Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1992 Aug;174(15):4960–4966. doi: 10.1128/jb.174.15.4960-4966.1992

rec-2-dependent phage recombination in Haemophilus influenzae.

D M Kupfer 1, D McCarthy 1
PMCID: PMC206309  PMID: 1321120

Abstract

The genetic transformation mutant Rd(DB117)rec- has a pleiotropic phenotype that includes reduced levels of phage recombination. Physical mapping experiments showed that this strain has a 78.5-kbp insertion in the rec-2 gene. The rec-2 dependence of phage recombination was reexamined to determine whether the defective phenotype in Rd(DB117)rec- was due to the simple disruption of the rec-2 gene or whether trans-acting factors from the inserted DNA were responsible. Analysis of strains with transposon insertions in the rec-2 gene showed that they were also defective for phage recombination. Therefore, the phage recombination defect was due solely to the disruption of the rec-2 gene. Strain KB6 is proficient for phage recombination but has a defect in genetic transformation resembling that of Rd(DB117)rec-. The transformation defect of KB6 could be complemented by the wild-type rec-2 gene, showing that the rec-2 contributions to genetic transformation and phage recombination were uncoupled in this strain. The rec-2-dependent phenotype of KB6 suggests that the rec-2 gene participates in genetic transformation and phage recombination in different ways.

Full text

PDF
4960

Images in this article

4961Image
on p.4961
4962Image
on p.4962
4963Image
on p.4963
4963Image
on p.4963

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barouki R., Smith H. O. Reexamination of phenotypic defects in rec-1 and rec-2 mutants of Haemophilus influenzae Rd. J Bacteriol. 1985 Aug;163(2):629–634. doi: 10.1128/jb.163.2.629-634.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beattie K. L., Setlow J. K. Transformation-defective strains of Haemophilus influenzae. Nat New Biol. 1971 Jun 9;231(23):177–179. doi: 10.1038/newbio231177a0. [DOI] [PubMed] [Google Scholar]
  3. Boling M. E., Setlow J. K. Dependence of Vegetative Recombination Among Haemophilus influenzae Bacteriophage on the Host Cell. J Virol. 1969 Sep;4(3):240–243. doi: 10.1128/jvi.4.3.240-243.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fitzmaurice W. P., Scocca J. J. Restriction map and location of mutations on the genome of bacteriophage Hp1c1 of Haemophilus influenzae Rd. Gene. 1983 Sep;24(1):29–35. doi: 10.1016/0378-1119(83)90128-2. [DOI] [PubMed] [Google Scholar]
  5. Herriott R. M., Meyer E. M., Vogt M. Defined nongrowth media for stage II development of competence in Haemophilus influenzae. J Bacteriol. 1970 Feb;101(2):517–524. doi: 10.1128/jb.101.2.517-524.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kauc L., Mitchell M., Goodgal S. H. Size and physical map of the chromosome of Haemophilus influenzae. J Bacteriol. 1989 May;171(5):2474–2479. doi: 10.1128/jb.171.5.2474-2479.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lee J. J., Smith H. O., Redfield R. J. Organization of the Haemophilus influenzae Rd genome. J Bacteriol. 1989 Jun;171(6):3016–3024. doi: 10.1128/jb.171.6.3016-3024.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee J. J., Smith H. O. Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis. J Bacteriol. 1988 Sep;170(9):4402–4405. doi: 10.1128/jb.170.9.4402-4405.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCarthy D. Cloning of the rec-2 locus of Haemophilus influenzae. Gene. 1989 Jan 30;75(1):135–143. doi: 10.1016/0378-1119(89)90390-9. [DOI] [PubMed] [Google Scholar]
  10. McCarthy D., Kupfer D. M. Electron microscopy of single-stranded structures in the DNA of competent Haemophilus influenzae cells. J Bacteriol. 1987 Feb;169(2):565–571. doi: 10.1128/jb.169.2.565-571.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McCarthy D. Plasmid recombination in Haemophilus influenzae. J Mol Biol. 1982 Jun 5;157(4):577–596. doi: 10.1016/0022-2836(82)90500-9. [DOI] [PubMed] [Google Scholar]
  12. Notani N. K., Setlow J. K., Joshi V. R., Allison D. P. Molecular basis for the transformation defects in mutants of Haemophilus influenzae. J Bacteriol. 1972 Jun;110(3):1171–1180. doi: 10.1128/jb.110.3.1171-1180.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Notani N. K., Setlow J. K. Mechanism of bacterial transformation and transfection. Prog Nucleic Acid Res Mol Biol. 1974;14(0):39–100. doi: 10.1016/s0079-6603(08)60205-6. [DOI] [PubMed] [Google Scholar]
  14. Setlow J. K., Boling M. E., Beattie K. L., Kimball R. F. A complex of recombination and repair genes in Haemophilus influenzae. J Mol Biol. 1972 Jul 21;68(2):361–378. doi: 10.1016/0022-2836(72)90218-5. [DOI] [PubMed] [Google Scholar]
  15. Setlow J. K., Spikes D., Griffin K. Characterization of the rec-1 gene of Haemophilus influenzae and behavior of the gene in Escherichia coli. J Bacteriol. 1988 Sep;170(9):3876–3881. doi: 10.1128/jb.170.9.3876-3881.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Small G. D. Mechanism of gap-filling during postreplication repair of ultraviolet damage in Haemophilus influenzae. J Bacteriol. 1975 Oct;124(1):176–181. doi: 10.1128/jb.124.1.176-181.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES