Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Apr;174(7):2215–2221. doi: 10.1128/jb.174.7.2215-2221.1992

Diauxic growth of Agrobacterium tumefaciens 15955 on succinate and mannopine.

C S Nautiyal 1, P Dion 1, W S Chilton 1
PMCID: PMC205841  PMID: 1551843

Abstract

Diauxic growth was observed upon incubation of Agrobacterium tumefaciens 15955 on a mixture of succinate and mannopine as the carbon source. Diauxic growth was also observed when either fumarate or L-malate was mixed with mannopine. No diauxie was detectable when A. tumefaciens 15955 was grown on a mixture of mannopine and glucose, fructose, sucrose, or L-arabinose. Preferential utilization of succinate was observed in the initial growth phase of diauxie, whereas the final growth phase occurred at the expense of mannopine. Cells harvested during the initial growth phase exhibited a capacity for uptake of [14C]succinate but not of [14C] mannopine. A capacity for [14C]mannopine uptake was expressed during the final growth phase. Extracts from cells grown on a mixture of succinate and mannopine exhibited a low level of mannopine cyclase activity in the initial phase of diauxie. This activity increased substantially in the final phase of growth. Added succinate had no effect on the rate of [14C]mannopine uptake or mannopine cyclase activities of cells previously grown on mannopine. Diauxie was also observed during growth of strain 15955 on a mixture of succinate and octopine.

Full text

PDF
2215

Images in this article

2219Image
on p.2219

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang C. C., Chen C. M., Adams B. R., Trost B. M. Leucinopine, a characteristic compound of some crown-gall tumors. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3573–3576. doi: 10.1073/pnas.80.12.3573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chilton W. S., Chilton M. D. Mannityl opine analogs allow isolation of catabolic pathway regulatory mutants. J Bacteriol. 1984 May;158(2):650–658. doi: 10.1128/jb.158.2.650-658.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dessaux Y., Guyon P., Farrand S. K., Petit A., Tempé J. Agrobacterium Ti and Ri plasmids specify enzymic lactonization of mannopine to agropine. J Gen Microbiol. 1986 Sep;132(9):2549–2559. doi: 10.1099/00221287-132-9-2549. [DOI] [PubMed] [Google Scholar]
  4. Dessaux Y., Guyon P., Petit A., Tempé J., Demarez M., Legrain C., Tate M. E., Farrand S. K. Opine utilization by Agrobacterium spp.: octopine-type Ti plasmids encode two pathways for mannopinic acid degradation. J Bacteriol. 1988 Jul;170(7):2939–2946. doi: 10.1128/jb.170.7.2939-2946.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Finan T. M., Wood J. M., Jordan D. C. Succinate transport in Rhizobium leguminosarum. J Bacteriol. 1981 Oct;148(1):193–202. doi: 10.1128/jb.148.1.193-202.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. George S. E., Costenbader C. J., Melton T. Diauxic growth in Azotobacter vinelandii. J Bacteriol. 1985 Nov;164(2):866–871. doi: 10.1128/jb.164.2.866-871.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guyon P., Chilton M. D., Petit A., Tempé J. Agropine in "null-type" crown gall tumors: Evidence for generality of the opine concept. Proc Natl Acad Sci U S A. 1980 May;77(5):2693–2697. doi: 10.1073/pnas.77.5.2693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harder W., Dijkhuizen L. Strategies of mixed substrate utilization in microorganisms. Philos Trans R Soc Lond B Biol Sci. 1982 Jun 11;297(1088):459–480. doi: 10.1098/rstb.1982.0055. [DOI] [PubMed] [Google Scholar]
  9. Krulwich T. A., Ensign J. C. Alteration of glucose metabolism of Arthrobacter crystallopoietes by compounds which induce sphere to rod morphogenesis. J Bacteriol. 1969 Feb;97(2):526–534. doi: 10.1128/jb.97.2.526-534.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee I. H., Fredrickson A. G., Tsuchiya H. M. Diauxic growth of Propionibacterium shermanii. Appl Microbiol. 1974 Nov;28(5):831–835. doi: 10.1128/am.28.5.831-835.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lessie T. G., Phibbs P. V., Jr Alternative pathways of carbohydrate utilization in pseudomonads. Annu Rev Microbiol. 1984;38:359–388. doi: 10.1146/annurev.mi.38.100184.002043. [DOI] [PubMed] [Google Scholar]
  12. Lynch W. H., Franklin M. Effect of temperature on diauxic growth with glucose and organic acids in Pseudomonas fluorescens. Arch Microbiol. 1978 Aug 1;118(2):133–140. doi: 10.1007/BF00415721. [DOI] [PubMed] [Google Scholar]
  13. McKenney D., Melton T. Isolation and characterization of ack and pta mutations in Azotobacter vinelandii affecting acetate-glucose diauxie. J Bacteriol. 1986 Jan;165(1):6–12. doi: 10.1128/jb.165.1.6-12.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nautiyal C. S., Dion P., Chilton W. S. Mannopine and mannopinic acid as substrates for Arthrobacter sp. strain MBA209 and Pseudomonas putida NA513. J Bacteriol. 1991 May;173(9):2833–2841. doi: 10.1128/jb.173.9.2833-2841.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ronson C. W., Lyttleton P., Robertson J. G. C(4)-dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4284–4288. doi: 10.1073/pnas.78.7.4284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tauchert K., Jahn A., Oelze J. Control of diauxic growth of Azotobacter vinelandii on acetate and glucose. J Bacteriol. 1990 Nov;172(11):6447–6451. doi: 10.1128/jb.172.11.6447-6451.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ucker D. S., Signer E. R. Catabolite-repression-like phenomenon in Rhizobium meliloti. J Bacteriol. 1978 Dec;136(3):1197–1200. doi: 10.1128/jb.136.3.1197-1200.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES