Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Jul;174(14):4853–4855. doi: 10.1128/jb.174.14.4853-4855.1992

Transfer of conjugal elements in oral black-pigmented Bacteroides (Prevotella) spp. involves DNA rearrangements.

D G Guiney 1, P Hasegawa 1
PMCID: PMC206288  PMID: 1624474

Abstract

Conjugal genetic elements in isolates of oral black-pigmented Bacteroides denticola (Prevotella denticola) and B. intermedius (P. intermedia) transfer tetracycline and penicillin resistance in the absence of plasmids. Transverse alternating-field electrophoresis of restricted chromosomal DNAs from transconjugants revealed arrangements indicating that transfer and insertion can involve more than one 60-kb copy of the elements and occurs at strongly preferred sites in the recipient chromosome.

Full text

PDF
4853

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bedzyk L. A., Shoemaker N. B., Young K. E., Salyers A. A. Insertion and excision of Bacteroides conjugative chromosomal elements. J Bacteriol. 1992 Jan;174(1):166–172. doi: 10.1128/jb.174.1.166-172.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chow A. W., Roser S. M., Brady F. A. Orofacial odontogenic infections. Ann Intern Med. 1978 Mar;88(3):392–402. doi: 10.7326/0003-4819-88-3-392. [DOI] [PubMed] [Google Scholar]
  3. Guiney D. G., Bouic K. Detection of conjugal transfer systems in oral, black-pigmented Bacteroides spp. J Bacteriol. 1990 Jan;172(1):495–497. doi: 10.1128/jb.172.1.495-497.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guiney D. G., Hasegawa P., Bouic K., Matthews B. Genetic transfer systems in Bacteroides: cloning and mapping of the transferable tetracycline-resistance locus. Mol Microbiol. 1989 Nov;3(11):1617–1623. doi: 10.1111/j.1365-2958.1989.tb00147.x. [DOI] [PubMed] [Google Scholar]
  5. Hecht D. W., Malamy M. H. Tn4399, a conjugal mobilizing transposon of Bacteroides fragilis. J Bacteriol. 1989 Jul;171(7):3603–3608. doi: 10.1128/jb.171.7.3603-3608.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hecht D. W., Thompson J. S., Malamy M. H. Characterization of the termini and transposition products of Tn4399, a conjugal mobilizing transposon of Bacteroides fragilis. Proc Natl Acad Sci U S A. 1989 Jul;86(14):5340–5344. doi: 10.1073/pnas.86.14.5340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Matthews B. G., Roudier C., Guiney D. G. A site-specific DNA inversion in Bacteroides plasmid pBF4 is influenced by the presence of the conjugal tetracycline resistance element. J Bacteriol. 1991 Aug;173(16):5239–5243. doi: 10.1128/jb.173.16.5239-5243.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Odelson D. A., Rasmussen J. L., Smith C. J., Macrina F. L. Extrachromosomal systems and gene transmission in anaerobic bacteria. Plasmid. 1987 Mar;17(2):87–109. doi: 10.1016/0147-619x(87)90016-3. [DOI] [PubMed] [Google Scholar]
  9. Salyers A. A., Shoemaker N. B., Guthrie E. P. Recent advances in Bacteroides genetics. Crit Rev Microbiol. 1987;14(1):49–71. doi: 10.3109/10408418709104435. [DOI] [PubMed] [Google Scholar]
  10. Shoemaker N. B., Salyers A. A. A cryptic 65-kilobase-pair transposonlike element isolated from Bacteroides uniformis has homology with Bacteroides conjugal tetracycline resistance elements. J Bacteriol. 1990 Apr;172(4):1694–1702. doi: 10.1128/jb.172.4.1694-1702.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES