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. 1992 Jun;174(12):3850–3854. doi: 10.1128/jb.174.12.3850-3854.1992

Purification and characterization of a novel enoyl coenzyme A reductase from Streptomyces collinus.

K A Reynolds 1, P Wang 1, K M Fox 1, M K Speedie 1, Y Lam 1, H G Floss 1
PMCID: PMC206091  PMID: 1597409

Abstract

A novel NADPH-dependent enoyl reductase, catalyzing the conversion of 1-cyclohexenylcarbonyl coenzyme A (1-cyclohexenylcarbonyl-CoA) to cyclohexylcarbonyl-CoA, was purified to homogeneity from Streptomyces collinus. This enzyme, a dimer with subunits of identical M(r) (36,000), exhibits a Km of 1.5 +/- 0.3 microM for NADPH and 25 +/- 3 microM for 1-cyclohexenylcarbonyl-CoA. It has a pH optimum of 7.5, is most active at 30 degrees C, and is inhibited by both divalent cations and thiol reagents. Two internal peptide sequences were obtained. Ansatrienin A (an antibiotic produced by S. collinus) contains a cyclohexanecarboxylic acid moiety, and it is suggested that the 1-cyclohexenylcarbonyl-CoA reductase described herein catalyzes the final reductive step in the conversion of shikimic acid into this moiety.

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Selected References

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