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. 1992 Mar;174(6):2039–2042. doi: 10.1128/jb.174.6.2039-2042.1992

Differential depolymerization mechanisms of pectate lyases secreted by Erwinia chrysanthemi EC16.

J F Preston 3rd 1, J D Rice 1, L O Ingram 1, N T Keen 1
PMCID: PMC205812  PMID: 1548242

Abstract

The four pectate lyases (EC 4.2.2.2) secreted by Erwinia chrysanthemi EC16 have been individually produced as recombinant enzymes in Escherichia coli. Oligogalacturonates formed from polygalacturonic acid during reactions catalyzed by each enzyme have been determined by high-performance liquid chromatography analysis. PLa catalyzes the formation of a series of oligomers ranging from dimer to dodecamer through a random endolytic depolarization mechanism. PLb and PLc are trimer- and tetramer-generating enzymes with an identical combination of endolytic and exolytic mechanisms. PLe catalyzes a nonrandom endolytic depolymerization with the formation of dimer as the predominant product. The pectate lyases secreted by E. chrysanthemi EC16 represent a battery of enzymes with three distinct approaches to the depolymerization of plant cell walls.

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Selected References

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  1. Barras F., Thurn K. K., Chatterjee A. K. Resolution of four pectate lyase structural genes of Erwinia chrysanthemi (EC16) and characterization of the enzymes produced in Escherichia coli. Mol Gen Genet. 1987 Sep;209(2):319–325. doi: 10.1007/BF00329660. [DOI] [PubMed] [Google Scholar]
  2. Chatterjee A. K., Starr M. P. Genetics of Erwinia species. Annu Rev Microbiol. 1980;34:645–676. doi: 10.1146/annurev.mi.34.100180.003241. [DOI] [PubMed] [Google Scholar]
  3. Dahler G. S., Barras F., Keen N. T. Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16. J Bacteriol. 1990 Oct;172(10):5803–5815. doi: 10.1128/jb.172.10.5803-5815.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. He S. Y., Collmer A. Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthemi EC16. J Bacteriol. 1990 Sep;172(9):4988–4995. doi: 10.1128/jb.172.9.4988-4995.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Keen N. T., Tamaki S. Structure of two pectate lyase genes from Erwinia chrysanthemi EC16 and their high-level expression in Escherichia coli. J Bacteriol. 1986 Nov;168(2):595–606. doi: 10.1128/jb.168.2.595-606.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Tamaki S. J., Gold S., Robeson M., Manulis S., Keen N. T. Structure and organization of the pel genes from Erwinia chrysanthemi EC16. J Bacteriol. 1988 Aug;170(8):3468–3478. doi: 10.1128/jb.170.8.3468-3478.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wandersman C., Delepelaire P., Letoffe S., Schwartz M. Characterization of Erwinia chrysanthemi extracellular proteases: cloning and expression of the protease genes in Escherichia coli. J Bacteriol. 1987 Nov;169(11):5046–5053. doi: 10.1128/jb.169.11.5046-5053.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Witholt B., Boekhout M., Brock M., Kingma J., Heerikhuizen H. V., Leij L. D. An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli. Anal Biochem. 1976 Jul;74(1):160–170. doi: 10.1016/0003-2697(76)90320-1. [DOI] [PubMed] [Google Scholar]

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