Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Apr;174(7):2172–2177. doi: 10.1128/jb.174.7.2172-2177.1992

Capsule structure of Proteus mirabilis (ATCC 49565).

L M Beynon 1, A J Dumanski 1, R J McLean 1, L L MacLean 1, J C Richards 1, M B Perry 1
PMCID: PMC205835  PMID: 1551839

Abstract

Proteus mirabilis 2573 (ATCC 49565) produces an acidic capsular polysaccharide which was shown from glycose analysis, carboxyl reduction, methylation, periodate oxidation, and the application of one dimensional and two-dimensional high-resolution nuclear magnetic resonance techniques to be a high-molecular-weight polymer of branched trisaccharide units composed of 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine), 2-acetamido-2,6-dideoxy-L-galactose (N-acetyl-L-fucosamine), and D-glucuronic acid, having the structure: [formula: see text] P. mirabilis 2573 also produces an O:6 serotype lipopolysaccharide in which the O-chain component has the same structure as the homologous capsular polysaccharide. This is the first report of a defined capsular polysaccharide in this bacterial genus.

Full text

PDF
2172

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beynon L. M., Perry M. B., Richards J. C. Structure of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 7. Carbohydr Res. 1991 Jan 15;209:211–223. doi: 10.1016/0008-6215(91)80158-j. [DOI] [PubMed] [Google Scholar]
  2. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. doi: 10.1016/0003-2697(73)90377-1. [DOI] [PubMed] [Google Scholar]
  3. Gatt R., Berman E. R. A rapid procedure for the estimation of amino sugars on a micro scale. Anal Biochem. 1966 Apr;15(1):167–171. doi: 10.1016/0003-2697(66)90262-4. [DOI] [PubMed] [Google Scholar]
  4. Goldman R. C., White D., Orskov F., Orskov I., Rick P. D., Lewis M. S., Bhattacharjee A. K., Leive L. A surface polysaccharide of Escherichia coli O111 contains O-antigen and inhibits agglutination of cells by O-antiserum. J Bacteriol. 1982 Sep;151(3):1210–1221. doi: 10.1128/jb.151.3.1210-1221.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graham L. L., Harris R., Villiger W., Beveridge T. J. Freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles. J Bacteriol. 1991 Mar;173(5):1623–1633. doi: 10.1128/jb.173.5.1623-1633.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Griffith D. P., Musher D. M., Itin C. Urease. The primary cause of infection-induced urinary stones. Invest Urol. 1976 Mar;13(5):346–350. [PubMed] [Google Scholar]
  7. Johnson K. G., Perry M. B. Improved techniques for the preparation of bacterial lipopolysaccharides. Can J Microbiol. 1976 Jan;22(1):29–34. doi: 10.1139/m76-004. [DOI] [PubMed] [Google Scholar]
  8. Kumar A., Ernst R. R., Wüthrich K. A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules. Biochem Biophys Res Commun. 1980 Jul 16;95(1):1–6. doi: 10.1016/0006-291x(80)90695-6. [DOI] [PubMed] [Google Scholar]
  9. Mackie E. B., Brown K. N., Lam J., Costerton J. W. Morphological stabilization of capsules of group B streptococci, types Ia, Ib, II, and III, with specific antibody. J Bacteriol. 1979 May;138(2):609–617. doi: 10.1128/jb.138.2.609-617.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McLean R. J., Lawrence J. R., Korber D. R., Caldwell D. E. Proteus mirabilis biofilm protection against struvite crystal dissolution and its implications in struvite urolithiasis. J Urol. 1991 Oct;146(4):1138–1142. doi: 10.1016/s0022-5347(17)38026-6. [DOI] [PubMed] [Google Scholar]
  11. McLean R. J., Nickel J. C., Cheng K. J., Costerton J. W. The ecology and pathogenicity of urease-producing bacteria in the urinary tract. Crit Rev Microbiol. 1988;16(1):37–79. doi: 10.3109/10408418809104467. [DOI] [PubMed] [Google Scholar]
  12. McLean R. J., Nickel J. C., Noakes V. C., Costerton J. W. An in vitro ultrastructural study of infectious kidney stone genesis. Infect Immun. 1985 Sep;49(3):805–811. doi: 10.1128/iai.49.3.805-811.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Penner J. L., Hennessy J. N. Separate O-grouping schemes for serotyping clinical isolates of Proteus vulgaris and Proteus mirabilis. J Clin Microbiol. 1980 Sep;12(3):304–309. doi: 10.1128/jcm.12.3.304-309.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stahl S. J., Stewart K. R., Williams F. D. Extracellular slime associated with Proteus mirabilis during swarming. J Bacteriol. 1983 May;154(2):930–937. doi: 10.1128/jb.154.2.930-937.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES