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. 1992 Apr;174(8):2694–2701. doi: 10.1128/jb.174.8.2694-2701.1992

Sequence analysis and complementation studies of the argJ gene encoding ornithine acetyltransferase from Neisseria gonorrhoeae.

P R Martin 1, M H Mulks 1
PMCID: PMC205910  PMID: 1339419

Abstract

Clinical isolates of Neisseria gonorrhoeae frequently are deficient in arginine biosynthesis. These auxotrophs often have defects in the fifth step of the arginine biosynthetic pathway, the conversion of acetylornithine to ornithine. This reaction is catalyzed by the enzyme ornithine acetyltransferase, which is a product of the argJ gene. We have cloned and sequenced the gonococcal argJ gene and found that it contains an open reading frame of 1,218 nucleotides and encodes a peptide with a deduced Mr of 42,879. This predicted size was supported by minicell analysis. This gene was capable of complementing both Escherichia coli argE and argA mutations and of transforming an ArgJ- strain of N. gonorrhoeae to Arg+. Southern blots were able to detect bands that specifically hybridized to the gonococcal argJ gene in genomic DNA from Pseudomonas aeruginosa but not E. coli, a result that reflects the divergent nature of the arginine biosynthetic pathway in these organisms.

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Selected References

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  1. Brown K., Finch P. W., Hickson I. D., Emmerson P. T. Complete nucleotide sequence of the Escherichia coli argA gene. Nucleic Acids Res. 1987 Dec 23;15(24):10586–10586. doi: 10.1093/nar/15.24.10586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carifo K., Catlin B. W. Neisseria gonorrhoeae auxotyping: differentiation of clinical isolates based on growth responses on chemically defined media. Appl Microbiol. 1973 Sep;26(3):223–230. doi: 10.1128/am.26.3.223-230.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cunin R., Eckhardt T., Piette J., Boyen A., Piérard A., Glansdorff N. Molecular basis for modulated regulation of gene expression in the arginine regulon of Escherichia coli K-12. Nucleic Acids Res. 1983 Aug 11;11(15):5007–5019. doi: 10.1093/nar/11.15.5007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cunin R., Glansdorff N., Piérard A., Stalon V. Biosynthesis and metabolism of arginine in bacteria. Microbiol Rev. 1986 Sep;50(3):314–352. doi: 10.1128/mr.50.3.314-352.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goodman S. D., Scocca J. J. Identification and arrangement of the DNA sequence recognized in specific transformation of Neisseria gonorrhoeae. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6982–6986. doi: 10.1073/pnas.85.18.6982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gouy M., Gautier C. Codon usage in bacteria: correlation with gene expressivity. Nucleic Acids Res. 1982 Nov 25;10(22):7055–7074. doi: 10.1093/nar/10.22.7055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grosjean H., Fiers W. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. Gene. 1982 Jun;18(3):199–209. doi: 10.1016/0378-1119(82)90157-3. [DOI] [PubMed] [Google Scholar]
  9. Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoare D. S., Hoare S. L. Feedback regulation of arginine biosynthesis in blue-green algae and photosynthetic bacteria. J Bacteriol. 1966 Aug;92(2):375–379. doi: 10.1128/jb.92.2.375-379.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Knapp J. S., Holmes K. K. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis. 1975 Aug;132(2):204–208. doi: 10.1093/infdis/132.2.204. [DOI] [PubMed] [Google Scholar]
  12. Knapp J. S., Tam M. R., Nowinski R. C., Holmes K. K., Sandström E. G. Serological classification of Neisseria gonorrhoeae with use of monoclonal antibodies to gonococcal outer membrane protein I. J Infect Dis. 1984 Jul;150(1):44–48. doi: 10.1093/infdis/150.1.44. [DOI] [PubMed] [Google Scholar]
  13. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  14. Martin P. R., Cooperider J. W., Mulks M. H. Sequence of the argF gene encoding ornithine transcarbamoylase from Neisseria gonorrhoeae. Gene. 1990 Sep 28;94(1):139–140. doi: 10.1016/0378-1119(90)90482-7. [DOI] [PubMed] [Google Scholar]
  15. Mayer L. W., Schoolnik G. K., Falkow S. Genetic studies on Neisseria gonorrhoeae from disseminated gonococcal infections. Infect Immun. 1977 Oct;18(1):165–172. doi: 10.1128/iai.18.1.165-172.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Picard F. J., Dillon J. R. Cloning and organization of seven arginine biosynthesis genes from Neisseria gonorrhoeae. J Bacteriol. 1989 Mar;171(3):1644–1651. doi: 10.1128/jb.171.3.1644-1651.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pohlner J., Halter R., Beyreuther K., Meyer T. F. Gene structure and extracellular secretion of Neisseria gonorrhoeae IgA protease. 1987 Jan 29-Feb 4Nature. 325(6103):458–462. doi: 10.1038/325458a0. [DOI] [PubMed] [Google Scholar]
  18. Rossau R., Heyndrickx L., Van Heuverswyn H. Nucleotide sequence of a 16S ribosomal RNA gene from Neisseria gonorrhoeae. Nucleic Acids Res. 1988 Jul 11;16(13):6227–6227. doi: 10.1093/nar/16.13.6227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shinners E. N., Catlin B. W. Arginine biosynthesis in Neisseria gonorrhoeae: enzymes catalyzing the formation of ornithine and citrulline. J Bacteriol. 1978 Oct;136(1):131–135. doi: 10.1128/jb.136.1.131-135.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith M. C., Czaplewski L., North A. K., Baumberg S., Stockley P. G. Sequences required for regulation of arginine biosynthesis promoters are conserved between Bacillus subtilis and Escherichia coli. Mol Microbiol. 1989 Jan;3(1):23–28. doi: 10.1111/j.1365-2958.1989.tb00099.x. [DOI] [PubMed] [Google Scholar]
  22. Smith M. C., Mountain A., Baumberg S. Cloning in Escherichia coli of a Bacillus subtilis arginine repressor gene through its ability to confer structural stability on a fragment carrying genes of arginine biosynthesis. Mol Gen Genet. 1986 Oct;205(1):176–182. doi: 10.1007/BF02428049. [DOI] [PubMed] [Google Scholar]
  23. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  24. Stern A., Brown M., Nickel P., Meyer T. F. Opacity genes in Neisseria gonorrhoeae: control of phase and antigenic variation. Cell. 1986 Oct 10;47(1):61–71. doi: 10.1016/0092-8674(86)90366-1. [DOI] [PubMed] [Google Scholar]
  25. Thöny B., Hennecke H. The -24/-12 promoter comes of age. FEMS Microbiol Rev. 1989 Dec;5(4):341–357. doi: 10.1016/0168-6445(89)90028-4. [DOI] [PubMed] [Google Scholar]
  26. Udaka S. Pathway-specific pattern of control of arginine biosynthesis in bacteria. J Bacteriol. 1966 Feb;91(2):617–621. doi: 10.1128/jb.91.2.617-621.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  28. WHITE L. A., KELLOGG D. S., Jr NEISSERIA GONORRHOEAE IDENTIFICATION IN DIRECT SMEARS BY A FLUORESCENT ANTIBODY-COUNTERSTAIN METHOD. Appl Microbiol. 1965 Mar;13:171–174. doi: 10.1128/am.13.2.171-174.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Watson M. E. Compilation of published signal sequences. Nucleic Acids Res. 1984 Jul 11;12(13):5145–5164. doi: 10.1093/nar/12.13.5145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. West S. E., Clark V. L. Genetic loci and linkage associations in Neisseria gonorrhoeae and Neisseria meningitidis. Clin Microbiol Rev. 1989 Apr;2 (Suppl):S92–103. doi: 10.1128/cmr.2.suppl.s92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. West S. E., Iglewski B. H. Codon usage in Pseudomonas aeruginosa. Nucleic Acids Res. 1988 Oct 11;16(19):9323–9335. doi: 10.1093/nar/16.19.9323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Zuerner R. L., Charon N. W. Nucleotide sequence analysis of a gene cloned from Leptospira biflexa serovar patoc which complements an argE defect in Escherichia coli. J Bacteriol. 1988 Oct;170(10):4548–4554. doi: 10.1128/jb.170.10.4548-4554.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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