Abstract
The denitrifying Pseudomonas strain K172 was grown with a generation time of 6 h to a cell density of 0.4 g (dry weight) per liter with toluene and nitrate as substrates. We found that anaerobic cell suspensions oxidize [14C]toluene first to [14C]benzyl alcohol and subsequently to [14C]benzaldehyde. This proves that the methyl group of toluene is oxidized without molecular oxygen to a hydroxymethyl group.
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Selected References
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- Altenschmidt U., Fuchs G. Anaerobic degradation of toluene in denitrifying Pseudomonas sp.: indication for toluene methylhydroxylation and benzoyl-CoA as central aromatic intermediate. Arch Microbiol. 1991;156(2):152–158. doi: 10.1007/BF00290990. [DOI] [PubMed] [Google Scholar]
- Altenschmidt U., Oswald B., Fuchs G. Purification and characterization of benzoate-coenzyme A ligase and 2-aminobenzoate-coenzyme A ligases from a denitrifying Pseudomonas sp. J Bacteriol. 1991 Sep;173(17):5494–5501. doi: 10.1128/jb.173.17.5494-5501.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dolfing J., Zeyer J., Binder-Eicher P., Schwarzenbach R. P. Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen. Arch Microbiol. 1990;154(4):336–341. doi: 10.1007/BF00276528. [DOI] [PubMed] [Google Scholar]
- Evans P. J., Ling W., Goldschmidt B., Ritter E. R., Young L. Y. Metabolites formed during anaerobic transformation of toluene and o-xylene and their proposed relationship to the initial steps of toluene mineralization. Appl Environ Microbiol. 1992 Feb;58(2):496–501. doi: 10.1128/aem.58.2.496-501.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans P. J., Mang D. T., Kim K. S., Young L. Y. Anaerobic degradation of toluene by a denitrifying bacterium. Appl Environ Microbiol. 1991 Apr;57(4):1139–1145. doi: 10.1128/aem.57.4.1139-1145.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geissler J. F., Harwood C. S., Gibson J. Purification and properties of benzoate-coenzyme A ligase, a Rhodopseudomonas palustris enzyme involved in the anaerobic degradation of benzoate. J Bacteriol. 1988 Apr;170(4):1709–1714. doi: 10.1128/jb.170.4.1709-1714.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gibson K. J., Gibson J. Potential early intermediates in anaerobic benzoate degradation by Rhodopseudomonas palustris. Appl Environ Microbiol. 1992 Feb;58(2):696–698. doi: 10.1128/aem.58.2.696-698.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grbić-Galić D., Vogel T. M. Transformation of toluene and benzene by mixed methanogenic cultures. Appl Environ Microbiol. 1987 Feb;53(2):254–260. doi: 10.1128/aem.53.2.254-260.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hopper D. J. Incorporation of [18O]water in the formation of p-hydroxybenzyl alcohol by the p-cresol methylhydroxylase from Pseudomonas putida. Biochem J. 1978 Oct 1;175(1):345–347. doi: 10.1042/bj1750345. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koch J., Fuchs G. Enzymatic reduction of benzoyl-CoA to alicyclic compounds, a key reaction in anaerobic aromatic metabolism. Eur J Biochem. 1992 Apr 1;205(1):195–202. doi: 10.1111/j.1432-1033.1992.tb16768.x. [DOI] [PubMed] [Google Scholar]
- Lovley D. R., Lonergan D. J. Anaerobic Oxidation of Toluene, Phenol, and p-Cresol by the Dissimilatory Iron-Reducing Organism, GS-15. Appl Environ Microbiol. 1990 Jun;56(6):1858–1864. doi: 10.1128/aem.56.6.1858-1864.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rudolphi A., Tschech A., Fuchs G. Anaerobic degradation of cresols by denitrifying bacteria. Arch Microbiol. 1991;155(3):238–248. doi: 10.1007/BF00252207. [DOI] [PubMed] [Google Scholar]
- Schocher R. J., Seyfried B., Vazquez F., Zeyer J. Anaerobic degradation of toluene by pure cultures of denitrifying bacteria. Arch Microbiol. 1991;157(1):7–12. doi: 10.1007/BF00245327. [DOI] [PubMed] [Google Scholar]
- Stanier R. Y. Simultaneous Adaptation: A New Technique for the Study of Metabolic Pathways. J Bacteriol. 1947 Sep;54(3):339–348. doi: 10.1128/jb.54.3.339-348.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogel T. M., Grbìc-Galìc D. Incorporation of Oxygen from Water into Toluene and Benzene during Anaerobic Fermentative Transformation. Appl Environ Microbiol. 1986 Jul;52(1):200–202. doi: 10.1128/aem.52.1.200-202.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeyer J., Kuhn E. P., Schwarzenbach R. P. Rapid microbial mineralization of toluene and 1,3-dimethylbenzene in the absence of molecular oxygen. Appl Environ Microbiol. 1986 Oct;52(4):944–947. doi: 10.1128/aem.52.4.944-947.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
