Abstract
It has been reported by several laboratories that maltose transport in Saccharomyces cerevisiae consists of two components with high- and low-affinity constants for maltose. We have investigated the characteristics of the low-affinity component and have found that it shows an abnormal behavior without similarity to any transport mechanism described in this organism. The results strongly indicate that this apparent transport activity is due not to a genuine transport process but to nonspecific binding of maltose to the cell wall and plasma membrane.
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Selected References
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- Bisson L. F., Fraenkel D. G. Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1730–1734. doi: 10.1073/pnas.80.6.1730. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Busturia A., Lagunas R. Catabolite inactivation of the glucose transport system in Saccharomyces cerevisiae. J Gen Microbiol. 1986 Feb;132(2):379–385. doi: 10.1099/00221287-132-2-379. [DOI] [PubMed] [Google Scholar]
- Cheng Q., Michels C. A. MAL11 and MAL61 encode the inducible high-affinity maltose transporter of Saccharomyces cerevisiae. J Bacteriol. 1991 Mar;173(5):1817–1820. doi: 10.1128/jb.173.5.1817-1820.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cirillo V. P. Relationship between sugar structure and competition for the sugar transport system in Bakers' yeast. J Bacteriol. 1968 Feb;95(2):603–611. doi: 10.1128/jb.95.2.603-611.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE LA FUENTE G., SOLS A. Transport of sugars in yeasts. II. Mechanisms of utilization of disaccharides and related glycosides. Biochim Biophys Acta. 1962 Jan 1;56:49–62. doi: 10.1016/0006-3002(62)90526-7. [DOI] [PubMed] [Google Scholar]
- HOFSTEE B. H. J. On the evaluation of the constants Vm and KM in enzyme reactions. Science. 1952 Sep 26;116(3013):329–331. doi: 10.1126/science.116.3013.329. [DOI] [PubMed] [Google Scholar]
- Heredia C. F., Sols A., DelaFuente G. Specificity of the constitutive hexose transport in yeast. Eur J Biochem. 1968 Aug;5(3):321–329. doi: 10.1111/j.1432-1033.1968.tb00373.x. [DOI] [PubMed] [Google Scholar]
- Khan N. A., Eaton N. R. Purification and characterization of maltase and alpha-methyl glucosidase from yeast. Biochim Biophys Acta. 1967 Sep 12;146(1):173–180. doi: 10.1016/0005-2744(67)90084-8. [DOI] [PubMed] [Google Scholar]
- Loureiro-Dias M. C., Peinado J. M. Transport of maltose in Saccharomyces cerevisiae. Effect of pH and potassium ions. Biochem J. 1984 Sep 1;222(2):293–298. doi: 10.1042/bj2220293. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Novak S., D'Amore T., Stewart G. G. 2-Deoxy-D-glucose resistant yeast with altered sugar transport activity. FEBS Lett. 1990 Aug 20;269(1):202–204. doi: 10.1016/0014-5793(90)81154-g. [DOI] [PubMed] [Google Scholar]
- Ramos J., Szkutnicka K., Cirillo V. P. Characteristics of galactose transport in Saccharomyces cerevisiae cells and reconstituted lipid vesicles. J Bacteriol. 1989 Jun;171(6):3539–3544. doi: 10.1128/jb.171.6.3539-3544.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riezman H. Endocytosis in yeast: several of the yeast secretory mutants are defective in endocytosis. Cell. 1985 Apr;40(4):1001–1009. doi: 10.1016/0092-8674(85)90360-5. [DOI] [PubMed] [Google Scholar]
- Serrano R. Energy requirements for maltose transport in yeast. Eur J Biochem. 1977 Oct 17;80(1):97–102. doi: 10.1111/j.1432-1033.1977.tb11861.x. [DOI] [PubMed] [Google Scholar]
- Waltschewa L., Kotyk A., Venkov P. Increased endocytosis in the Saccharomyces cerevisiae fragile mutant VY1160. Yeast. 1991 Apr;7(3):211–217. doi: 10.1002/yea.320070303. [DOI] [PubMed] [Google Scholar]