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. 1992 Jul;174(13):4500–4503. doi: 10.1128/jb.174.13.4500-4503.1992

Transformation of the gram-positive bacterium Clavibacter xyli subsp. cynodontis by electroporation with plasmids from the IncP incompatibility group.

M C Metzler 1, Y P Zhang 1, T A Chen 1
PMCID: PMC206238  PMID: 1624442

Abstract

We report the transformation of a gram-positive bacterium, Clavibacter xyli subsp. cynodontis, with several plasmids in the IncP incompatibility group from gram-negative bacteria. Our results suggest that IncP plasmids may be transferable to other gram-positive organisms. After optimizing electroporation parameters, we obtained a maximum of 2 x 10(5) transformants per microgram of DNA. The availability of a transformation system for this bacteria will facilitate its use in indirectly expressing beneficial traits in plants.

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Selected References

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