Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Jan;174(1):331–335. doi: 10.1128/jb.174.1.331-335.1992

Synergism between the Trp repressor and Tyr repressor in repression of the aroL promoter of Escherichia coli K-12.

V M Heatwole 1, R L Somerville 1
PMCID: PMC205715  PMID: 1530846

Abstract

Computer analysis identified a potential Trp repressor operator 56 nucleotides downstream of the transcriptional start point of aroL, the gene that encodes shikimate kinase II. Tryptophan-dependent interaction of Trp repressor with this operator was demonstrated in vitro by means of a restriction endonuclease protection assay. Regulation of expression from the aroL promoter was evaluated with several genetically marked Escherichia coli strains by using a single-copy aroL-lacZ transcriptional-translational reporter system. The expression of aroL was repressed 6.9-fold by the Tyr repressor alone and 29-fold when both Tyr and Trp repressors were present. The Trp repressor had no effect on expression from the aroL promoter in the absence of the Tyr repressor. Possible mechanisms for Trp repressor-mediated repression, including cooperative interactions with the Tyr repressor, are discussed.

Full text

PDF
331

Images in this article

333Image
on p.333

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aronson B. D., Levinthal M., Somerville R. L. Activation of a cryptic pathway for threonine metabolism via specific IS3-mediated alteration of promoter structure in Escherichia coli. J Bacteriol. 1989 Oct;171(10):5503–5511. doi: 10.1128/jb.171.10.5503-5511.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bass S., Sorrells V., Youderian P. Mutant Trp repressors with new DNA-binding specificities. Science. 1988 Oct 14;242(4876):240–245. doi: 10.1126/science.3140377. [DOI] [PubMed] [Google Scholar]
  4. Berman M. L., Jackson D. E. Selection of lac gene fusions in vivo: ompR-lacZ fusions that define a functional domain of the ompR gene product. J Bacteriol. 1984 Aug;159(2):750–756. doi: 10.1128/jb.159.2.750-756.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collado-Vides J., Magasanik B., Gralla J. D. Control site location and transcriptional regulation in Escherichia coli. Microbiol Rev. 1991 Sep;55(3):371–394. doi: 10.1128/mr.55.3.371-394.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DeFeyter R. C., Davidson B. E., Pittard J. Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12. J Bacteriol. 1986 Jan;165(1):233–239. doi: 10.1128/jb.165.1.233-239.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DeFeyter R. C., Pittard J. Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12. J Bacteriol. 1986 Jan;165(1):226–232. doi: 10.1128/jb.165.1.226-232.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Elledge S. J., Davis R. W. Position and density effects on repression by stationary and mobile DNA-binding proteins. Genes Dev. 1989 Feb;3(2):185–197. doi: 10.1101/gad.3.2.185. [DOI] [PubMed] [Google Scholar]
  10. Ely B., Pittard J. Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12. J Bacteriol. 1979 Jun;138(3):933–943. doi: 10.1128/jb.138.3.933-943.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Golding A., Weickert M. J., Tokeson J. P., Garges S., Adhya S. A mutation defining ultrainduction of the Escherichia coli gal operon. J Bacteriol. 1991 Oct;173(19):6294–6296. doi: 10.1128/jb.173.19.6294-6296.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Heatwole V. M., Somerville R. L. Cloning, nucleotide sequence, and characterization of mtr, the structural gene for a tryptophan-specific permease of Escherichia coli K-12. J Bacteriol. 1991 Jan;173(1):108–115. doi: 10.1128/jb.173.1.108-115.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Heatwole V. M., Somerville R. L. The tryptophan-specific permease gene, mtr, is differentially regulated by the tryptophan and tyrosine repressors in Escherichia coli K-12. J Bacteriol. 1991 Jun;173(11):3601–3604. doi: 10.1128/jb.173.11.3601-3604.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lanzer M., Bujard H. Promoters largely determine the efficiency of repressor action. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8973–8977. doi: 10.1073/pnas.85.23.8973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Millar G., Lewendon A., Hunter M. G., Coggins J. R. The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product. Biochem J. 1986 Jul 15;237(2):427–437. doi: 10.1042/bj2370427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Muday G. K., Johnson D. I., Somerville R. L., Herrmann K. M. The tyrosine repressor negatively regulates aroH expression in Escherichia coli. J Bacteriol. 1991 Jun;173(12):3930–3932. doi: 10.1128/jb.173.12.3930-3932.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pittard A. J., Davidson B. E. TyrR protein of Escherichia coli and its role as repressor and activator. Mol Microbiol. 1991 Jul;5(7):1585–1592. doi: 10.1111/j.1365-2958.1991.tb01904.x. [DOI] [PubMed] [Google Scholar]
  19. Rex J. H., Aronson B. D., Somerville R. L. The tdh and serA operons of Escherichia coli: mutational analysis of the regulatory elements of leucine-responsive genes. J Bacteriol. 1991 Oct;173(19):5944–5953. doi: 10.1128/jb.173.19.5944-5953.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sarsero J. P., Wookey P. J., Pittard A. J. Regulation of expression of the Escherichia coli K-12 mtr gene by TyrR protein and Trp repressor. J Bacteriol. 1991 Jul;173(13):4133–4143. doi: 10.1128/jb.173.13.4133-4143.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  22. Wanner B. L. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12. J Mol Biol. 1986 Sep 5;191(1):39–58. doi: 10.1016/0022-2836(86)90421-3. [DOI] [PubMed] [Google Scholar]
  23. Yu X. M., Reznikoff W. S. Deletion analysis of the CAP-cAMP binding site of the Escherichia coli lactose promoter. Nucleic Acids Res. 1984 Jul 11;12(13):5449–5464. doi: 10.1093/nar/12.13.5449. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES