Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1998 Jul;78(1):106–110. doi: 10.1038/bjc.1998.450

Serum polysialylated neural cell adhesion molecule in childhood neuroblastoma.

S Glüer 1, C Schelp 1, N Madry 1, D von Schweinitz 1, M Eckhardt 1, R Gerardy-Schahn 1
PMCID: PMC2062928  PMID: 9662259

Abstract

Neuroblastoma cells express the polysialylated form of the neural cell adhesion molecule (NCAM), which normally becomes restricted to a few neural tissues after embryogenesis. In this study, we investigated serum levels of polysialylated NCAM in 14 children with different grades and stages of neuroblastoma using an immunoluminescence assay, and compared the results to 269 healthy control subjects. Simultaneously, the polysialylated NCAM content of the tumours was determined by immunohistochemistry. Serum levels were dramatically elevated (more than sixfold) in children with advanced stages and fatal courses of disease, whereas children with differentiated tumour types and limited disease had low or normal levels. Serum concentrations correlated with the polysialylated NCAM content of the tumours, and they decreased during successful therapy. We therefore suggest polysialylated NCAM to be a useful marker monitoring childhood neuroblastoma.

Full text

PDF
106

Images in this article

107Figure 2
on p.107
107Figure 3
on p.107

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brodeur G. M., Seeger R. C., Schwab M., Varmus H. E., Bishop J. M. Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage. Science. 1984 Jun 8;224(4653):1121–1124. doi: 10.1126/science.6719137. [DOI] [PubMed] [Google Scholar]
  2. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  3. Figarella-Branger D., Dubois C., Chauvin P., De Victor B., Gentet J. C., Rougon G. Correlation between polysialic-neural cell adhesion molecule levels in CSF and medulloblastoma outcomes. J Clin Oncol. 1996 Jul;14(7):2066–2072. doi: 10.1200/JCO.1996.14.7.2066. [DOI] [PubMed] [Google Scholar]
  4. Frosch M., Görgen I., Boulnois G. J., Timmis K. N., Bitter-Suermann D. NZB mouse system for production of monoclonal antibodies to weak bacterial antigens: isolation of an IgG antibody to the polysaccharide capsules of Escherichia coli K1 and group B meningococci. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1194–1198. doi: 10.1073/pnas.82.4.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerardy-Schahn R., Bethe A., Brennecke T., Mühlenhoff M., Eckhardt M., Ziesing S., Lottspeich F., Frosch M. Molecular cloning and functional expression of bacteriophage PK1E-encoded endoneuraminidase Endo NE. Mol Microbiol. 1995 May;16(3):441–450. doi: 10.1111/j.1365-2958.1995.tb02409.x. [DOI] [PubMed] [Google Scholar]
  6. Hughes M., Marsden H. B., Palmer M. K. Histologic patterns of neuroblastoma related to prognosis and clinical staging. Cancer. 1974 Nov;34(5):1706–1711. doi: 10.1002/1097-0142(197411)34:5<1706::aid-cncr2820340519>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  7. Häyrinen J., Bitter-Suermann D., Finne J. Interaction of meningococcal group B monoclonal antibody and its Fab fragment with alpha 2-8-linked sialic acid polymers: requirement of a long oligosaccharide segment for binding. Mol Immunol. 1989 Jun;26(6):523–529. doi: 10.1016/0161-5890(89)90003-5. [DOI] [PubMed] [Google Scholar]
  8. Kaiser U., Jaques G., Havemann K., Auerbach B. Serum NCAM: a potential new prognostic marker for multiple myeloma. Blood. 1994 Feb 1;83(3):871–873. [PubMed] [Google Scholar]
  9. Ledermann J. A., Pasini F., Olabiran Y., Pelosi G. Detection of the neural cell adhesion molecule (NCAM) in serum of patients with small-cell lung cancer (SCLC) with "limited" or "extensive" disease, and bone-marrow infiltration. Int J Cancer Suppl. 1994;8:49–52. doi: 10.1002/ijc.2910570710. [DOI] [PubMed] [Google Scholar]
  10. Moolenaar C. E., Muller E. J., Schol D. J., Figdor C. G., Bock E., Bitter-Suermann D., Michalides R. J. Expression of neural cell adhesion molecule-related sialoglycoprotein in small cell lung cancer and neuroblastoma cell lines H69 and CHP-212. Cancer Res. 1990 Feb 15;50(4):1102–1106. [PubMed] [Google Scholar]
  11. Patel K., Rossell R. J., Bourne S., Moore S. E., Walsh F. S., Kemshead J. T. Monoclonal antibody UJ13A recognizes the neural cell adhesion molecule (NCAM). Int J Cancer. 1989 Dec 15;44(6):1062–1068. doi: 10.1002/ijc.2910440621. [DOI] [PubMed] [Google Scholar]
  12. Rougon G. Structure, metabolism and cell biology of polysialic acids. Eur J Cell Biol. 1993 Aug;61(2):197–207. [PubMed] [Google Scholar]
  13. Smith S. R., Auerbach B., Morgan L. Serum neural cell adhesion molecule in multiple myeloma and other plasma cell disorders. Br J Haematol. 1996 Jan;92(1):67–70. doi: 10.1046/j.1365-2141.1996.00277.x. [DOI] [PubMed] [Google Scholar]
  14. Takamatsu K., Auerbach B., Gerardy-Schahn R., Eckhardt M., Jaques G., Madry N. Characterization of tumor-associated neural cell adhesion molecule in human serum. Cancer Res. 1994 May 15;54(10):2598–2603. [PubMed] [Google Scholar]
  15. Van Camp B., Durie B. G., Spier C., De Waele M., Van Riet I., Vela E., Frutiger Y., Richter L., Grogan T. M. Plasma cells in multiple myeloma express a natural killer cell-associated antigen: CD56 (NKH-1; Leu-19). Blood. 1990 Jul 15;76(2):377–382. [PubMed] [Google Scholar]
  16. Weisgerber C., Husmann M., Frosch M., Rheinheimer C., Peuckert W., Görgen I., Bitter-Suermann D. Embryonic neural cell adhesion molecule in cerebrospinal fluid of younger children: age-dependent decrease during the first year. J Neurochem. 1990 Dec;55(6):2063–2071. doi: 10.1111/j.1471-4159.1990.tb05796.x. [DOI] [PubMed] [Google Scholar]
  17. Yang P., Yin X., Rutishauser U. Intercellular space is affected by the polysialic acid content of NCAM. J Cell Biol. 1992 Mar;116(6):1487–1496. doi: 10.1083/jcb.116.6.1487. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES