Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Jun;174(12):4166–4168. doi: 10.1128/jb.174.12.4166-4168.1992

Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.

E M Witkin 1, V Roegner-Maniscalco 1
PMCID: PMC206129  PMID: 1597430

Abstract

A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication and cell viability, as well as the PolI-dependent replication of the plasmid carrying dnaE.

Full text

PDF
4166

Images in this article

4167Image
on p.4167

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Echols H., Goodman M. F. Mutation induced by DNA damage: a many protein affair. Mutat Res. 1990 Sep-Nov;236(2-3):301–311. doi: 10.1016/0921-8777(90)90013-u. [DOI] [PubMed] [Google Scholar]
  2. Fijalkowska I., Jonczyk P., Ciesla Z. Conditional lethality of the recA441 and recA730 mutants of Escherichia coli deficient in DNA polymerase I. Mutat Res. 1989 Mar;217(2):117–122. doi: 10.1016/0921-8777(89)90063-3. [DOI] [PubMed] [Google Scholar]
  3. Gross J. D., Grunstein J., Witkin E. M. Inviability of recA- derivatives of the DNA polymerase mutant of De Lucia and Cairns. J Mol Biol. 1971 Jun 14;58(2):631–634. doi: 10.1016/0022-2836(71)90377-9. [DOI] [PubMed] [Google Scholar]
  4. Joyce C. M., Grindley N. D. Method for determining whether a gene of Escherichia coli is essential: application to the polA gene. J Bacteriol. 1984 May;158(2):636–643. doi: 10.1128/jb.158.2.636-643.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kingsbury D. T., Helinski D. R. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 . J Bacteriol. 1973 Jun;114(3):1116–1124. doi: 10.1128/jb.114.3.1116-1124.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lehman I. R., Uyemura D. G. DNA polymerase I: essential replication enzyme. Science. 1976 Sep 10;193(4257):963–969. doi: 10.1126/science.781842. [DOI] [PubMed] [Google Scholar]
  7. Lieberman H. B., Witkin E. M. Variable expression of the ssb--1 allele in different strains of Escherichia coli K12 and B: differential suppression of its effects on DNA replication, DNA repair and ultraviolet mutagenesis. Mol Gen Genet. 1981;183(2):348–355. doi: 10.1007/BF00270639. [DOI] [PubMed] [Google Scholar]
  8. Maki H., Horiuchi T., Kornberg A. The polymerase subunit of DNA polymerase III of Escherichia coli. I. Amplification of the dnaE gene product and polymerase activity of the alpha subunit. J Biol Chem. 1985 Oct 25;260(24):12982–12986. [PubMed] [Google Scholar]
  9. McCall J. O., Witkin E. M., Kogoma T., Roegner-Maniscalco V. Constitutive expression of the SOS response in recA718 mutants of Escherichia coli requires amplification of RecA718 protein. J Bacteriol. 1987 Feb;169(2):728–734. doi: 10.1128/jb.169.2.728-734.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Monk M., Kinross J. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J Bacteriol. 1972 Mar;109(3):971–978. doi: 10.1128/jb.109.3.971-978.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morimyo M., Shimazu Y. Evidence that the gene uvrB is indispensable for a polymerase I deficient strain of Escherichia coli K-12. Mol Gen Genet. 1976 Sep 23;147(3):243–250. doi: 10.1007/BF00582875. [DOI] [PubMed] [Google Scholar]
  12. Staudenbauer W. L. Structure and replication of the colicin E1 plasmid. Curr Top Microbiol Immunol. 1978;83:93–156. doi: 10.1007/978-3-642-67087-9_3. [DOI] [PubMed] [Google Scholar]
  13. Sweasy J. B., Loeb L. A. Mammalian DNA polymerase beta can substitute for DNA polymerase I during DNA replication in Escherichia coli. J Biol Chem. 1992 Jan 25;267(3):1407–1410. [PubMed] [Google Scholar]
  14. Witkin E. M., McCall J. O., Volkert M. R., Wermundsen I. E. Constitutive expression of SOS functions and modulation of mutagenesis resulting from resolution of genetic instability at or near the recA locus of Escherichia coli. Mol Gen Genet. 1982;185(1):43–50. doi: 10.1007/BF00333788. [DOI] [PubMed] [Google Scholar]
  15. Witkin E. M., Roegner-Maniscalco V., Sweasy J. B., McCall J. O. Recovery from ultraviolet light-induced inhibition of DNA synthesis requires umuDC gene products in recA718 mutant strains but not in recA+ strains of Escherichia coli. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6805–6809. doi: 10.1073/pnas.84.19.6805. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES