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. 1992 Jun;174(12):4094–4100. doi: 10.1128/jb.174.12.4094-4100.1992

Escherichia coli K-12 and B contain functional bacteriophage P2 ogr genes.

A Slettan 1, K Gebhardt 1, E Kristiansen 1, N K Birkeland 1, B H Lindqvist 1
PMCID: PMC206121  PMID: 1597424

Abstract

The bacteriophage P2 ogr gene encodes an essential 72-amino-acid protein which acts as a positive regulator of P2 late transcription. A P2 ogr deletion phage, which depends on the supply of Ogr protein in trans for lytic growth on Escherichia coli C, has previously been constructed. E. coli B and K-12 were found to support the growth of the ogr-defective P2 phage because of the presence of functional ogr genes located in cryptic P2-like prophages in these strains. The cryptic ogr genes were cloned and sequenced. Compared with the P2 wild-type ogr gene, the ogr genes in the B and K-12 strains are conserved, containing mostly silent base substitutions. One of the base substitutions in the K-12 ogr gene results in replacement of an alanine with valine at position 57 in the Ogr protein but does not seem to affect the function of Ogr as a transcriptional activator. The cryptic ogr genes are constitutively transcribed, apparently at a higher level than the wild-type ogr gene in a P2 lysogen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ansorge W., Sproat B., Stegemann J., Schwager C., Zenke M. Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis. Nucleic Acids Res. 1987 Jun 11;15(11):4593–4602. doi: 10.1093/nar/15.11.4593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appleyard R K. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. doi: 10.1093/genetics/39.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BERTANI G., WEIGLE J. J. Host controlled variation in bacterial viruses. J Bacteriol. 1953 Feb;65(2):113–121. doi: 10.1128/jb.65.2.113-121.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barreiro V., Haggård-Ljungquist E. Attachment sites for bacteriophage P2 on the Escherichia coli chromosome: DNA sequences, localization on the physical map, and detection of a P2-like remnant in E. coli K-12 derivatives. J Bacteriol. 1992 Jun;174(12):4086–4093. doi: 10.1128/jb.174.12.4086-4093.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Birkeland N. K., Christie G. E., Lindqvist B. H. Directed mutagenesis of the bacteriophage P2 ogr gene defines an essential function. Gene. 1988 Dec 20;73(2):327–335. doi: 10.1016/0378-1119(88)90497-0. [DOI] [PubMed] [Google Scholar]
  6. Birkeland N. K., Lindquist B. H. Coliphage P2 late control gene ogr. DNA sequence and product identification. J Mol Biol. 1986 Apr 5;188(3):487–490. doi: 10.1016/0022-2836(86)90170-1. [DOI] [PubMed] [Google Scholar]
  7. Birkeland N. K., Lindqvist B. H., Christie G. E. Control of bacteriophage P2 gene expression: analysis of transcription of the ogr gene. J Bacteriol. 1991 Nov;173(21):6927–6934. doi: 10.1128/jb.173.21.6927-6934.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. COHEN D. A variant of phage P2 originating in Escherichia coli, strain B. Virology. 1959 Jan;7(1):112–126. doi: 10.1016/0042-6822(59)90180-1. [DOI] [PubMed] [Google Scholar]
  9. Chattoraj D. K., Inman R. B. Electron microscope heteroduplex mapping of P2 Hy dis bacteriophage DNA. Virology. 1973 Sep;55(1):174–182. doi: 10.1016/s0042-6822(73)81019-0. [DOI] [PubMed] [Google Scholar]
  10. Christie G. E., Calendar R. Bacteriophage P2 late promoters. Transcription initiation sites for two late mRNAs. J Mol Biol. 1983 Jul 15;167(4):773–790. doi: 10.1016/s0022-2836(83)80110-7. [DOI] [PubMed] [Google Scholar]
  11. Christie G. E., Calendar R. Interactions between satellite bacteriophage P4 and its helpers. Annu Rev Genet. 1990;24:465–490. doi: 10.1146/annurev.ge.24.120190.002341. [DOI] [PubMed] [Google Scholar]
