum and some labelled substrates. Eur J Biochem. 1980 Aug;109(1):115–118. doi: 10.1111/j.1432-1033.1980.tb04775.x. [DOI] [PubMed] [Google Scholar]
  22. Maloy S. R., Nunn W. D. Selection for loss of tetracycline resistance by Escherichia coli. J Bacteriol. 1981 Feb;145(2):1110–1111. doi: 10.1128/jb.145.2.1110-1111.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Roof D. M., Roth J. R. Functions required for vitamin B12-dependent ethanolamine utilization in Salmonella typhimurium. J Bacteriol. 1989 Jun;171(6):3316–3323. doi: 10.1128/jb.171.6.3316-3323.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sanderson K. E., Roth J. R. Linkage map of Salmonella typhimurium, edition VII. Microbiol Rev. 1988 Dec;52(4):485–532. doi: 10.1128/mr.52.4.485-532.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schmieger H. A method for detection of phage mutants with altered transducing ability. Mol Gen Genet. 1971;110(4):378–381. doi: 10.1007/BF00438281. [DOI] [PubMed] [Google Scholar]
  26. Schmieger H., Backhaus H. The origin of DNA in transducing particles in P22-mutants with increased transduction-frequencies (HT-mutants). Mol Gen Genet. 1973 Jan 24;120(2):181–190. doi: 10.1007/BF00267246. [DOI] [PubMed] [Google Scholar]
  27. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  28. Vogt J. R., Lamm-Kolonko L., Renz P. Biosynthesis of vitamin B-12 in anaerobic bacteria. Experiments with Eubacterium limosum and D-erythrose 14C-labeled in different positions. Eur J Biochem. 1988 Jul 1;174(4):637–640. doi: 10.1111/j.1432-1033.1988.tb14145.x. [DOI] [PubMed] [Google Scholar]
  29. Vogt J. R., Renz P. Biosynthesis of vitamin B-12 in anaerobic bacteria. Experiments with Eubacterium limosum on the origin of the amide groups of the corrin ring and of N-3 of the 5,6-dimethylbenzimidazole part. Eur J Biochem. 1988 Feb 1;171(3):655–659. doi: 10.1111/j.1432-1033.1988.tb13836.x. [DOI] [PubMed] [Google Scholar]
  30. Way J. C., Davis M. A., Morisato D., Roberts D. E., Kleckner N. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. Gene. 1984 Dec;32(3):369–379. doi: 10.1016/0378-1119(84)90012-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES