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. 1992 Jul;174(13):4513–4516. doi: 10.1128/jb.174.13.4513-4516.1992

Genetic and morphological characterization of an Escherichia coli chromosome segregation mutant.

P S Stewart 1, R D'Ari 1
PMCID: PMC206241  PMID: 1624443

Abstract

The temperature-sensitive nucleoid segregation mutant of Escherichia coli, PAT32, formerly described as a parA mutant, has been shown to carry a mutation near 66 min on the genetic map. Fine mapping with phages from the collection of Kohara et al. is consistent with its being a parC allele. Observation by fluorescence microscopy revealed the formation, at a nonpermissive temperature, of filaments containing one or two large nucleoids and of normal-size anucleate cells. There was also a significant loss of viability.

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Selected References

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