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. 1992 Feb;174(3):1072–1075. doi: 10.1128/jb.174.3.1072-1075.1992

Enzymatic basis of thiol-stimulated secretion of porphyrins by Escherichia coli.

G T Javor 1, E F Febre 1
PMCID: PMC206203  PMID: 1732201

Abstract

1-Thioglycerol (TG) stimulates the synthesis of porphyrin in aerobically growing Escherichia coli. Here the levels of delta-aminolevulinate biosynthetic enzymes in untreated and TG-treated E. coli THU and PUC2 (a mutant of THU which overproduces porphyrins in the presence of thiols) cells were determined. TG treatment elevated the activity of glutamyl-tRNA reductase in both strains. The increased activity was not caused by activation of preexisting enzymes by thiols or by oxidizing agents but was dependent on new protein synthesis.

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Selected References

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