Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Jan;174(2):627–629. doi: 10.1128/jb.174.2.627-629.1992

The gene for a 4.5S RNA homolog from Mycoplasma pneumoniae: genetic selection, sequence, and transcription analysis.

P Simoneau 1, P C Hu 1
PMCID: PMC205759  PMID: 1370291

Abstract

In an effort to make an inventory of the tRNA genes of Mycoplasma pneumoniae, a DNA fragment was found to contain a sequence that can be folded into a hairpin structure very similar to that of the 4.5S RNA of Escherichia coli. Recombinant plasmids carrying this region were able to complement E. coli strains that were deficient in 4.5S RNA. S1 mapping showed that the mature transcript is only 79 nucleotides long.

Full text

PDF
627

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brennwald P., Liao X., Holm K., Porter G., Wise J. A. Identification of an essential Schizosaccharomyces pombe RNA homologous to the 7SL component of signal recognition particle. Mol Cell Biol. 1988 Apr;8(4):1580–1590. doi: 10.1128/mcb.8.4.1580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown S., Fournier M. J. The 4.5 S RNA gene of Escherichia coli is essential for cell growth. J Mol Biol. 1984 Sep 25;178(3):533–550. doi: 10.1016/0022-2836(84)90237-7. [DOI] [PubMed] [Google Scholar]
  3. Brown S. Genes for 7S RNAs can replace the gene for 4.5S RNA in growth of Escherichia coli. J Bacteriol. 1991 Mar;173(5):1835–1837. doi: 10.1128/jb.173.5.1835-1837.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown S. Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli. Cell. 1987 Jun 19;49(6):825–833. doi: 10.1016/0092-8674(87)90620-9. [DOI] [PubMed] [Google Scholar]
  5. Brown S., Thon G., Tolentino E. Genetic selection and DNA sequences of 4.5S RNA homologs. J Bacteriol. 1989 Dec;171(12):6517–6520. doi: 10.1128/jb.171.12.6517-6520.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brown S. Time of action of 4.5 S RNA in Escherichia coli translation. J Mol Biol. 1989 Sep 5;209(1):79–90. doi: 10.1016/0022-2836(89)90171-x. [DOI] [PubMed] [Google Scholar]
  7. Colman S. D., Hu P. C., Litaker W., Bott K. F. A physical map of the Mycoplasma genitalium genome. Mol Microbiol. 1990 Apr;4(4):683–687. doi: 10.1111/j.1365-2958.1990.tb00638.x. [DOI] [PubMed] [Google Scholar]
  8. Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
  9. Hsu L. M., Zagorski J., Fournier M. J. Cloning and sequence analysis of the Escherichia coli 4.5 S RNA gene. J Mol Biol. 1984 Sep 25;178(3):509–531. doi: 10.1016/0022-2836(84)90236-5. [DOI] [PubMed] [Google Scholar]
  10. Hu P. C., Cole R. M., Huang Y. S., Graham J. A., Gardner D. E., Collier A. M., Clyde W. A., Jr Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science. 1982 Apr 16;216(4543):313–315. doi: 10.1126/science.6801766. [DOI] [PubMed] [Google Scholar]
  11. Inamine J. M., Ho K. C., Loechel S., Hu P. C. Evidence that UGA is read as a tryptophan codon rather than as a stop codon by Mycoplasma pneumoniae, Mycoplasma genitalium, and Mycoplasma gallisepticum. J Bacteriol. 1990 Jan;172(1):504–506. doi: 10.1128/jb.172.1.504-506.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Inouye M., Delihas N. Small RNAs in the prokaryotes: a growing list of diverse roles. Cell. 1988 Apr 8;53(1):5–7. doi: 10.1016/0092-8674(88)90480-1. [DOI] [PubMed] [Google Scholar]
  13. Kaine B. P. Structure of the archaebacterial 7S RNA molecule. Mol Gen Genet. 1990 May;221(3):315–321. doi: 10.1007/BF00259394. [DOI] [PubMed] [Google Scholar]
  14. Moritz A., Goebel W. Characterization of the 7S RNA and its gene from halobacteria. Nucleic Acids Res. 1985 Oct 11;13(19):6969–6979. doi: 10.1093/nar/13.19.6969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. doi: 10.1016/0378-1119(83)90040-9. [DOI] [PubMed] [Google Scholar]
  16. Poritz M. A., Bernstein H. D., Strub K., Zopf D., Wilhelm H., Walter P. An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle. Science. 1990 Nov 23;250(4984):1111–1117. doi: 10.1126/science.1701272. [DOI] [PubMed] [Google Scholar]
  17. Poritz M. A., Strub K., Walter P. Human SRP RNA and E. coli 4.5S RNA contain a highly homologous structural domain. Cell. 1988 Oct 7;55(1):4–6. doi: 10.1016/0092-8674(88)90003-7. [DOI] [PubMed] [Google Scholar]
  18. Samuelsson T., Guindy Y. Nucleotide sequence of a Mycoplasma mycoides RNA which is homologous to E. coli 4.5S RNA. Nucleic Acids Res. 1990 Aug 25;18(16):4938–4938. doi: 10.1093/nar/18.16.4938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Simoneau P., Wenzel R., Herrmann R., Hu P. C. Nucleotide sequence of a tRNA cluster from Mycoplasma pneumoniae. Nucleic Acids Res. 1990 May 11;18(9):2814–2814. doi: 10.1093/nar/18.9.2814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Soberon X., Covarrubias L., Bolivar F. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene. 1980 May;9(3-4):287–305. doi: 10.1016/0378-1119(90)90328-o. [DOI] [PubMed] [Google Scholar]
  22. Struck J. C., Toschka H. Y., Specht T., Erdmann V. A. Common structural features between eukaryotic 7SL RNAs, eubacterial 4.5S RNA and scRNA and archaebacterial 7S RNA. Nucleic Acids Res. 1988 Aug 11;16(15):7740–7740. doi: 10.1093/nar/16.15.7740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Walter P., Gilmore R., Blobel G. Protein translocation across the endoplasmic reticulum. Cell. 1984 Aug;38(1):5–8. doi: 10.1016/0092-8674(84)90520-8. [DOI] [PubMed] [Google Scholar]
  24. Wenzel R., Herrmann R. Cloning of the complete Mycoplasma pneumoniae genome. Nucleic Acids Res. 1989 Sep 12;17(17):7029–7043. doi: 10.1093/nar/17.17.7029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES