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. 1992 Apr;174(7):2193–2198. doi: 10.1128/jb.174.7.2193-2198.1992

The phylogeny of marine and freshwater caulobacters reflects their habitat.

D A Stahl 1, R Key 1, B Flesher 1, J Smit 1
PMCID: PMC205838  PMID: 1551840

Abstract

Caulobacter is a distinctive genus of prosthecate bacteria. Because caulobacters adhere to surfaces and are found in diverse locales, their role in oligotrophic environments and bacterial biofilm communities is of interest. The phylogenetic relationships of a group of marine and freshwater caulobacters were examined in part to address whether the taxonomic grouping of these bacteria (based primarily on morphological characters) was consistent with 16S rRNA sequence divergence. The caulobacters examined (9 marine and 11 freshwater species or strains) were affiliated with the alpha proteobacteria. They made up a diverse yet, with the possible exception of a strain of Caulobacter subvibrioides, coherent assemblage. The diversity was most apparent in a comparison of freshwater and marine isolates; an early divergence within the main caulobacter lineage generally corresponded to strains isolated from freshwater and marine habitats. The marine caulobacter assemblage was not exclusive; it also embraced strains of marine hyphomonads and Rhodobacter capsulatus. We hypothesize that these genera are derived from more ancestral caulobacters. Overall, the data are consistent with the interpretation that all of the caulobacters examined, with the possible exception of C. subvibrioides, are ancestrally related, albeit anciently, and that most often division by terrestrial and marine habitats corresponds to an early evolutionary divergence within the genus.

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Selected References

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  1. Amann R. I., Krumholz L., Stahl D. A. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol. 1990 Feb;172(2):762–770. doi: 10.1128/jb.172.2.762-770.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anast Nick, Smit John. Isolation and Characterization of Marine Caulobacters and Assessment of Their Potential for Genetic Experimentation. Appl Environ Microbiol. 1988 Mar;54(3):809–817. doi: 10.1128/aem.54.3.809-817.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DeLong E. F., Wickham G. S., Pace N. R. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. Science. 1989 Mar 10;243(4896):1360–1363. doi: 10.1126/science.2466341. [DOI] [PubMed] [Google Scholar]
  4. Devereux R., He S. H., Doyle C. L., Orkland S., Stahl D. A., LeGall J., Whitman W. B. Diversity and origin of Desulfovibrio species: phylogenetic definition of a family. J Bacteriol. 1990 Jul;172(7):3609–3619. doi: 10.1128/jb.172.7.3609-3619.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ely B., Gerardot C. J. Use of pulsed-field-gradient gel electrophoresis to construct a physical map of the Caulobacter crescentus genome. Gene. 1988 Sep 7;68(2):323–333. doi: 10.1016/0378-1119(88)90035-2. [DOI] [PubMed] [Google Scholar]
  6. Gilchrist A., Smit J. Transformation of freshwater and marine caulobacters by electroporation. J Bacteriol. 1991 Jan;173(2):921–925. doi: 10.1128/jb.173.2.921-925.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Giovannoni S. J., Britschgi T. B., Moyer C. L., Field K. G. Genetic diversity in Sargasso Sea bacterioplankton. Nature. 1990 May 3;345(6270):60–63. doi: 10.1038/345060a0. [DOI] [PubMed] [Google Scholar]
  8. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6955–6959. doi: 10.1073/pnas.82.20.6955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee S., Fuhrman J. A. DNA hybridization to compare species compositions of natural bacterioplankton assemblages. Appl Environ Microbiol. 1990 Mar;56(3):739–746. doi: 10.1128/aem.56.3.739-746.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MacRae J. D., Smit J. Characterization of caulobacters isolated from wastewater treatment systems. Appl Environ Microbiol. 1991 Mar;57(3):751–758. doi: 10.1128/aem.57.3.751-758.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Merker R. I., Smit J. Characterization of the adhesive holdfast of marine and freshwater caulobacters. Appl Environ Microbiol. 1988 Aug;54(8):2078–2085. doi: 10.1128/aem.54.8.2078-2085.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nikitin D. I., Vishnewetskaya OYu, Chumakov K. M., Zlatkin I. V. Evolutionary relationship of some stalked and budding bacteria (genera Caulobacter, "Hyphobacter", Hyphomonas and Hyphomicrobium) as studied by the new integral taxonomical method. Arch Microbiol. 1990;153(2):123–128. doi: 10.1007/BF00247808. [DOI] [PubMed] [Google Scholar]
  13. Olsen G. J., Larsen N., Woese C. R. The ribosomal RNA database project. Nucleic Acids Res. 1991 Apr 25;19 (Suppl):2017–2021. doi: 10.1093/nar/19.suppl.2017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. POINDEXTER J. S. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP. Bacteriol Rev. 1964 Sep;28:231–295. doi: 10.1128/br.28.3.231-295.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pace B., Matthews E. A., Johnson K. D., Cantor C. R., Pace N. R. Conserved 5S rRNA complement to tRNA is not required for protein synthesis. Proc Natl Acad Sci U S A. 1982 Jan;79(1):36–40. doi: 10.1073/pnas.79.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Poindexter J. S. The caulobacters: ubiquitous unusual bacteria. Microbiol Rev. 1981 Mar;45(1):123–179. doi: 10.1128/mr.45.1.123-179.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shapiro L. Generation of polarity during Caulobacter cell differentiation. Annu Rev Cell Biol. 1985;1:173–207. doi: 10.1146/annurev.cb.01.110185.001133. [DOI] [PubMed] [Google Scholar]
  18. Smit J., Agabian N. Cloning of the major protein of the Caulobacter crescentus periodic surface layer: detection and characterization of the cloned peptide by protein expression assays. J Bacteriol. 1984 Dec;160(3):1137–1145. doi: 10.1128/jb.160.3.1137-1145.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Smit J., Grano D. A., Glaeser R. M., Agabian N. Periodic surface array in Caulobacter crescentus: fine structure and chemical analysis. J Bacteriol. 1981 Jun;146(3):1135–1150. doi: 10.1128/jb.146.3.1135-1150.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stackebrandt E., Fischer A., Roggentin T., Wehmeyer U., Bomar D., Smida J. A phylogenetic survey of budding, and/or prosthecate, non-phototrophic eubacteria: membership of Hyphomicrobium, Hyphomonas, Pedomicrobium, Filomicrobium, Caulobacter and "dichotomicrobium" to the alpha-subdivision of purple non-sulfur bacteria. Arch Microbiol. 1988;149(6):547–556. doi: 10.1007/BF00446759. [DOI] [PubMed] [Google Scholar]
  21. Walker S. G., Smith S. H., Smit J. Isolation and comparison of the paracrystalline surface layer proteins of freshwater caulobacters. J Bacteriol. 1992 Mar;174(6):1783–1792. doi: 10.1128/jb.174.6.1783-1792.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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