  12. Christie G. E., Haggård-Ljungquist E., Feiwell R., Calendar R. Regulation of bacteriophage P2 late-gene expression: the ogr gene. Proc Natl Acad Sci U S A. 1986 May;83(10):3238–3242. doi: 10.1073/pnas.83.10.3238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Edlin G., Lin L., Bitner R. Reproductive fitness of P1, P2, and Mu lysogens of Escherichia coli. J Virol. 1977 Feb;21(2):560–564. doi: 10.1128/jvi.21.2.560-564.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Edlin G., Lin L., Kudrna R. Lambda lysogens of E. coli reproduce more rapidly than non-lysogens. Nature. 1975 Jun 26;255(5511):735–737. doi: 10.1038/255735a0. [DOI] [PubMed] [Google Scholar]
  15. Fujiki H., Palm P., Zillig W., Calendar R., Sunshine M. Identification of a mutation within the structural gene for the a subunit of DNA-dependent RNA polymerase of E. coli. Mol Gen Genet. 1976 Apr 23;145(1):19–22. doi: 10.1007/BF00331552. [DOI] [PubMed] [Google Scholar]
  16. Garrett S., Silhavy T. J. Isolation of mutations in the alpha operon of Escherichia coli that suppress the transcriptional defect conferred by a mutation in the porin regulatory gene envZ. J Bacteriol. 1987 Apr;169(4):1379–1385. doi: 10.1128/jb.169.4.1379-1385.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Giffard P. M., Booth I. R. The rpoA341 allele of Escherichia coli specifically impairs the transcription of a group of positively-regulated operons. Mol Gen Genet. 1988 Sep;214(1):148–152. doi: 10.1007/BF00340193. [DOI] [PubMed] [Google Scholar]
  18. Gottesman M. M., Gottesman M. E., Gottesman S., Gellert M. Characterization of bacteriophage lambda reverse as an Escherichia coli phage carrying a unique set of host-derived recombination functions. J Mol Biol. 1974 Sep 15;88(2):471–487. doi: 10.1016/0022-2836(74)90496-3. [DOI] [PubMed] [Google Scholar]
  19. Grambow N. J., Birkeland N. K., Anders D. L., Christie G. E. Deletion analysis of a bacteriophage P2 late promoter. Gene. 1990 Oct 30;95(1):9–15. doi: 10.1016/0378-1119(90)90407-i. [DOI] [PubMed] [Google Scholar]
  20. Grimberg J., Maguire S., Belluscio L. A simple method for the preparation of plasmid and chromosomal E. coli DNA. Nucleic Acids Res. 1989 Nov 11;17(21):8893–8893. doi: 10.1093/nar/17.21.8893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Halling C., Calendar R. Bacteriophage P2 ogr and P4 delta genes act independently and are essential for P4 multiplication. J Bacteriol. 1990 Jul;172(7):3549–3558. doi: 10.1128/jb.172.7.3549-3558.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Henderson D., Weil J. The nature and origin of a class of essential gene substitutions in bacteriophage lambda. Virology. 1975 Sep;67(1):124–135. doi: 10.1016/0042-6822(75)90410-9. [DOI] [PubMed] [Google Scholar]
  23. Higgins C. F., Dorman C. J., Stirling D. A., Waddell L., Booth I. R., May G., Bremer E. A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli. Cell. 1988 Feb 26;52(4):569–584. doi: 10.1016/0092-8674(88)90470-9. [DOI] [PubMed] [Google Scholar]
  24. Igarashi K., Hanamura A., Makino K., Aiba H., Aiba H., Mizuno T., Nakata A., Ishihama A. Functional map of the alpha subunit of Escherichia coli RNA polymerase: two modes of transcription activation by positive factors. Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):8958–8962. doi: 10.1073/pnas.88.20.8958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Igarashi K., Ishihama A. Bipartite functional map of the E. coli RNA polymerase alpha subunit: involvement of the C-terminal region in transcription activation by cAMP-CRP. Cell. 1991 Jun 14;65(6):1015–1022. doi: 10.1016/0092-8674(91)90553-b. [DOI] [PubMed] [Google Scholar]
  26. JACOB F., WOLLMAN E. L. Sur les processus de conjugaison et de recombinaison chez Escherichia coli. I. L'induction par conjugaison ou induction zygotique. Ann Inst Pasteur (Paris) 1956 Oct;91(4):486–510. [PubMed] [Google Scholar]
  27. Kristensen T., Voss H., Schwager C., Stegemann J., Sproat B., Ansorge W. T7 DNA polymerase in automated dideoxy sequencing. Nucleic Acids Res. 1988 Apr 25;16(8):3487–3496. doi: 10.1093/nar/16.8.3487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Kurnit D. M. Escherichia coli recA deletion strains that are highly competent for transformation and for in vivo phage packaging. Gene. 1989 Oct 30;82(2):313–315. doi: 10.1016/0378-1119(89)90056-5. [DOI] [PubMed] [Google Scholar]
  29. Lee T. C., Christie G. E. Purification and properties of the bacteriophage P2 ogr gene product. A prokaryotic zinc-binding transcriptional activator. J Biol Chem. 1990 May 5;265(13):7472–7477. [PubMed] [Google Scholar]
  30. Lichens-Park A., Smith C. L., Syvanen M. Integration of bacteriophage lambda into the cryptic lambdoid prophages of Escherichia coli. J Bacteriol. 1990 May;172(5):2201–2208. doi: 10.1128/jb.172.5.2201-2208.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Lin L., Bitner R., Edlin G. Increased reproductive fitness of Escherichia coli lambda lysogens. J Virol. 1977 Feb;21(2):554–559. doi: 10.1128/jvi.21.2.554-559.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Matsuyama S., Mizushima S. Novel rpoA mutation that interferes with the function of OmpR and EnvZ, positive regulators of the ompF and ompC genes that code for outer-membrane proteins in Escherichia coli K12. J Mol Biol. 1987 Jun 20;195(4):847–853. doi: 10.1016/0022-2836(87)90489-x. [DOI] [PubMed] [Google Scholar]
  33. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Ni Bhriain N., Dorman C. J., Higgins C. F. An overlap between osmotic and anaerobic stress responses: a potential role for DNA supercoiling in the coordinate regulation of gene expression. Mol Microbiol. 1989 Jul;3(7):933–942. doi: 10.1111/j.1365-2958.1989.tb00243.x. [DOI] [PubMed] [Google Scholar]
  35. Rowland G. C., Giffard P. M., Booth I. R. phs Locus of Escherichia coli, a mutation causing pleiotropic lesions in metabolism, is an rpoA allele. J Bacteriol. 1985 Nov;164(2):972–975. doi: 10.1128/jb.164.2.972-975.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  37. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Sasaki I., Bertani G. Growth abnormalities in Hfr derivatives of Escherichia coli strain C. J Gen Microbiol. 1965 Sep;40(3):365–376. doi: 10.1099/00221287-40-3-365. [DOI] [PubMed] [Google Scholar]
  39. Six E. W., Klug C. A. Bacteriophage P4: a satellite virus depending on a helper such as prophage P2. Virology. 1973 Feb;51(2):327–344. doi: 10.1016/0042-6822(73)90432-7. [DOI] [PubMed] [Google Scholar]
  40. Six E. W. The helper dependence of satellite bacteriophage P4: which gene functions of bacteriophage P2 are needed by P4? Virology. 1975 Sep;67(1):249–263. doi: 10.1016/0042-6822(75)90422-5. [DOI] [PubMed] [Google Scholar]
  41. Sun J., Inouye M., Inouye S. Association of a retroelement with a P4-like cryptic prophage (retronphage phi R73) integrated into the selenocystyl tRNA gene of Escherichia coli. J Bacteriol. 1991 Jul;173(13):4171–4181. doi: 10.1128/jb.173.13.4171-4181.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Sunshine M. G., Sauer B. A bacterial mutation blocking P2 phage late gene expression. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2770–2774. doi: 10.1073/pnas.72.7.2770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Vieira J., Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. doi: 10.1016/0076-6879(87)53044-0. [DOI] [PubMed] [Google Scholar]
  44. Westö A., Ljungquist E. A restriction endonuclease cleavage map of bacteriophage P2. Mol Gen Genet. 1979 Mar 9;171(1):91–102. doi: 10.1007/BF00274019. [DOI] [PubMed] [Google Scholar]
  45. Wiman M., Bertani G., Kelly B., Sasaki I. Genetic map of Escherichia coli strain C. Mol Gen Genet. 1970;107(1):1–31. doi: 10.1007/BF00433220. [DOI] [PubMed] [Google Scholar]

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